BBa_M1533
type: ComponentDefinition
persistent identity: BBa M1533
display id: BBa_M1533
version: 1
was generated by: iGEM to SBOL conversion
description: Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP
created: 2012-04-17T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12290
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: See subparts
star: false
creator: Sarah Allred
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_K208010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K208006
sequence annotation: Start
sequence annotation: HlyA
sequence annotation: conserved
sequence annotation: BBa_M1525
sequence annotation: start
sequence annotation: cds
sequence annotation: tag
sequence annotation: stop
sequence annotation: BBa_B0035
sequence annotation: BBa_M1528
sequence annotation: BBa_M1003
sequence annotation: HlyB
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence annotation: HlyD
sequence annotation: BBa_M1004
sequence annotation: BBa_K208000
sequence annotation: GFP Coding Region
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2172500
display id: annotation2172500
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172500/range2172500/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1533/annotation2172500/range2172500/1
type: Range
persistent identity: range2172500
display id: range2172500
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2172497
display id: annotation2172497
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172497/range2172497/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1533/annotation2172497/range2172497/1
type: Range
persistent identity: range2172497
display id: range2172497
version: 1
top level: BBa_M1533
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172499
display id: annotation2172499
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172499/range2172499/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2172499
display id: range2172499
version: 1
top level: BBa_M1533
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172503
display id: annotation2172503
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172503/range2172503/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172503
display id: range2172503
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 229
end: 414
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172502
display id: annotation2172502
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172502/range2172502/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172502
display id: range2172502
version: 1
top level: BBa_M1533
direction: direction/forward
start: 229
end: 231
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2172501
display id: annotation2172501
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172501/range2172501/1
role: SO:0000324
role: feature/tag

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type: Range
persistent identity: range2172501
display id: range2172501
version: 1
top level: BBa_M1533
direction: direction/forward
start: 229
end: 414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172505
display id: annotation2172505
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172505/range2172505/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172505
display id: range2172505
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 423
end: 434
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172504
display id: annotation2172504
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172504/range2172504/1
role: feature/conserved
role: SO:0000330

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type: Range
persistent identity: range2172504
display id: range2172504
version: 1
top level: BBa_M1533
direction: direction/forward
start: 427
end: 430
orientation: inline

BBa_M1525
type: SequenceAnnotation
persistent identity: annotation2172510
display id: annotation2172510
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172510/range2172510/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172510
display id: range2172510
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 441
end: 947
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2172506
display id: annotation2172506
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172506/range2172506/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2172506
display id: range2172506
version: 1
top level: BBa_M1533
direction: direction/forward
start: 441
end: 443
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2172507
display id: annotation2172507
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172507/range2172507/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2172507
display id: range2172507
version: 1
top level: BBa_M1533
direction: direction/forward
start: 444
end: 920
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2172508
display id: annotation2172508
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172508/range2172508/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_M1533/annotation2172508/range2172508/1
type: Range
persistent identity: range2172508
display id: range2172508
version: 1
top level: BBa_M1533
direction: direction/forward
start: 921
end: 944
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2172509
display id: annotation2172509
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172509/range2172509/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2172509
display id: range2172509
version: 1
top level: BBa_M1533
direction: direction/forward
start: 945
end: 947
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172511
display id: annotation2172511
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172511/range2172511/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172511
display id: range2172511
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 956
end: 969
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172521
display id: annotation2172521
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172521/range2172521/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172521
display id: range2172521
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 956
end: 4564
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172514
display id: annotation2172514
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172514/range2172514/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1533/annotation2172514/range2172514/1
type: Range
persistent identity: range2172514
display id: range2172514
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 976
end: 3099
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2172513
display id: annotation2172513
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172513/range2172513/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2172513
display id: range2172513
version: 1
top level: BBa_M1533
direction: direction/forward
start: 976
end: 3096
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172512
display id: annotation2172512
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172512/range2172512/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2172512
display id: range2172512
version: 1
top level: BBa_M1533
direction: direction/forward
start: 976
end: 978
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172515
display id: annotation2172515
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172515/range2172515/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1533/annotation2172515/range2172515/1
type: Range
persistent identity: range2172515
display id: range2172515
version: 1
top level: BBa_M1533
direction: direction/forward
start: 3097
end: 3099
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172516
display id: annotation2172516
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172516/range2172516/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1533/annotation2172516/range2172516/1
type: Range
persistent identity: range2172516
display id: range2172516
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 3108
end: 3121
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2172518
display id: annotation2172518
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172518/range2172518/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1533/annotation2172518/range2172518/1
type: Range
persistent identity: range2172518
display id: range2172518
version: 1
top level: BBa_M1533
direction: direction/forward
start: 3128
end: 4561
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172517
display id: annotation2172517
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172517/range2172517/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1533/annotation2172517/range2172517/1
type: Range
persistent identity: range2172517
display id: range2172517
version: 1
top level: BBa_M1533
direction: direction/forward
start: 3128
end: 3130
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172519
display id: annotation2172519
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172519/range2172519/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172519
display id: range2172519
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 3128
end: 4564
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172520
display id: annotation2172520
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172520/range2172520/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1533/annotation2172520/range2172520/1
type: Range
persistent identity: range2172520
display id: range2172520
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4562
end: 4564
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172523
display id: annotation2172523
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172523/range2172523/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172523
display id: range2172523
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 4573
end: 4584
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172522
display id: annotation2172522
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172522/range2172522/1
role: feature/conserved
role: SO:0000330

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type: Range
persistent identity: range2172522
display id: range2172522
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4577
end: 4580
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2172524
display id: annotation2172524
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172524/range2172524/1
role: SO:0000324
role: feature/tag

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type: Range
persistent identity: range2172524
display id: range2172524
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4593
end: 4778
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172526
display id: annotation2172526
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172526/range2172526/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172526
display id: range2172526
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 4593
end: 4778
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172525
display id: annotation2172525
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172525/range2172525/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2172525
display id: range2172525
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4593
end: 4595
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172528
display id: annotation2172528
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172528/range2172528/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172528
display id: range2172528
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 4787
end: 4798
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2172527
display id: annotation2172527
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172527/range2172527/1
role: feature/conserved
role: SO:0000330

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type: Range
persistent identity: range2172527
display id: range2172527
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4791
end: 4794
orientation: inline

BBa_K208000
type: SequenceAnnotation
persistent identity: annotation2172531
display id: annotation2172531
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172531/range2172531/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172531
display id: range2172531
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 4805
end: 5524
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2172530
display id: annotation2172530
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172530/range2172530/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range2172530
display id: range2172530
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4805
end: 5524
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2172529
display id: annotation2172529
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172529/range2172529/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2172529
display id: range2172529
version: 1
top level: BBa_M1533
direction: direction/forward
start: 4805
end: 4807
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172542
display id: annotation2172542
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172542/range2172542/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2172542
display id: range2172542
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 5533
end: 9141
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172532
display id: annotation2172532
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172532/range2172532/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172532
display id: range2172532
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 5533
end: 5546
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172535
display id: annotation2172535
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172535/range2172535/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2172535
display id: range2172535
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 5553
end: 7676
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2172534
display id: annotation2172534
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172534/range2172534/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2172534
display id: range2172534
version: 1
top level: BBa_M1533
direction: direction/forward
start: 5553
end: 7673
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172533
display id: annotation2172533
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172533/range2172533/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1533/annotation2172533/range2172533/1
type: Range
persistent identity: range2172533
display id: range2172533
version: 1
top level: BBa_M1533
direction: direction/forward
start: 5553
end: 5555
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172536
display id: annotation2172536
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172536/range2172536/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1533/annotation2172536/range2172536/1
type: Range
persistent identity: range2172536
display id: range2172536
version: 1
top level: BBa_M1533
direction: direction/forward
start: 7674
end: 7676
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172537
display id: annotation2172537
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172537/range2172537/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1533/annotation2172537/range2172537/1
type: Range
persistent identity: range2172537
display id: range2172537
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 7685
end: 7698
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172540
display id: annotation2172540
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172540/range2172540/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1533/annotation2172540/range2172540/1
type: Range
persistent identity: range2172540
display id: range2172540
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 7705
end: 9141
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2172539
display id: annotation2172539
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172539/range2172539/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1533/annotation2172539/range2172539/1
type: Range
persistent identity: range2172539
display id: range2172539
version: 1
top level: BBa_M1533
direction: direction/forward
start: 7705
end: 9138
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2172538
display id: annotation2172538
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172538/range2172538/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1533/annotation2172538/range2172538/1
type: Range
persistent identity: range2172538
display id: range2172538
version: 1
top level: BBa_M1533
direction: direction/forward
start: 7705
end: 7707
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2172541
display id: annotation2172541
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172541/range2172541/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1533/annotation2172541/range2172541/1
type: Range
persistent identity: range2172541
display id: range2172541
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9139
end: 9141
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2172549
display id: annotation2172549
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172549/range2172549/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1533/annotation2172549/range2172549/1
type: Range
persistent identity: range2172549
display id: range2172549
version: 1
top level: BBa_M1533
direction: direction/reverse
start: 9150
end: 9278
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2172543
display id: annotation2172543
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172543/range2172543/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1533/annotation2172543/range2172543/1
type: Range
persistent identity: range2172543
display id: range2172543
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9150
end: 9229
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2172544
display id: annotation2172544
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172544/range2172544/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_M1533/annotation2172544/range2172544/1
type: Range
persistent identity: range2172544
display id: range2172544
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9161
end: 9204
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2172545
display id: annotation2172545
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172545/range2172545/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1533/annotation2172545/range2172545/1
type: Range
persistent identity: range2172545
display id: range2172545
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9238
end: 9278
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2172546
display id: annotation2172546
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172546/range2172546/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_M1533/annotation2172546/range2172546/1
type: Range
persistent identity: range2172546
display id: range2172546
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9245
end: 9264
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2172547
display id: annotation2172547
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172547/range2172547/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_M1533/annotation2172547/range2172547/1
type: Range
persistent identity: range2172547
display id: range2172547
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9265
end: 9278
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2172548
display id: annotation2172548
version: 1
top level: BBa_M1533
location: https://synbiohub.org/public/igem/BBa_M1533/annotation2172548/range2172548/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1533/annotation2172548/range2172548/1
type: Range
persistent identity: range2172548
display id: range2172548
version: 1
top level: BBa_M1533
direction: direction/forward
start: 9271
end: 9271
orientation: inline

https://synbiohub.org/public/igem/BBa_M1533_sequence/1
type: Sequence
persistent identity: BBa M1533 sequence
display id: BBa_M1533_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatgtcttttaaaattagcacctttaccaaaatctggctcattatcgcggtcatcgtcatgtgtctgtgcaatgaatacaactgtcagtgtacgggggccgcagattgcacatcgtgcacggcagcatgcactggttgtggcaattgtccgaacgcgattacctgtaccggaagcaagaattgcgtgcgcgcaacaacttgtactgatagcgaaaactgcgttaaagcacatacttgtacaggatcgacaaattgcaacagagcgaccacctgtactaacagcaaggggtgcttggaggccacgacgtgcactggttctactcattgtcatcgcgcaactacgtgcacaaatagcaaagattgttttgaagctaccacatgtactgggtcatcaaactgctataccgctaccacctgcactaatagcacgaactgctataaagctactgcatgtacaaatagtacggggtgcccagggcatcatcatcaccatcatcaccatcaccagtactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacac…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z