BBa_M1533 1 BBa_M1533 Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP 2012-04-17T11:00:00Z 2015-05-08T01:13:56Z See subparts This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter. true false _768_ 0 12290 9 Discontinued false See subparts false Sarah Allred annotation2172513 1 HlyB range2172513 1 976 3096 annotation2172536 1 Stop Codon range2172536 1 7674 7676 annotation2172525 1 Start range2172525 1 4593 4595 annotation2172540 1 BBa_M1004 range2172540 1 7705 9141 annotation2172515 1 Stop Codon range2172515 1 3097 3099 annotation2172531 1 BBa_K208000 range2172531 1 4805 5524 annotation2172546 1 T7 TE range2172546 1 9245 9264 annotation2172523 1 BBa_B0034 range2172523 1 4573 4584 annotation2172500 1 BBa_K208010 range2172500 1 1 220 annotation2172507 1 cds range2172507 1 444 920 annotation2172537 1 BBa_B0035 range2172537 1 7685 7698 annotation2172548 1 stop range2172548 1 9271 9271 annotation2172502 1 Start range2172502 1 229 231 annotation2172541 1 Stop Codon range2172541 1 9139 9141 annotation2172529 1 Start range2172529 1 4805 4807 annotation2172505 1 BBa_B0034 range2172505 1 423 434 annotation2172538 1 Start Codon range2172538 1 7705 7707 annotation2172528 1 BBa_B0034 range2172528 1 4787 4798 annotation2172509 1 stop range2172509 1 945 947 annotation2172526 1 BBa_K208006 range2172526 1 4593 4778 annotation2172520 1 Stop Codon range2172520 1 4562 4564 annotation2172542 1 BBa_M1528 range2172542 1 5533 9141 annotation2172501 1 HlyA range2172501 1 229 414 annotation2172533 1 Start Codon range2172533 1 5553 5555 annotation2172521 1 BBa_M1528 range2172521 1 956 4564 annotation2172547 1 polya range2172547 1 9265 9278 annotation2172527 1 conserved range2172527 1 4791 4794 annotation2172530 1 GFP Coding Region range2172530 1 4805 5524 annotation2172503 1 BBa_K208006 range2172503 1 229 414 annotation2172504 1 conserved range2172504 1 427 430 annotation2172544 1 stem_loop range2172544 1 9161 9204 annotation2172512 1 Start Codon range2172512 1 976 978 annotation2172543 1 BBa_B0010 range2172543 1 9150 9229 annotation2172506 1 start range2172506 1 441 443 annotation2172517 1 Start Codon range2172517 1 3128 3130 annotation2172499 1 BBa_B0034 range2172499 1 209 220 annotation2172524 1 HlyA range2172524 1 4593 4778 annotation2172518 1 HlyD range2172518 1 3128 4561 annotation2172539 1 HlyD range2172539 1 7705 9138 annotation2172522 1 conserved range2172522 1 4577 4580 annotation2172549 1 BBa_B0015 range2172549 1 9150 9278 annotation2172510 1 BBa_M1525 range2172510 1 441 947 annotation2172508 1 tag range2172508 1 921 944 annotation2172534 1 HlyB range2172534 1 5553 7673 annotation2172497 1 BBa_R0010 range2172497 1 1 200 annotation2172511 1 BBa_B0035 range2172511 1 956 969 annotation2172514 1 BBa_M1003 range2172514 1 976 3099 annotation2172516 1 BBa_B0035 range2172516 1 3108 3121 annotation2172535 1 BBa_M1003 range2172535 1 5553 7676 annotation2172545 1 BBa_B0012 range2172545 1 9238 9278 annotation2172532 1 BBa_B0035 range2172532 1 5533 5546 annotation2172519 1 BBa_M1004 range2172519 1 3128 4564 BBa_M1533_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatgtcttttaaaattagcacctttaccaaaatctggctcattatcgcggtcatcgtcatgtgtctgtgcaatgaatacaactgtcagtgtacgggggccgcagattgcacatcgtgcacggcagcatgcactggttgtggcaattgtccgaacgcgattacctgtaccggaagcaagaattgcgtgcgcgcaacaacttgtactgatagcgaaaactgcgttaaagcacatacttgtacaggatcgacaaattgcaacagagcgaccacctgtactaacagcaaggggtgcttggaggccacgacgtgcactggttctactcattgtcatcgcgcaactacgtgcacaaatagcaaagattgttttgaagctaccacatgtactgggtcatcaaactgctataccgctaccacctgcactaatagcacgaactgctataaagctactgcatgtacaaatagtacggggtgcccagggcatcatcatcaccatcatcaccatcaccagtactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggagaaatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttct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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z