LOCUS BBa_M1532 9278 bp DNA linear 23-Dec-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1532 VERSION BBa_M1532.1 FEATURES Location/Qualifiers promoter 1..200 /label=BBa_R0010 RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_K208010 misc_feature 229..414 /label=HlyA misc_feature 229..231 /label=Start misc_feature 229..414 /label=BBa_K208006 misc_feature 427..430 /label=conserved misc_feature 423..434 /label=BBa_B0034 misc_feature 441..443 /label=start CDS 444..920 /label=cds misc_feature 921..944 /label=tag misc_feature 945..947 /label=stop misc_feature 441..947 /label=BBa_M1524 misc_feature 956..969 /label=BBa_B0035 misc_feature 976..978 /label=Start Codon CDS 976..3096 /label=HlyB misc_feature 976..3099 /label=BBa_M1003 misc_feature 3097..3099 /label=Stop Codon misc_feature 3108..3121 /label=BBa_B0035 misc_feature 3128..3130 /label=Start Codon CDS 3128..4561 /label=HlyD misc_feature 3128..4564 /label=BBa_M1004 misc_feature 4562..4564 /label=Stop Codon misc_feature 956..4564 /label=BBa_M1528 misc_feature 4577..4580 /label=conserved misc_feature 4573..4584 /label=BBa_B0034 misc_feature 4593..4778 /label=HlyA misc_feature 4593..4595 /label=Start misc_feature 4593..4778 /label=BBa_K208006 misc_feature 4791..4794 /label=conserved misc_feature 4787..4798 /label=BBa_B0034 misc_feature 4805..4807 /label=Start CDS 4805..5524 /label=GFP Coding Region misc_feature 4805..5524 /label=BBa_K208000 misc_feature 5533..5546 /label=BBa_B0035 misc_feature 5553..5555 /label=Start Codon CDS 5553..7673 /label=HlyB misc_feature 5553..7676 /label=BBa_M1003 misc_feature 7674..7676 /label=Stop Codon misc_feature 7685..7698 /label=BBa_B0035 misc_feature 7705..7707 /label=Start Codon CDS 7705..9138 /label=HlyD misc_feature 7705..9141 /label=BBa_M1004 misc_feature 9139..9141 /label=Stop Codon misc_feature 5533..9141 /label=BBa_M1528 misc_feature 9150..9229 /label=BBa_B0010 stem_loop 9161..9204 /label=stem_loop misc_feature 9238..9278 /label=BBa_B0012 stem_loop 9245..9264 /label=T7 TE polyA_site 9265..9278 /label=polya misc_feature 9271..9271 /label=stop misc_feature 9150..9278 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt agcctatgga 241 agtcagggtg atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt 301 agcttcgatg ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc 361 agtgattttt catatggacg gaactcaata accctgacca catcagcata ataatactag 421 agaaagagga gaaatactag atggccttca aaacctgtgg tttttcaaaa aaatggttag 481 tcatagcagt cattgtcatg tgcctgtgta cggaatgtta ctgccagtgt acaggtggcg 541 ctgattgcac atcgtgtacc ggggcatgta ccgggtgtgg caattgtccg aacgcggtta 601 catgcacaaa ctcacagcat tgcgttaagg caaacacgtg cacagattct gagaattgcg 661 ttaaagctca tacatgcacc ggtagtaccg attgtaatac cgcgcagacc tgtactaaca 721 gcaaggattg cttcgaagcg aatacttgca ccggcagtac gcattgccat cgagctacta 781 cgtgtaccaa ttcgaaagat tgctttgagg caaccacatg caccggctcc tccaattgct 841 ataccgcgac cacctgcaca gatagcacta attgttacaa agctacggcg tgcacaaact 901 cttctggctg cccggggcat catcatcacc atcatcacca tcaccagtac tagagattaa 961 agaggagaat actagatgga ttcctgtcac aagattgatt acggcctgta tgcactggaa 1021 atcctggcac aatatcataa cgtctccgtc aacccggagg aaattaaaca tcgcttcgat 1081 actgacggca ctggcctggg tctgaccagc tggctgctgg cagcgaagtc cctggagctg 1141 aaggtaaagc aagtgaaaaa gacgattgat cgtctgaact tcatcagcct gccggcactg 1201 gtttggcgtg aagatggccg tcacttcatc ctgacgaaag tgtccaaaga agcgaaccgc 1261 tacctgatct ttgatctgga gcagcgcaac ccgcgcgtac tggaacagag cgagttcgag 1321 gctctgtatc agggccacat cattctgatt gcatctcgtt cttctgttgc aggtaaactg 1381 gcgaaattcg acttcacctg gttcattccg gctattatca agtaccgtcg tattttcatc 1441 gaaaccctgg ttgtatctgt tttcctgcaa ctgttcgctc tgattactcc gctgttcttt 1501 caggttgtca tggacaaagt tctggttcac cgtggctttt ccaccctgaa cgtcattact 1561 gttgcgctga gcgttgttgt tgtgttcgaa atcattctgt ccggtctgcg tacctacatc 1621 ttcgcgcact ctacctcccg catcgacgtt gaactgggtg cgaagctgtt ccgtcacctg 1681 ctggcactgc ctatcagcta cttcgaatct cgtcgtgtgg gcgatactgt ggcccgtgtt 1741 cgtgaactgg atcagatccg caactttctg accggccagg ccctgaccag cgttctggat 1801 ctgctgttca gctttatctt cttcgcagta atgtggtact acagcccgaa gctgactctg 1861 gtgattctgt tctctctgcc gtgttacgca gcttggtccg tcttcatctc tccaatcctg 1921 cgtcgccgtc tggacgacaa atttagccgt aatgcggata atcagtcctt cctggtcgaa 1981 tctgtgactg ctatcaacac tatcaaagcc atggcggttt ctccacagat gactaacatc 2041 tgggataagc agctggcagg ttacgttgct gcgggcttca aagtgaccgt tctggcaacc 2101 attggtcaac agggcattca gctgattcag aaaaccgtga tgatcattaa cctgtggctg 2161 ggtgcgcacc tggtcatcag cggtgacctg tccattggcc agctgatcgc tttcaacatg 2221 ctggcgggtc aaattgttgc cccagtaatc cgtctggccc agatctggca ggacttccag 2281 caggtcggta tttctgtgac ccgcctgggt gacgtcctga actctccgac cgaaagctac 2341 cacggtaaac tggctctgcc ggagatcaac ggcgatatta ctttccgtaa catccgtttt 2401 cgttacaaac cggattcccc ggtaatcctg gataacatca atctgtctat caaacagggc 2461 gaagtcatcg gcatcgtggg ccgtagcggc agcggcaagt ccactctgac taaactgatt 2521 cagcgctttt acattccaga aaacggtcag gttctgattg acggtcacga cctggctctg 2581 gcggacccga actggctgcg tcgtcaggtg ggcgtagtgc tgcaagataa cgtgctgctg 2641 aaccgtagca tcatcgataa catcagcctg gctaacccgg gcatgtccgt ggaaaaagtt 2701 atctatgctg cgaaactggc aggcgcgcac gatttcatct ctgaactgcg tgaaggctac 2761 aacaccatcg taggcgaaca gggcgctggc ctgtccggcg gtcaacgtca gcgtatcgct 2821 attgcacgtg cactggtgaa caacccgaaa atcctgatct tcgacgaggc cacttctgct 2881 ctggattacg aatctgaaca tattatcatg cgtaatatgc acaagatctg caaaggtcgc 2941 accgtaatca tcatcgccca ccgtctgagc accgtaaaga acgccgaccg catcatcgtg 3001 atggaaaaag gtaagatcgt tgaacagggt aaacataaag agctgctgtc cgaaccggaa 3061 tctctgtatt cttatctgta ccagctgcaa tctgattaat actagagatt aaagaggaga 3121 atactagatg aaaacttggc tgatgggctt ctctgagttc ctgctgcgtt ataagctggt 3181 atggagcgaa acttggaaaa tccgtaagca actggatacc ccggtacgtg aaaaagacga 3241 aaacgaattt ctgccagcac atctggaact gattgagacg ccggtttctc gccgtccacg 3301 tctggttgct tacttcatta tgggcttcct ggttattgct gtaatcctgt ctgttctggg 3361 tcaggtggaa atcgttgcga ccgcgaatgg taaactgacc ctgtccggtc gttctaaaga 3421 aatcaaaccg atcgaaaact ccatcgttaa agagattatc gttaaagaag gtgaatccgt 3481 acgcaaaggc gatgttctgc tgaaactgac cgcactgggc gcagaagcgg acactctgaa 3541 aactcagtcc tctctgctgc aaacccgtct ggaacagacc cgttaccaaa tcctgagccg 3601 ttctattgaa ctgaacaaac tgccggaact gaaactgccg gatgaaccat acttccagaa 3661 cgtaagcgag gaggaagttc tgcgcctgac ttccctgatc aaagagcagt tctctacttg 3721 gcagaaccag aaataccaaa aggaactgaa cctggacaaa aagcgtgcgg agcgcctgac 3781 tatcctggcc cgcatcaacc gttacgagaa cctgtctcgt gttgaaaaat ctcgcctgga 3841 cgacttccgt agcctgctgc acaaacaggc catcgcaaaa cacgctgtgc tggaacagga 3901 aaataaatac gtggaagctg ccaacgaact gcgtgtatat aaaagccagc tggagcagat 3961 cgagagcgaa atcctgtctg cgaaggagga gtaccagctg gtgacgcagc tgttcaaaaa 4021 tgaaatcctg gacaaactgc gtcaaaccac ggactctatc gaactgctga ccctggaact 4081 ggagaaaaac gaggagcgcc agcaggccag cgtcatccgc gctccggtat ctggtaaagt 4141 tcagcaactg aaagttcaca ccgaaggtgg cgttgttacc accgcagaaa ccctgatggt 4201 tattgtgccg gaagatgaca ccctggaagt taccgctctg gtgcagaaca aagatatcgg 4261 tttcatcaac gtcggtcaga acgcgatcat caaagtagag gctttcccgt acactcgtta 4321 cggctacctg gtgggcaagg tcaaaaacat caacctggac gctatcgaag atcagaaact 4381 gggcctggtg ttcaacgtga tcgttagcgt agaagagaac gatctgtcta ccggtaacaa 4441 acacattcca ctgtcctccg gcatggcggt tactgctgaa atcaaaaccg gtatgcgtag 4501 cgtgatctcc tatctgctgt ctccgctgga agaaagcgtc accgaatctc tgcacgaacg 4561 ctaatactag agaaagagga gaaatactag agttagccta tggaagtcag ggtgatctta 4621 atccattaat taatgaaatc agcaaaatca tttcagcagc aggtagcttc gatgttaaag 4681 aggaaagaac cgcagcttct ttattgcagt tgtccggtaa tgccagtgat ttttcatatg 4741 gacggaactc aataaccctg accacatcag cataataata ctagagaaag aggagaaata 4801 ctagatggct agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 4861 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac 4921 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 4981 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat 5041 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat 5101 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac 5161 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg 5221 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa 5281 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact 5341 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 5401 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat 5461 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa 5521 ataatactag agattaaaga ggagaatact agatggattc ctgtcacaag attgattacg 5581 gcctgtatgc actggaaatc ctggcacaat atcataacgt ctccgtcaac ccggaggaaa 5641 ttaaacatcg cttcgatact gacggcactg gcctgggtct gaccagctgg ctgctggcag 5701 cgaagtccct ggagctgaag gtaaagcaag tgaaaaagac gattgatcgt ctgaacttca 5761 tcagcctgcc ggcactggtt tggcgtgaag atggccgtca cttcatcctg acgaaagtgt 5821 ccaaagaagc gaaccgctac ctgatctttg atctggagca gcgcaacccg cgcgtactgg 5881 aacagagcga gttcgaggct ctgtatcagg gccacatcat tctgattgca tctcgttctt 5941 ctgttgcagg taaactggcg aaattcgact tcacctggtt cattccggct attatcaagt 6001 accgtcgtat tttcatcgaa accctggttg tatctgtttt cctgcaactg ttcgctctga 6061 ttactccgct gttctttcag gttgtcatgg acaaagttct ggttcaccgt ggcttttcca 6121 ccctgaacgt cattactgtt gcgctgagcg ttgttgttgt gttcgaaatc attctgtccg 6181 gtctgcgtac ctacatcttc gcgcactcta cctcccgcat cgacgttgaa ctgggtgcga 6241 agctgttccg tcacctgctg gcactgccta tcagctactt cgaatctcgt cgtgtgggcg 6301 atactgtggc ccgtgttcgt gaactggatc agatccgcaa ctttctgacc ggccaggccc 6361 tgaccagcgt tctggatctg ctgttcagct ttatcttctt cgcagtaatg tggtactaca 6421 gcccgaagct gactctggtg attctgttct ctctgccgtg ttacgcagct tggtccgtct 6481 tcatctctcc aatcctgcgt cgccgtctgg acgacaaatt tagccgtaat gcggataatc 6541 agtccttcct ggtcgaatct gtgactgcta tcaacactat caaagccatg gcggtttctc 6601 cacagatgac taacatctgg gataagcagc tggcaggtta cgttgctgcg ggcttcaaag 6661 tgaccgttct ggcaaccatt ggtcaacagg gcattcagct gattcagaaa accgtgatga 6721 tcattaacct gtggctgggt gcgcacctgg tcatcagcgg tgacctgtcc attggccagc 6781 tgatcgcttt caacatgctg gcgggtcaaa ttgttgcccc agtaatccgt ctggcccaga 6841 tctggcagga cttccagcag gtcggtattt ctgtgacccg cctgggtgac gtcctgaact 6901 ctccgaccga aagctaccac ggtaaactgg ctctgccgga gatcaacggc gatattactt 6961 tccgtaacat ccgttttcgt tacaaaccgg attccccggt aatcctggat aacatcaatc 7021 tgtctatcaa acagggcgaa gtcatcggca tcgtgggccg tagcggcagc ggcaagtcca 7081 ctctgactaa actgattcag cgcttttaca ttccagaaaa cggtcaggtt ctgattgacg 7141 gtcacgacct ggctctggcg gacccgaact ggctgcgtcg tcaggtgggc gtagtgctgc 7201 aagataacgt gctgctgaac cgtagcatca tcgataacat cagcctggct aacccgggca 7261 tgtccgtgga aaaagttatc tatgctgcga aactggcagg cgcgcacgat ttcatctctg 7321 aactgcgtga aggctacaac accatcgtag gcgaacaggg cgctggcctg tccggcggtc 7381 aacgtcagcg tatcgctatt gcacgtgcac tggtgaacaa cccgaaaatc ctgatcttcg 7441 acgaggccac ttctgctctg gattacgaat ctgaacatat tatcatgcgt aatatgcaca 7501 agatctgcaa aggtcgcacc gtaatcatca tcgcccaccg tctgagcacc gtaaagaacg 7561 ccgaccgcat catcgtgatg gaaaaaggta agatcgttga acagggtaaa cataaagagc 7621 tgctgtccga accggaatct ctgtattctt atctgtacca gctgcaatct gattaatact 7681 agagattaaa gaggagaata ctagatgaaa acttggctga tgggcttctc tgagttcctg 7741 ctgcgttata agctggtatg gagcgaaact tggaaaatcc gtaagcaact ggataccccg 7801 gtacgtgaaa aagacgaaaa cgaatttctg ccagcacatc tggaactgat tgagacgccg 7861 gtttctcgcc gtccacgtct ggttgcttac ttcattatgg gcttcctggt tattgctgta 7921 atcctgtctg ttctgggtca ggtggaaatc gttgcgaccg cgaatggtaa actgaccctg 7981 tccggtcgtt ctaaagaaat caaaccgatc gaaaactcca tcgttaaaga gattatcgtt 8041 aaagaaggtg aatccgtacg caaaggcgat gttctgctga aactgaccgc actgggcgca 8101 gaagcggaca ctctgaaaac tcagtcctct ctgctgcaaa cccgtctgga acagacccgt 8161 taccaaatcc tgagccgttc tattgaactg aacaaactgc cggaactgaa actgccggat 8221 gaaccatact tccagaacgt aagcgaggag gaagttctgc gcctgacttc cctgatcaaa 8281 gagcagttct ctacttggca gaaccagaaa taccaaaagg aactgaacct ggacaaaaag 8341 cgtgcggagc gcctgactat cctggcccgc atcaaccgtt acgagaacct gtctcgtgtt 8401 gaaaaatctc gcctggacga cttccgtagc ctgctgcaca aacaggccat cgcaaaacac 8461 gctgtgctgg aacaggaaaa taaatacgtg gaagctgcca acgaactgcg tgtatataaa 8521 agccagctgg agcagatcga gagcgaaatc ctgtctgcga aggaggagta ccagctggtg 8581 acgcagctgt tcaaaaatga aatcctggac aaactgcgtc aaaccacgga ctctatcgaa 8641 ctgctgaccc tggaactgga gaaaaacgag gagcgccagc aggccagcgt catccgcgct 8701 ccggtatctg gtaaagttca gcaactgaaa gttcacaccg aaggtggcgt tgttaccacc 8761 gcagaaaccc tgatggttat tgtgccggaa gatgacaccc tggaagttac cgctctggtg 8821 cagaacaaag atatcggttt catcaacgtc ggtcagaacg cgatcatcaa agtagaggct 8881 ttcccgtaca ctcgttacgg ctacctggtg ggcaaggtca aaaacatcaa cctggacgct 8941 atcgaagatc agaaactggg cctggtgttc aacgtgatcg ttagcgtaga agagaacgat 9001 ctgtctaccg gtaacaaaca cattccactg tcctccggca tggcggttac tgctgaaatc 9061 aaaaccggta tgcgtagcgt gatctcctat ctgctgtctc cgctggaaga aagcgtcacc 9121 gaatctctgc acgaacgcta atactagagc caggcatcaa ataaaacgaa aggctcagtc 9181 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 9241 cactggctca ccttcgggtg ggcctttctg cgtttata //