##gff-version 3
##sequence-region BBa_M1535 1 5626
BBa_M1535	.	tag	227	253	.	+	0	ID=BBa_M1534;Name=BBa_M1534
BBa_M1535	.	tag	230	253	.	+	0	ID=annotation2172667;Name=tag;Parent=BBa_M1534
BBa_M1535	.	start_codon	227	229	.	+	0	ID=annotation2172666;Name=start;Parent=BBa_M1534
BBa_M1535	.	tag	747	932	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1535	.	tag	747	932	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1535	.	start_codon	747	749	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1535	.	tag	5304	5489	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1535	.	tag	5304	5489	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1535	.	start_codon	5304	5306	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1535	.	engineered_region	941	4549	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1535	.	ribosome_entry_site	3093	3106	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1535	.	ribosome_entry_site	941	954	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1535	.	CDS	3113	4549	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1535	.	CDS	3113	4546	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1535	.	start_codon	3113	3115	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1535	.	stop_codon	4547	4549	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1535	.	CDS	961	3084	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1535	.	stop_codon	3082	3084	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1535	.	start_codon	961	963	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1535	.	CDS	961	3081	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1535	.	ribosome_entry_site	4558	4569	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1535	.	conserved_region	4562	4565	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1535	.	terminator	5498	5626	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1535	.	terminator	5498	5577	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1535	.	engineered_region	5498	5577	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1535	.	stem_loop	5509	5552	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1535	.	terminator	5586	5626	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1535	.	engineered_region	5586	5626	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1535	.	stop_codon	5619	5619	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1535	.	polyA_site	5613	5626	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1535	.	stem_loop	5593	5612	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1535	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1535	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1535	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1535	.	CDS	262	738	.	+	0	ID=BBa_M1524;Name=BBa_M1524
BBa_M1535	.	CDS	262	738	.	+	0	ID=annotation2172670;Name=cds;Parent=BBa_M1524
BBa_M1535	.	CDS	4576	5295	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1535	.	CDS	4576	5295	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1535	.	start_codon	4576	4578	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
>BBa_M1535
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcatcatca
ccatcatcaccatcactactagaggccttcaaaacctgtggtttttcaaaaaaatggttagtcatagcagtcattgtca
tgtgcctgtgtacggaatgttactgccagtgtacaggtggcgctgattgcacatcgtgtaccggggcatgtaccgggtg
tggcaattgtccgaacgcggttacatgcacaaactcacagcattgcgttaaggcaaacacgtgcacagattctgagaat
tgcgttaaagctcatacatgcaccggtagtaccgattgtaataccgcgcagacctgtactaacagcaaggattgcttcg
aagcgaatacttgcaccggcagtacgcattgccatcgagctactacgtgtaccaattcgaaagattgctttgaggcaac
cacatgcaccggctcctccaattgctataccgcgaccacctgcacagatagcactaattgttacaaagctacggcgtgc
acaaactcttctggctgcccggggcattactagagttagcctatggaagtcagggtgatcttaatccattaattaatga
aatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtcc
ggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagattaaaga
ggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacg
tctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggc
agcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactg
gtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctgg
agcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcg
ttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttc
atcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggaca
aagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcat
tctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgt
cacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatc
agatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaat
gtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctct
ccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactg
ctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgc
tgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcatt
aacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgg
gtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcct
gggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttc
cgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaag
tcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaa
cggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctg
caagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagtta
tctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaaca
gggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatc
ttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtc
gcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagat
cgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgat
taatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataag
ctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgc
cagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggt
tattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgt
tctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcg
atgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtct
ggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaacca
tacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaacc
agaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacga
gaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgct
gtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgaga
gcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtca
aaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgct
ccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggtta
ttgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaa
cgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggac
gctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaaca
aacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgct
gtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggagaaatactagatggcta
gcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaa
gacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagat
ggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatg
gaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaac
gaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaat
aatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagca
ggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatg
gacggaactcaataaccctgaccacatcagcataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata