LOCUS BBa_M1536 5626 bp DNA linear 22-Dec-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1536 VERSION BBa_M1536.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=start misc_feature 230..253 /label=tag misc_feature 227..253 /label=BBa_M1534 CDS 262..738 /label=cds CDS 262..738 /label=BBa_M1525 misc_feature 747..932 /label=HlyA misc_feature 747..749 /label=Start misc_feature 747..932 /label=BBa_K208006 RBS 941..954 /label=BBa_B0035 misc_feature 961..963 /label=Start Codon misc_feature 3082..3084 /label=Stop Codon CDS 961..3081 /label=HlyB CDS 961..3084 /label=BBa_M1003 RBS 3093..3106 /label=BBa_B0035 misc_feature 3113..3115 /label=Start Codon CDS 3113..4546 /label=HlyD misc_feature 4547..4549 /label=Stop Codon CDS 3113..4549 /label=BBa_M1004 misc_feature 941..4549 /label=BBa_M1528 misc_feature 4562..4565 /label=conserved RBS 4558..4569 /label=BBa_B0034 misc_feature 4576..4578 /label=Start CDS 4576..5295 /label=GFP Coding Region CDS 4576..5295 /label=BBa_K208000 misc_feature 5304..5489 /label=HlyA misc_feature 5304..5306 /label=Start misc_feature 5304..5489 /label=BBa_K208006 stem_loop 5509..5552 /label=stem_loop misc_feature 5498..5577 /label=BBa_B0010 terminator 5498..5577 /label=BBa_B0010 stem_loop 5593..5612 /label=T7 TE misc_feature 5619..5619 /label=stop polyA_site 5613..5626 /label=polya misc_feature 5586..5626 /label=BBa_B0012 terminator 5586..5626 /label=BBa_B0012 terminator 5498..5626 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcatcacca 241 tcatcaccat cactactaga gtcttttaaa attagcacct ttaccaaaat ctggctcatt 301 atcgcggtca tcgtcatgtg tctgtgcaat gaatacaact gtcagtgtac gggggccgca 361 gattgcacat cgtgcacggc agcatgcact ggttgtggca attgtccgaa cgcgattacc 421 tgtaccggaa gcaagaattg cgtgcgcgca acaacttgta ctgatagcga aaactgcgtt 481 aaagcacata cttgtacagg atcgacaaat tgcaacagag cgaccacctg tactaacagc 541 aaggggtgct tggaggccac gacgtgcact ggttctactc attgtcatcg cgcaactacg 601 tgcacaaata gcaaagattg ttttgaagct accacatgta ctgggtcatc aaactgctat 661 accgctacca cctgcactaa tagcacgaac tgctataaag ctactgcatg tacaaatagt 721 acggggtgcc cagggcatta ctagagttag cctatggaag tcagggtgat cttaatccat 781 taattaatga aatcagcaaa atcatttcag cagcaggtag cttcgatgtt aaagaggaaa 841 gaaccgcagc ttctttattg cagttgtccg gtaatgccag tgatttttca tatggacgga 901 actcaataac cctgaccaca tcagcataat aatactagag attaaagagg agaatactag 961 atggattcct gtcacaagat tgattacggc ctgtatgcac tggaaatcct ggcacaatat 1021 cataacgtct ccgtcaaccc ggaggaaatt aaacatcgct tcgatactga cggcactggc 1081 ctgggtctga ccagctggct gctggcagcg aagtccctgg agctgaaggt aaagcaagtg 1141 aaaaagacga ttgatcgtct gaacttcatc agcctgccgg cactggtttg gcgtgaagat 1201 ggccgtcact tcatcctgac gaaagtgtcc aaagaagcga accgctacct gatctttgat 1261 ctggagcagc gcaacccgcg cgtactggaa cagagcgagt tcgaggctct gtatcagggc 1321 cacatcattc tgattgcatc tcgttcttct gttgcaggta aactggcgaa attcgacttc 1381 acctggttca ttccggctat tatcaagtac cgtcgtattt tcatcgaaac cctggttgta 1441 tctgttttcc tgcaactgtt cgctctgatt actccgctgt tctttcaggt tgtcatggac 1501 aaagttctgg ttcaccgtgg cttttccacc ctgaacgtca ttactgttgc gctgagcgtt 1561 gttgttgtgt tcgaaatcat tctgtccggt ctgcgtacct acatcttcgc gcactctacc 1621 tcccgcatcg acgttgaact gggtgcgaag ctgttccgtc acctgctggc actgcctatc 1681 agctacttcg aatctcgtcg tgtgggcgat actgtggccc gtgttcgtga actggatcag 1741 atccgcaact ttctgaccgg ccaggccctg accagcgttc tggatctgct gttcagcttt 1801 atcttcttcg cagtaatgtg gtactacagc ccgaagctga ctctggtgat tctgttctct 1861 ctgccgtgtt acgcagcttg gtccgtcttc atctctccaa tcctgcgtcg ccgtctggac 1921 gacaaattta gccgtaatgc ggataatcag tccttcctgg tcgaatctgt gactgctatc 1981 aacactatca aagccatggc ggtttctcca cagatgacta acatctggga taagcagctg 2041 gcaggttacg ttgctgcggg cttcaaagtg accgttctgg caaccattgg tcaacagggc 2101 attcagctga ttcagaaaac cgtgatgatc attaacctgt ggctgggtgc gcacctggtc 2161 atcagcggtg acctgtccat tggccagctg atcgctttca acatgctggc gggtcaaatt 2221 gttgccccag taatccgtct ggcccagatc tggcaggact tccagcaggt cggtatttct 2281 gtgacccgcc tgggtgacgt cctgaactct ccgaccgaaa gctaccacgg taaactggct 2341 ctgccggaga tcaacggcga tattactttc cgtaacatcc gttttcgtta caaaccggat 2401 tccccggtaa tcctggataa catcaatctg tctatcaaac agggcgaagt catcggcatc 2461 gtgggccgta gcggcagcgg caagtccact ctgactaaac tgattcagcg cttttacatt 2521 ccagaaaacg gtcaggttct gattgacggt cacgacctgg ctctggcgga cccgaactgg 2581 ctgcgtcgtc aggtgggcgt agtgctgcaa gataacgtgc tgctgaaccg tagcatcatc 2641 gataacatca gcctggctaa cccgggcatg tccgtggaaa aagttatcta tgctgcgaaa 2701 ctggcaggcg cgcacgattt catctctgaa ctgcgtgaag gctacaacac catcgtaggc 2761 gaacagggcg ctggcctgtc cggcggtcaa cgtcagcgta tcgctattgc acgtgcactg 2821 gtgaacaacc cgaaaatcct gatcttcgac gaggccactt ctgctctgga ttacgaatct 2881 gaacatatta tcatgcgtaa tatgcacaag atctgcaaag gtcgcaccgt aatcatcatc 2941 gcccaccgtc tgagcaccgt aaagaacgcc gaccgcatca tcgtgatgga aaaaggtaag 3001 atcgttgaac agggtaaaca taaagagctg ctgtccgaac cggaatctct gtattcttat 3061 ctgtaccagc tgcaatctga ttaatactag agattaaaga ggagaatact agatgaaaac 3121 ttggctgatg ggcttctctg agttcctgct gcgttataag ctggtatgga gcgaaacttg 3181 gaaaatccgt aagcaactgg ataccccggt acgtgaaaaa gacgaaaacg aatttctgcc 3241 agcacatctg gaactgattg agacgccggt ttctcgccgt ccacgtctgg ttgcttactt 3301 cattatgggc ttcctggtta ttgctgtaat cctgtctgtt ctgggtcagg tggaaatcgt 3361 tgcgaccgcg aatggtaaac tgaccctgtc cggtcgttct aaagaaatca aaccgatcga 3421 aaactccatc gttaaagaga ttatcgttaa agaaggtgaa tccgtacgca aaggcgatgt 3481 tctgctgaaa ctgaccgcac tgggcgcaga agcggacact ctgaaaactc agtcctctct 3541 gctgcaaacc cgtctggaac agacccgtta ccaaatcctg agccgttcta ttgaactgaa 3601 caaactgccg gaactgaaac tgccggatga accatacttc cagaacgtaa gcgaggagga 3661 agttctgcgc ctgacttccc tgatcaaaga gcagttctct acttggcaga accagaaata 3721 ccaaaaggaa ctgaacctgg acaaaaagcg tgcggagcgc ctgactatcc tggcccgcat 3781 caaccgttac gagaacctgt ctcgtgttga aaaatctcgc ctggacgact tccgtagcct 3841 gctgcacaaa caggccatcg caaaacacgc tgtgctggaa caggaaaata aatacgtgga 3901 agctgccaac gaactgcgtg tatataaaag ccagctggag cagatcgaga gcgaaatcct 3961 gtctgcgaag gaggagtacc agctggtgac gcagctgttc aaaaatgaaa tcctggacaa 4021 actgcgtcaa accacggact ctatcgaact gctgaccctg gaactggaga aaaacgagga 4081 gcgccagcag gccagcgtca tccgcgctcc ggtatctggt aaagttcagc aactgaaagt 4141 tcacaccgaa ggtggcgttg ttaccaccgc agaaaccctg atggttattg tgccggaaga 4201 tgacaccctg gaagttaccg ctctggtgca gaacaaagat atcggtttca tcaacgtcgg 4261 tcagaacgcg atcatcaaag tagaggcttt cccgtacact cgttacggct acctggtggg 4321 caaggtcaaa aacatcaacc tggacgctat cgaagatcag aaactgggcc tggtgttcaa 4381 cgtgatcgtt agcgtagaag agaacgatct gtctaccggt aacaaacaca ttccactgtc 4441 ctccggcatg gcggttactg ctgaaatcaa aaccggtatg cgtagcgtga tctcctatct 4501 gctgtctccg ctggaagaaa gcgtcaccga atctctgcac gaacgctaat actagagaaa 4561 gaggagaaat actagatggc tagcaaagga gaagaacttt tcactggagt tgtcccaatt 4621 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 4681 ggtgatgcta catacggaaa gcttaccctt aaatttattt gcactactgg aaaactacct 4741 gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcccgttat 4801 ccggatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 4861 gaacgcacta tatctttcaa agatgacggg aactacaaga cgcgtgctga agtcaagttt 4921 gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta ttgattttaa agaagatgga 4981 aacattctcg gacacaaact cgagtacaac tataactcac acaatgtata catcacggca 5041 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacat tgaagatgga 5101 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 5161 ttaccagaca accattacct gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag 5221 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat 5281 gagctctaca aataatacta gagttagcct atggaagtca gggtgatctt aatccattaa 5341 ttaatgaaat cagcaaaatc atttcagcag caggtagctt cgatgttaaa gaggaaagaa 5401 ccgcagcttc tttattgcag ttgtccggta atgccagtga tttttcatat ggacggaact 5461 caataaccct gaccacatca gcataataat actagagcca ggcatcaaat aaaacgaaag 5521 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 5581 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //