LOCUS BBa_M1539 9254 bp DNA linear 26-Dec-2024 DEFINITION YL2 - antifreeze protein ACCESSION BBa_M1539 VERSION BBa_M1539.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 229..414 /label=HlyA misc_feature 229..231 /label=Start misc_feature 229..414 /label=BBa_K208006 misc_feature 427..430 /label=conserved RBS 423..434 /label=BBa_B0034 misc_feature 441..461 /label=start misc_feature 911..921 /label=stop CDS 441..923 /label=BBa_M1538 RBS 932..945 /label=BBa_B0035 misc_feature 952..954 /label=Start Codon misc_feature 3073..3075 /label=Stop Codon CDS 952..3072 /label=HlyB CDS 952..3075 /label=BBa_M1003 RBS 3084..3097 /label=BBa_B0035 misc_feature 3104..3106 /label=Start Codon CDS 3104..4537 /label=HlyD misc_feature 4538..4540 /label=Stop Codon CDS 3104..4540 /label=BBa_M1004 misc_feature 932..4540 /label=BBa_M1528 misc_feature 4553..4556 /label=conserved RBS 4549..4560 /label=BBa_B0034 misc_feature 4569..4754 /label=HlyA misc_feature 4569..4571 /label=Start misc_feature 4569..4754 /label=BBa_K208006 misc_feature 4767..4770 /label=conserved RBS 4763..4774 /label=BBa_B0034 misc_feature 4781..4783 /label=Start CDS 4781..5500 /label=GFP Coding Region CDS 4781..5500 /label=BBa_K208000 RBS 5509..5522 /label=BBa_B0035 misc_feature 5529..5531 /label=Start Codon misc_feature 7650..7652 /label=Stop Codon CDS 5529..7649 /label=HlyB CDS 5529..7652 /label=BBa_M1003 RBS 7661..7674 /label=BBa_B0035 misc_feature 7681..7683 /label=Start Codon CDS 7681..9114 /label=HlyD misc_feature 9115..9117 /label=Stop Codon CDS 7681..9117 /label=BBa_M1004 misc_feature 5509..9117 /label=BBa_M1528 stem_loop 9137..9180 /label=stem_loop misc_feature 9126..9205 /label=BBa_B0010 terminator 9126..9205 /label=BBa_B0010 stem_loop 9221..9240 /label=T7 TE misc_feature 9247..9247 /label=stop polyA_site 9241..9254 /label=polya misc_feature 9214..9254 /label=BBa_B0012 terminator 9214..9254 /label=BBa_B0012 terminator 9126..9254 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt agcctatgga 241 agtcagggtg atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt 301 agcttcgatg ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc 361 agtgattttt catatggacg gaactcaata accctgacca catcagcata ataatactag 421 agaaagagga gaaatactag atggctttca aaacgtgcgg attcagcaag aaatggttgg 481 ttattgccgt tatagtcatg tgcctttgta ctgaatgcta ttgtcactgt acgggtggag 541 ccgattgcac ctcctgtact gatgcttgca cgggttgtgg taattgccca aacgcgcata 601 cctgtactga cagcaagaat gtttgtaagg cggcaacctg tacagatagt gagaattgtg 661 tgaaagccca tacatgtacc ggcagtacga aatgtaacac ggcgagaaca tgtactaact 721 ctaaggactg ctttgaagcg aaaacgtgta ccgggagtac gcattgtcat cgtgcgacca 781 cttgcactaa ttcaaaagat tgtttcgaag ccaccacctg tacagggagt tcgaattgtt 841 atacagcgac cacgtgtaca gacagtacca attgctataa agctaccgcg tgtaccaatt 901 cgaccgggtg tccaggacat tagtactaga gattaaagag gagaatacta gatggattcc 961 tgtcacaaga ttgattacgg cctgtatgca ctggaaatcc tggcacaata tcataacgtc 1021 tccgtcaacc cggaggaaat taaacatcgc ttcgatactg acggcactgg cctgggtctg 1081 accagctggc tgctggcagc gaagtccctg gagctgaagg taaagcaagt gaaaaagacg 1141 attgatcgtc tgaacttcat cagcctgccg gcactggttt ggcgtgaaga tggccgtcac 1201 ttcatcctga cgaaagtgtc caaagaagcg aaccgctacc tgatctttga tctggagcag 1261 cgcaacccgc gcgtactgga acagagcgag ttcgaggctc tgtatcaggg ccacatcatt 1321 ctgattgcat ctcgttcttc tgttgcaggt aaactggcga aattcgactt cacctggttc 1381 attccggcta ttatcaagta ccgtcgtatt ttcatcgaaa ccctggttgt atctgttttc 1441 ctgcaactgt tcgctctgat tactccgctg ttctttcagg ttgtcatgga caaagttctg 1501 gttcaccgtg gcttttccac cctgaacgtc attactgttg cgctgagcgt tgttgttgtg 1561 ttcgaaatca ttctgtccgg tctgcgtacc tacatcttcg cgcactctac ctcccgcatc 1621 gacgttgaac tgggtgcgaa gctgttccgt cacctgctgg cactgcctat cagctacttc 1681 gaatctcgtc gtgtgggcga tactgtggcc cgtgttcgtg aactggatca gatccgcaac 1741 tttctgaccg gccaggccct gaccagcgtt ctggatctgc tgttcagctt tatcttcttc 1801 gcagtaatgt ggtactacag cccgaagctg actctggtga ttctgttctc tctgccgtgt 1861 tacgcagctt ggtccgtctt catctctcca atcctgcgtc gccgtctgga cgacaaattt 1921 agccgtaatg cggataatca gtccttcctg gtcgaatctg tgactgctat caacactatc 1981 aaagccatgg cggtttctcc acagatgact aacatctggg ataagcagct ggcaggttac 2041 gttgctgcgg gcttcaaagt gaccgttctg gcaaccattg gtcaacaggg cattcagctg 2101 attcagaaaa ccgtgatgat cattaacctg tggctgggtg cgcacctggt catcagcggt 2161 gacctgtcca ttggccagct gatcgctttc aacatgctgg cgggtcaaat tgttgcccca 2221 gtaatccgtc tggcccagat ctggcaggac ttccagcagg tcggtatttc tgtgacccgc 2281 ctgggtgacg tcctgaactc tccgaccgaa agctaccacg gtaaactggc tctgccggag 2341 atcaacggcg atattacttt ccgtaacatc cgttttcgtt acaaaccgga ttccccggta 2401 atcctggata acatcaatct gtctatcaaa cagggcgaag tcatcggcat cgtgggccgt 2461 agcggcagcg gcaagtccac tctgactaaa ctgattcagc gcttttacat tccagaaaac 2521 ggtcaggttc tgattgacgg tcacgacctg gctctggcgg acccgaactg gctgcgtcgt 2581 caggtgggcg tagtgctgca agataacgtg ctgctgaacc gtagcatcat cgataacatc 2641 agcctggcta acccgggcat gtccgtggaa aaagttatct atgctgcgaa actggcaggc 2701 gcgcacgatt tcatctctga actgcgtgaa ggctacaaca ccatcgtagg cgaacagggc 2761 gctggcctgt ccggcggtca acgtcagcgt atcgctattg cacgtgcact ggtgaacaac 2821 ccgaaaatcc tgatcttcga cgaggccact tctgctctgg attacgaatc tgaacatatt 2881 atcatgcgta atatgcacaa gatctgcaaa ggtcgcaccg taatcatcat cgcccaccgt 2941 ctgagcaccg taaagaacgc cgaccgcatc atcgtgatgg aaaaaggtaa gatcgttgaa 3001 cagggtaaac ataaagagct gctgtccgaa ccggaatctc tgtattctta tctgtaccag 3061 ctgcaatctg attaatacta gagattaaag aggagaatac tagatgaaaa cttggctgat 3121 gggcttctct gagttcctgc tgcgttataa gctggtatgg agcgaaactt ggaaaatccg 3181 taagcaactg gataccccgg tacgtgaaaa agacgaaaac gaatttctgc cagcacatct 3241 ggaactgatt gagacgccgg tttctcgccg tccacgtctg gttgcttact tcattatggg 3301 cttcctggtt attgctgtaa tcctgtctgt tctgggtcag gtggaaatcg ttgcgaccgc 3361 gaatggtaaa ctgaccctgt ccggtcgttc taaagaaatc aaaccgatcg aaaactccat 3421 cgttaaagag attatcgtta aagaaggtga atccgtacgc aaaggcgatg ttctgctgaa 3481 actgaccgca ctgggcgcag aagcggacac tctgaaaact cagtcctctc tgctgcaaac 3541 ccgtctggaa cagacccgtt accaaatcct gagccgttct attgaactga acaaactgcc 3601 ggaactgaaa ctgccggatg aaccatactt ccagaacgta agcgaggagg aagttctgcg 3661 cctgacttcc ctgatcaaag agcagttctc tacttggcag aaccagaaat accaaaagga 3721 actgaacctg gacaaaaagc gtgcggagcg cctgactatc ctggcccgca tcaaccgtta 3781 cgagaacctg tctcgtgttg aaaaatctcg cctggacgac ttccgtagcc tgctgcacaa 3841 acaggccatc gcaaaacacg ctgtgctgga acaggaaaat aaatacgtgg aagctgccaa 3901 cgaactgcgt gtatataaaa gccagctgga gcagatcgag agcgaaatcc tgtctgcgaa 3961 ggaggagtac cagctggtga cgcagctgtt caaaaatgaa atcctggaca aactgcgtca 4021 aaccacggac tctatcgaac tgctgaccct ggaactggag aaaaacgagg agcgccagca 4081 ggccagcgtc atccgcgctc cggtatctgg taaagttcag caactgaaag ttcacaccga 4141 aggtggcgtt gttaccaccg cagaaaccct gatggttatt gtgccggaag atgacaccct 4201 ggaagttacc gctctggtgc agaacaaaga tatcggtttc atcaacgtcg gtcagaacgc 4261 gatcatcaaa gtagaggctt tcccgtacac tcgttacggc tacctggtgg gcaaggtcaa 4321 aaacatcaac ctggacgcta tcgaagatca gaaactgggc ctggtgttca acgtgatcgt 4381 tagcgtagaa gagaacgatc tgtctaccgg taacaaacac attccactgt cctccggcat 4441 ggcggttact gctgaaatca aaaccggtat gcgtagcgtg atctcctatc tgctgtctcc 4501 gctggaagaa agcgtcaccg aatctctgca cgaacgctaa tactagagaa agaggagaaa 4561 tactagagtt agcctatgga agtcagggtg atcttaatcc attaattaat gaaatcagca 4621 aaatcatttc agcagcaggt agcttcgatg ttaaagagga aagaaccgca gcttctttat 4681 tgcagttgtc cggtaatgcc agtgattttt catatggacg gaactcaata accctgacca 4741 catcagcata ataatactag agaaagagga gaaatactag atggctagca aaggagaaga 4801 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 4861 attttctgtc agtggagagg gtgaaggtga tgctacatac ggaaagctta cccttaaatt 4921 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta 4981 tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact ttttcaagag 5041 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaacta 5101 caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa 5161 aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt acaactataa 5221 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa 5281 aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc aacaaaatac 5341 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgc 5401 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc 5461 tgctgggatt acacatggca tggatgagct ctacaaataa tactagagat taaagaggag 5521 aatactagat ggattcctgt cacaagattg attacggcct gtatgcactg gaaatcctgg 5581 cacaatatca taacgtctcc gtcaacccgg aggaaattaa acatcgcttc gatactgacg 5641 gcactggcct gggtctgacc agctggctgc tggcagcgaa gtccctggag ctgaaggtaa 5701 agcaagtgaa aaagacgatt gatcgtctga acttcatcag cctgccggca ctggtttggc 5761 gtgaagatgg ccgtcacttc atcctgacga aagtgtccaa agaagcgaac cgctacctga 5821 tctttgatct ggagcagcgc aacccgcgcg tactggaaca gagcgagttc gaggctctgt 5881 atcagggcca catcattctg attgcatctc gttcttctgt tgcaggtaaa ctggcgaaat 5941 tcgacttcac ctggttcatt ccggctatta tcaagtaccg tcgtattttc atcgaaaccc 6001 tggttgtatc tgttttcctg caactgttcg ctctgattac tccgctgttc tttcaggttg 6061 tcatggacaa agttctggtt caccgtggct tttccaccct gaacgtcatt actgttgcgc 6121 tgagcgttgt tgttgtgttc gaaatcattc tgtccggtct gcgtacctac atcttcgcgc 6181 actctacctc ccgcatcgac gttgaactgg gtgcgaagct gttccgtcac ctgctggcac 6241 tgcctatcag ctacttcgaa tctcgtcgtg tgggcgatac tgtggcccgt gttcgtgaac 6301 tggatcagat ccgcaacttt ctgaccggcc aggccctgac cagcgttctg gatctgctgt 6361 tcagctttat cttcttcgca gtaatgtggt actacagccc gaagctgact ctggtgattc 6421 tgttctctct gccgtgttac gcagcttggt ccgtcttcat ctctccaatc ctgcgtcgcc 6481 gtctggacga caaatttagc cgtaatgcgg ataatcagtc cttcctggtc gaatctgtga 6541 ctgctatcaa cactatcaaa gccatggcgg tttctccaca gatgactaac atctgggata 6601 agcagctggc aggttacgtt gctgcgggct tcaaagtgac cgttctggca accattggtc 6661 aacagggcat tcagctgatt cagaaaaccg tgatgatcat taacctgtgg ctgggtgcgc 6721 acctggtcat cagcggtgac ctgtccattg gccagctgat cgctttcaac atgctggcgg 6781 gtcaaattgt tgccccagta atccgtctgg cccagatctg gcaggacttc cagcaggtcg 6841 gtatttctgt gacccgcctg ggtgacgtcc tgaactctcc gaccgaaagc taccacggta 6901 aactggctct gccggagatc aacggcgata ttactttccg taacatccgt tttcgttaca 6961 aaccggattc cccggtaatc ctggataaca tcaatctgtc tatcaaacag ggcgaagtca 7021 tcggcatcgt gggccgtagc ggcagcggca agtccactct gactaaactg attcagcgct 7081 tttacattcc agaaaacggt caggttctga ttgacggtca cgacctggct ctggcggacc 7141 cgaactggct gcgtcgtcag gtgggcgtag tgctgcaaga taacgtgctg ctgaaccgta 7201 gcatcatcga taacatcagc ctggctaacc cgggcatgtc cgtggaaaaa gttatctatg 7261 ctgcgaaact ggcaggcgcg cacgatttca tctctgaact gcgtgaaggc tacaacacca 7321 tcgtaggcga acagggcgct ggcctgtccg gcggtcaacg tcagcgtatc gctattgcac 7381 gtgcactggt gaacaacccg aaaatcctga tcttcgacga ggccacttct gctctggatt 7441 acgaatctga acatattatc atgcgtaata tgcacaagat ctgcaaaggt cgcaccgtaa 7501 tcatcatcgc ccaccgtctg agcaccgtaa agaacgccga ccgcatcatc gtgatggaaa 7561 aaggtaagat cgttgaacag ggtaaacata aagagctgct gtccgaaccg gaatctctgt 7621 attcttatct gtaccagctg caatctgatt aatactagag attaaagagg agaatactag 7681 atgaaaactt ggctgatggg cttctctgag ttcctgctgc gttataagct ggtatggagc 7741 gaaacttgga aaatccgtaa gcaactggat accccggtac gtgaaaaaga cgaaaacgaa 7801 tttctgccag cacatctgga actgattgag acgccggttt ctcgccgtcc acgtctggtt 7861 gcttacttca ttatgggctt cctggttatt gctgtaatcc tgtctgttct gggtcaggtg 7921 gaaatcgttg cgaccgcgaa tggtaaactg accctgtccg gtcgttctaa agaaatcaaa 7981 ccgatcgaaa actccatcgt taaagagatt atcgttaaag aaggtgaatc cgtacgcaaa 8041 ggcgatgttc tgctgaaact gaccgcactg ggcgcagaag cggacactct gaaaactcag 8101 tcctctctgc tgcaaacccg tctggaacag acccgttacc aaatcctgag ccgttctatt 8161 gaactgaaca aactgccgga actgaaactg ccggatgaac catacttcca gaacgtaagc 8221 gaggaggaag ttctgcgcct gacttccctg atcaaagagc agttctctac ttggcagaac 8281 cagaaatacc aaaaggaact gaacctggac aaaaagcgtg cggagcgcct gactatcctg 8341 gcccgcatca accgttacga gaacctgtct cgtgttgaaa aatctcgcct ggacgacttc 8401 cgtagcctgc tgcacaaaca ggccatcgca aaacacgctg tgctggaaca ggaaaataaa 8461 tacgtggaag ctgccaacga actgcgtgta tataaaagcc agctggagca gatcgagagc 8521 gaaatcctgt ctgcgaagga ggagtaccag ctggtgacgc agctgttcaa aaatgaaatc 8581 ctggacaaac tgcgtcaaac cacggactct atcgaactgc tgaccctgga actggagaaa 8641 aacgaggagc gccagcaggc cagcgtcatc cgcgctccgg tatctggtaa agttcagcaa 8701 ctgaaagttc acaccgaagg tggcgttgtt accaccgcag aaaccctgat ggttattgtg 8761 ccggaagatg acaccctgga agttaccgct ctggtgcaga acaaagatat cggtttcatc 8821 aacgtcggtc agaacgcgat catcaaagta gaggctttcc cgtacactcg ttacggctac 8881 ctggtgggca aggtcaaaaa catcaacctg gacgctatcg aagatcagaa actgggcctg 8941 gtgttcaacg tgatcgttag cgtagaagag aacgatctgt ctaccggtaa caaacacatt 9001 ccactgtcct ccggcatggc ggttactgct gaaatcaaaa ccggtatgcg tagcgtgatc 9061 tcctatctgc tgtctccgct ggaagaaagc gtcaccgaat ctctgcacga acgctaatac 9121 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 9181 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 9241 tttctgcgtt tata //