BBa_M1532
type: ComponentDefinition
persistent identity: BBa M1532
display id: BBa_M1532
version: 1
was generated by: iGEM to SBOL conversion
description: Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP
created: 2012-04-17T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12290
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: See subparts
star: false
creator: Sarah Allred
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_K208010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K208006
sequence annotation: Start
sequence annotation: HlyA
sequence annotation: conserved
sequence annotation: start
sequence annotation: BBa_M1524
sequence annotation: cds
sequence annotation: tag
sequence annotation: stop
sequence annotation: BBa_B0035
sequence annotation: BBa_M1528
sequence annotation: Start Codon
sequence annotation: BBa_M1003
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence annotation: BBa_M1004
sequence annotation: HlyD
sequence annotation: BBa_K208000
sequence annotation: GFP Coding Region
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2172447
display id: annotation2172447
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172447/range2172447/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172447
display id: range2172447
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2172444
display id: annotation2172444
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172444/range2172444/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1532/annotation2172444/range2172444/1
type: Range
persistent identity: range2172444
display id: range2172444
version: 1
top level: BBa_M1532
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172446
display id: annotation2172446
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172446/range2172446/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2172446
display id: range2172446
version: 1
top level: BBa_M1532
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172450
display id: annotation2172450
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172450/range2172450/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172450
display id: range2172450
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 229
end: 414
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172449
display id: annotation2172449
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172449/range2172449/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172449
display id: range2172449
version: 1
top level: BBa_M1532
direction: direction/forward
start: 229
end: 231
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2172448
display id: annotation2172448
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172448/range2172448/1
role: SO:0000324
role: feature/tag

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type: Range
persistent identity: range2172448
display id: range2172448
version: 1
top level: BBa_M1532
direction: direction/forward
start: 229
end: 414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172452
display id: annotation2172452
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172452/range2172452/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172452
display id: range2172452
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 423
end: 434
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172451
display id: annotation2172451
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172451/range2172451/1
role: feature/conserved
role: SO:0000330

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type: Range
persistent identity: range2172451
display id: range2172451
version: 1
top level: BBa_M1532
direction: direction/forward
start: 427
end: 430
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2172453
display id: annotation2172453
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172453/range2172453/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172453
display id: range2172453
version: 1
top level: BBa_M1532
direction: direction/forward
start: 441
end: 443
orientation: inline

BBa_M1524
type: SequenceAnnotation
persistent identity: annotation2172457
display id: annotation2172457
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172457/range2172457/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172457
display id: range2172457
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 441
end: 947
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2172454
display id: annotation2172454
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172454/range2172454/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2172454
display id: range2172454
version: 1
top level: BBa_M1532
direction: direction/forward
start: 444
end: 920
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2172455
display id: annotation2172455
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172455/range2172455/1
role: SO:0000324
role: feature/tag

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type: Range
persistent identity: range2172455
display id: range2172455
version: 1
top level: BBa_M1532
direction: direction/forward
start: 921
end: 944
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2172456
display id: annotation2172456
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172456/range2172456/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2172456
display id: range2172456
version: 1
top level: BBa_M1532
direction: direction/forward
start: 945
end: 947
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172458
display id: annotation2172458
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172458/range2172458/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172458
display id: range2172458
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 956
end: 969
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172468
display id: annotation2172468
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172468/range2172468/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172468
display id: range2172468
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 956
end: 4564
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172459
display id: annotation2172459
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172459/range2172459/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172459
display id: range2172459
version: 1
top level: BBa_M1532
direction: direction/forward
start: 976
end: 978
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172461
display id: annotation2172461
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172461/range2172461/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172461
display id: range2172461
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 976
end: 3099
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2172460
display id: annotation2172460
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172460/range2172460/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2172460
display id: range2172460
version: 1
top level: BBa_M1532
direction: direction/forward
start: 976
end: 3096
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172462
display id: annotation2172462
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172462/range2172462/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1532/annotation2172462/range2172462/1
type: Range
persistent identity: range2172462
display id: range2172462
version: 1
top level: BBa_M1532
direction: direction/forward
start: 3097
end: 3099
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172463
display id: annotation2172463
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172463/range2172463/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172463
display id: range2172463
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 3108
end: 3121
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172466
display id: annotation2172466
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172466/range2172466/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1532/annotation2172466/range2172466/1
type: Range
persistent identity: range2172466
display id: range2172466
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 3128
end: 4564
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2172465
display id: annotation2172465
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172465/range2172465/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2172465
display id: range2172465
version: 1
top level: BBa_M1532
direction: direction/forward
start: 3128
end: 4561
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172464
display id: annotation2172464
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172464/range2172464/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2172464
display id: range2172464
version: 1
top level: BBa_M1532
direction: direction/forward
start: 3128
end: 3130
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172467
display id: annotation2172467
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172467/range2172467/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2172467
display id: range2172467
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4562
end: 4564
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172470
display id: annotation2172470
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172470/range2172470/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172470
display id: range2172470
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 4573
end: 4584
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172469
display id: annotation2172469
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172469/range2172469/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range2172469
display id: range2172469
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4577
end: 4580
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172473
display id: annotation2172473
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172473/range2172473/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172473
display id: range2172473
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 4593
end: 4778
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172472
display id: annotation2172472
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172472/range2172472/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172472
display id: range2172472
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4593
end: 4595
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2172471
display id: annotation2172471
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172471/range2172471/1
role: SO:0000324
role: feature/tag

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type: Range
persistent identity: range2172471
display id: range2172471
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4593
end: 4778
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172475
display id: annotation2172475
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172475/range2172475/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172475
display id: range2172475
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 4787
end: 4798
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172474
display id: annotation2172474
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172474/range2172474/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range2172474
display id: range2172474
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4791
end: 4794
orientation: inline

BBa_K208000
type: SequenceAnnotation
persistent identity: annotation2172478
display id: annotation2172478
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172478/range2172478/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172478
display id: range2172478
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 4805
end: 5524
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2172477
display id: annotation2172477
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172477/range2172477/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range2172477
display id: range2172477
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4805
end: 5524
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172476
display id: annotation2172476
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172476/range2172476/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172476
display id: range2172476
version: 1
top level: BBa_M1532
direction: direction/forward
start: 4805
end: 4807
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172479
display id: annotation2172479
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172479/range2172479/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172479
display id: range2172479
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 5533
end: 5546
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172489
display id: annotation2172489
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172489/range2172489/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172489
display id: range2172489
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 5533
end: 9141
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172482
display id: annotation2172482
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172482/range2172482/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172482
display id: range2172482
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 5553
end: 7676
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2172481
display id: annotation2172481
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172481/range2172481/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2172481
display id: range2172481
version: 1
top level: BBa_M1532
direction: direction/forward
start: 5553
end: 7673
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172480
display id: annotation2172480
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172480/range2172480/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2172480
display id: range2172480
version: 1
top level: BBa_M1532
direction: direction/forward
start: 5553
end: 5555
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172483
display id: annotation2172483
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172483/range2172483/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1532/annotation2172483/range2172483/1
type: Range
persistent identity: range2172483
display id: range2172483
version: 1
top level: BBa_M1532
direction: direction/forward
start: 7674
end: 7676
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172484
display id: annotation2172484
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172484/range2172484/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1532/annotation2172484/range2172484/1
type: Range
persistent identity: range2172484
display id: range2172484
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 7685
end: 7698
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172487
display id: annotation2172487
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172487/range2172487/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1532/annotation2172487/range2172487/1
type: Range
persistent identity: range2172487
display id: range2172487
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 7705
end: 9141
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2172486
display id: annotation2172486
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172486/range2172486/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1532/annotation2172486/range2172486/1
type: Range
persistent identity: range2172486
display id: range2172486
version: 1
top level: BBa_M1532
direction: direction/forward
start: 7705
end: 9138
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172485
display id: annotation2172485
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172485/range2172485/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1532/annotation2172485/range2172485/1
type: Range
persistent identity: range2172485
display id: range2172485
version: 1
top level: BBa_M1532
direction: direction/forward
start: 7705
end: 7707
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172488
display id: annotation2172488
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172488/range2172488/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1532/annotation2172488/range2172488/1
type: Range
persistent identity: range2172488
display id: range2172488
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9139
end: 9141
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2172496
display id: annotation2172496
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172496/range2172496/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1532/annotation2172496/range2172496/1
type: Range
persistent identity: range2172496
display id: range2172496
version: 1
top level: BBa_M1532
direction: direction/reverse
start: 9150
end: 9278
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2172490
display id: annotation2172490
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172490/range2172490/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1532/annotation2172490/range2172490/1
type: Range
persistent identity: range2172490
display id: range2172490
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9150
end: 9229
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2172491
display id: annotation2172491
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172491/range2172491/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_M1532/annotation2172491/range2172491/1
type: Range
persistent identity: range2172491
display id: range2172491
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9161
end: 9204
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2172492
display id: annotation2172492
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172492/range2172492/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1532/annotation2172492/range2172492/1
type: Range
persistent identity: range2172492
display id: range2172492
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9238
end: 9278
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2172493
display id: annotation2172493
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172493/range2172493/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_M1532/annotation2172493/range2172493/1
type: Range
persistent identity: range2172493
display id: range2172493
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9245
end: 9264
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2172494
display id: annotation2172494
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172494/range2172494/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_M1532/annotation2172494/range2172494/1
type: Range
persistent identity: range2172494
display id: range2172494
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9265
end: 9278
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2172495
display id: annotation2172495
version: 1
top level: BBa_M1532
location: https://synbiohub.org/public/igem/BBa_M1532/annotation2172495/range2172495/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1532/annotation2172495/range2172495/1
type: Range
persistent identity: range2172495
display id: range2172495
version: 1
top level: BBa_M1532
direction: direction/forward
start: 9271
end: 9271
orientation: inline

https://synbiohub.org/public/igem/BBa_M1532_sequence/1
type: Sequence
persistent identity: BBa M1532 sequence
display id: BBa_M1532_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatggccttcaaaacctgtggtttttcaaaaaaatggttagtcatagcagtcattgtcatgtgcctgtgtacggaatgttactgccagtgtacaggtggcgctgattgcacatcgtgtaccggggcatgtaccgggtgtggcaattgtccgaacgcggttacatgcacaaactcacagcattgcgttaaggcaaacacgtgcacagattctgagaattgcgttaaagctcatacatgcaccggtagtaccgattgtaataccgcgcagacctgtactaacagcaaggattgcttcgaagcgaatacttgcaccggcagtacgcattgccatcgagctactacgtgtaccaattcgaaagattgctttgaggcaaccacatgcaccggctcctccaattgctataccgcgaccacctgcacagatagcactaattgttacaaagctacggcgtgcacaaactcttctggctgcccggggcatcatcatcaccatcatcaccatcaccagtactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacac…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z