Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP
This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
HlyA Signal Peptide - Silver Fusion Compatible
Lac Promoter (BBa_R0010) and RBS (B0034)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag
This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).
This is a thermal hysteresis (antifreeze) protein. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1.
The codons were optimized to be used in E. coli, and any restriction enzyme sites were removed.
Composite part for high levels of production of the HlyB and HlyD membrane proteins.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Membrane protein involved in type I secretion.
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
Conversion of the iGEM parts registry to SBOL2.1