##gff-version 3
##sequence-region BBa_M1533 1 9278
BBa_M1533	.	engineered_region	5553	7676	.	+	0	ID=annotation2172535;Name=BBa_M1003
BBa_M1533	.	polyA_site	9265	9278	.	+	0	ID=annotation2172547;Name=polya
BBa_M1533	.	CDS	976	3096	.	+	0	ID=annotation2172513;Name=HlyB
BBa_M1533	.	engineered_region	3128	4564	.	+	0	ID=annotation2172519;Name=BBa_M1004
BBa_M1533	.	stop_codon	7674	7676	.	+	0	ID=annotation2172536;Name=Stop Codon
BBa_M1533	.	CDS	444	920	.	+	0	ID=annotation2172507;Name=cds
BBa_M1533	.	stop_codon	9139	9141	.	+	0	ID=annotation2172541;Name=Stop Codon
BBa_M1533	.	engineered_region	5533	5546	.	+	0	ID=annotation2172532;Name=BBa_B0035
BBa_M1533	.	engineered_region	5533	9141	.	+	0	ID=annotation2172542;Name=BBa_M1528
BBa_M1533	.	CDS	3128	4561	.	+	0	ID=annotation2172518;Name=HlyD
BBa_M1533	.	start_codon	4593	4595	.	+	0	ID=annotation2172525;Name=Start
BBa_M1533	.	engineered_region	4805	5524	.	+	0	ID=annotation2172531;Name=BBa_K208000
BBa_M1533	.	engineered_region	441	947	.	+	0	ID=annotation2172510;Name=BBa_M1525
BBa_M1533	.	engineered_region	9150	9278	.	+	0	ID=annotation2172549;Name=BBa_B0015
BBa_M1533	.	stop_codon	3097	3099	.	+	0	ID=annotation2172515;Name=Stop Codon
BBa_M1533	.	stop_codon	9271	9271	.	+	0	ID=annotation2172548;Name=stop
BBa_M1533	.	start_codon	229	231	.	+	0	ID=annotation2172502;Name=Start
BBa_M1533	.	engineered_region	1	220	.	+	0	ID=annotation2172500;Name=BBa_K208010
BBa_M1533	.	engineered_region	4573	4584	.	+	0	ID=annotation2172523;Name=BBa_B0034
BBa_M1533	.	tag	229	414	.	+	0	ID=annotation2172501;Name=HlyA
BBa_M1533	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2172499;Name=BBa_B0034
BBa_M1533	.	start_codon	4805	4807	.	+	0	ID=annotation2172529;Name=Start
BBa_M1533	.	engineered_region	7685	7698	.	+	0	ID=annotation2172537;Name=BBa_B0035
BBa_M1533	.	stop_codon	945	947	.	+	0	ID=annotation2172509;Name=stop
BBa_M1533	.	start_codon	7705	7707	.	+	0	ID=annotation2172538;Name=Start Codon
BBa_M1533	.	engineered_region	4787	4798	.	+	0	ID=annotation2172528;Name=BBa_B0034
BBa_M1533	.	stop_codon	4562	4564	.	+	0	ID=annotation2172520;Name=Stop Codon
BBa_M1533	.	engineered_region	4593	4778	.	+	0	ID=annotation2172526;Name=BBa_K208006
BBa_M1533	.	engineered_region	229	414	.	+	0	ID=annotation2172503;Name=BBa_K208006
BBa_M1533	.	engineered_region	956	4564	.	+	0	ID=annotation2172521;Name=BBa_M1528
BBa_M1533	.	CDS	4805	5524	.	+	0	ID=annotation2172530;Name=GFP Coding Region
BBa_M1533	.	start_codon	441	443	.	+	0	ID=annotation2172506;Name=start
BBa_M1533	.	engineered_region	956	969	.	+	0	ID=annotation2172511;Name=BBa_B0035
BBa_M1533	.	conserved_region	427	430	.	+	0	ID=annotation2172504;Name=conserved
BBa_M1533	.	engineered_region	9150	9229	.	+	0	ID=annotation2172543;Name=BBa_B0010
BBa_M1533	.	stem_loop	9161	9204	.	+	0	ID=annotation2172544;Name=stem_loop
BBa_M1533	.	engineered_region	7705	9141	.	+	0	ID=annotation2172540;Name=BBa_M1004
BBa_M1533	.	engineered_region	9238	9278	.	+	0	ID=annotation2172545;Name=BBa_B0012
BBa_M1533	.	tag	4593	4778	.	+	0	ID=annotation2172524;Name=HlyA
BBa_M1533	.	start_codon	976	978	.	+	0	ID=annotation2172512;Name=Start Codon
BBa_M1533	.	tag	921	944	.	+	0	ID=annotation2172508;Name=tag
BBa_M1533	.	engineered_region	976	3099	.	+	0	ID=annotation2172514;Name=BBa_M1003
BBa_M1533	.	CDS	5553	7673	.	+	0	ID=annotation2172534;Name=HlyB
BBa_M1533	.	engineered_region	3108	3121	.	+	0	ID=annotation2172516;Name=BBa_B0035
BBa_M1533	.	start_codon	5553	5555	.	+	0	ID=annotation2172533;Name=Start Codon
BBa_M1533	.	start_codon	3128	3130	.	+	0	ID=annotation2172517;Name=Start Codon
BBa_M1533	.	conserved_region	4577	4580	.	+	0	ID=annotation2172522;Name=conserved
BBa_M1533	.	CDS	7705	9138	.	+	0	ID=annotation2172539;Name=HlyD
BBa_M1533	.	promoter	1	200	.	+	0	ID=annotation2172497;Name=BBa_R0010
BBa_M1533	.	engineered_region	423	434	.	+	0	ID=annotation2172505;Name=BBa_B0034
BBa_M1533	.	conserved_region	4791	4794	.	+	0	ID=annotation2172527;Name=conserved
BBa_M1533	.	stem_loop	9245	9264	.	+	0	ID=annotation2172546;Name=T7 TE
>BBa_M1533
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctat
ggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaag
aggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccct
gaccacatcagcataataatactagagaaagaggagaaatactagatgtcttttaaaattagcacctttaccaaaatct
ggctcattatcgcggtcatcgtcatgtgtctgtgcaatgaatacaactgtcagtgtacgggggccgcagattgcacatc
gtgcacggcagcatgcactggttgtggcaattgtccgaacgcgattacctgtaccggaagcaagaattgcgtgcgcgca
acaacttgtactgatagcgaaaactgcgttaaagcacatacttgtacaggatcgacaaattgcaacagagcgaccacct
gtactaacagcaaggggtgcttggaggccacgacgtgcactggttctactcattgtcatcgcgcaactacgtgcacaaa
tagcaaagattgttttgaagctaccacatgtactgggtcatcaaactgctataccgctaccacctgcactaatagcacg
aactgctataaagctactgcatgtacaaatagtacggggtgcccagggcatcatcatcaccatcatcaccatcaccagt
actagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctgg
cacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgac
cagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatc
agcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacc
tgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcat
tctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaag
taccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttc
aggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgt
tgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggt
gcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtg
ttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttat
cttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttgg
tccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctgg
tcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagct
ggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaa
accgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctt
tcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtat
ttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaac
ggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtcta
tcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgctt
ttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcag
gtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgt
ccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacac
catcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaac
ccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcaca
agatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgat
ggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtac
cagctgcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttc
ctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacg
aaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcat
tatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactg
accctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaat
ccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctct
gctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaa
ctgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttct
ctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccg
catcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggcc
atcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagc
tggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcct
ggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggcc
agcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcag
aaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcat
caacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaa
aacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatc
tgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgt
gatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggaga
aatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagca
ggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatg
gacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatggctagcaaagga
gaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtg
gagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatg
gccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgacttt
ttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtg
ctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacat
tctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaa
gctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattg
gcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcg
tgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactag
agattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaa
tatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagct
ggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcct
gccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatc
tttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctga
ttgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccg
tcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggtt
gtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgt
tcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaa
gctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgt
gaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttct
tcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgt
cttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaa
tctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcag
gttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgt
gatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaac
atgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctg
tgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcga
tattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaa
cagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttaca
ttccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtggg
cgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtg
gaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcg
taggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaa
aatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatc
tgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaa
aaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagct
gcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgct
gcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaac
gaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgg
gcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccct
gtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgta
cgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgc
aaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgcc
ggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctact
tggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatca
accgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgc
aaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggag
cagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggaca
aactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgt
catccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaacc
ctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacg
tcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacat
caacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtct
accggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatct
cctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata