##gff-version 3
##sequence-region BBa_M1531 1 9258
BBa_M1531	.	terminator	9130	9258	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1531	.	terminator	9218	9258	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1531	.	polyA_site	9245	9258	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1531	.	engineered_region	9218	9258	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1531	.	stem_loop	9225	9244	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1531	.	stop_codon	9251	9251	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1531	.	terminator	9130	9209	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1531	.	stem_loop	9141	9184	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1531	.	engineered_region	9130	9209	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1531	.	CDS	4785	5504	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1531	.	start_codon	4785	4787	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1531	.	CDS	4785	5504	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1531	.	ribosome_entry_site	423	434	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1531	.	conserved_region	427	430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1531	.	engineered_region	5513	9121	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1531	.	ribosome_entry_site	5513	5526	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1531	.	ribosome_entry_site	7665	7678	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1531	.	CDS	7685	9121	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1531	.	CDS	7685	9118	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1531	.	start_codon	7685	7687	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1531	.	stop_codon	9119	9121	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1531	.	CDS	5533	7656	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1531	.	CDS	5533	7653	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1531	.	start_codon	5533	5535	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1531	.	stop_codon	7654	7656	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1531	.	tag	4593	4778	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1531	.	tag	4593	4778	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1531	.	start_codon	4593	4595	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1531	.	engineered_region	956	4564	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1531	.	ribosome_entry_site	956	969	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1531	.	ribosome_entry_site	3108	3121	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1531	.	CDS	3128	4564	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1531	.	CDS	3128	4561	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1531	.	start_codon	3128	3130	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1531	.	stop_codon	4562	4564	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1531	.	CDS	976	3099	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1531	.	CDS	976	3096	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1531	.	start_codon	976	978	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1531	.	stop_codon	3097	3099	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1531	.	tag	229	414	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1531	.	tag	229	414	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1531	.	start_codon	229	231	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1531	.	CDS	441	947	.	+	0	ID=BBa_M1524;Name=BBa_M1524
BBa_M1531	.	CDS	441	917	.	+	0	ID=annotation2172670;Name=cds;Parent=BBa_M1524
BBa_M1531	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1531	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1531	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1531	.	ribosome_entry_site	4573	4584	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1531	.	conserved_region	4577	4580	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_M1531
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctat
ggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaag
aggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccct
gaccacatcagcataataatactagagaaagaggagaaatactagatggccttcaaaacctgtggtttttcaaaaaaat
ggttagtcatagcagtcattgtcatgtgcctgtgtacggaatgttactgccagtgtacaggtggcgctgattgcacatc
gtgtaccggggcatgtaccgggtgtggcaattgtccgaacgcggttacatgcacaaactcacagcattgcgttaaggca
aacacgtgcacagattctgagaattgcgttaaagctcatacatgcaccggtagtaccgattgtaataccgcgcagacct
gtactaacagcaaggattgcttcgaagcgaatacttgcaccggcagtacgcattgccatcgagctactacgtgtaccaa
ttcgaaagattgctttgaggcaaccacatgcaccggctcctccaattgctataccgcgaccacctgcacagatagcact
aattgttacaaagctacggcgtgcacaaactcttctggctgcccggggcatcatcatcaccatcatcaccatcactagt
actagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctgg
cacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgac
cagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatc
agcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacc
tgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcat
tctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaag
taccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttc
aggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgt
tgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggt
gcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtg
ttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttat
cttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttgg
tccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctgg
tcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagct
ggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaa
accgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctt
tcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtat
ttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaac
ggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtcta
tcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgctt
ttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcag
gtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgt
ccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacac
catcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaac
ccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcaca
agatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgat
ggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtac
cagctgcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttc
ctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacg
aaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcat
tatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactg
accctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaat
ccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctct
gctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaa
ctgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttct
ctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccg
catcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggcc
atcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagc
tggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcct
ggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggcc
agcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcag
aaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcat
caacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaa
aacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatc
tgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgt
gatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggaga
aatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagca
ggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatg
gacggaactcaataaccctgaccacatcagcataataatactagatggctagcaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgct
acatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccga
aggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggt
gatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagt
acaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgcca
caacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttta
ccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttg
agtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagattaaagaggagaatact
agatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaa
cccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtcc
ctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtg
aagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaa
cccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgtt
gcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccc
tggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggt
tcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggt
ctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctgg
cactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaa
ctttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactac
agcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgc
gtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacac
tatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttc
aaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggc
tgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgt
tgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtc
ctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatcc
gttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcat
cgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggtt
ctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacg
tgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgc
gaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggc
ctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgagg
ccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaat
catcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacag
ggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactaga
gattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatgga
gcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatct
ggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgta
atcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaa
tcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgct
gaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacc
cgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccaga
acgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaatacca
aaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtct
cgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaac
aggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcct
gtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggac
tctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctg
gtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccgga
agatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatc
aaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaag
atcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattcc
actgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctg
gaagaaagcgtcaccgaatctctgcacgaacgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata