##gff-version 3
##sequence-region BBa_M1541 1 5486
BBa_M1541	.	CDS	227	598	.	+	0	ID=BBa_M1521;Name=BBa_M1521
BBa_M1541	.	CDS	227	598	.	+	0	ID=annotation2172665;Name=Coding Sequence;Parent=BBa_M1521
BBa_M1541	.	start_codon	227	229	.	+	0	ID=annotation2172664;Name=Translation Start;Parent=BBa_M1521
BBa_M1541	.	tag	5164	5349	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1541	.	tag	5164	5349	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1541	.	start_codon	5164	5166	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1541	.	CDS	4436	5155	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1541	.	start_codon	4436	4438	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1541	.	CDS	4436	5155	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1541	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1541	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1541	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1541	.	terminator	5358	5486	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1541	.	terminator	5446	5486	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1541	.	polyA_site	5473	5486	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1541	.	stop_codon	5479	5479	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1541	.	stem_loop	5453	5472	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1541	.	engineered_region	5446	5486	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1541	.	terminator	5358	5437	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1541	.	stem_loop	5369	5412	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1541	.	engineered_region	5358	5437	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1541	.	ribosome_entry_site	4418	4429	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1541	.	conserved_region	4422	4425	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1541	.	tag	607	792	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1541	.	tag	607	792	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1541	.	start_codon	607	609	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1541	.	engineered_region	801	4409	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1541	.	ribosome_entry_site	2953	2966	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1541	.	ribosome_entry_site	801	814	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1541	.	CDS	2973	4409	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1541	.	CDS	2973	4406	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1541	.	start_codon	2973	2975	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1541	.	stop_codon	4407	4409	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1541	.	CDS	821	2944	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1541	.	stop_codon	2942	2944	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1541	.	CDS	821	2941	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1541	.	start_codon	821	823	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
>BBa_M1541
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcctttaa
aacatgcggatttagtaaaaaatggttaatcatagccgttattgtgatgtgcttatgtacagaatgctattgccagtgc
acaggaggtgctgattgtaccagttgcaccgctgcctgcacgggctgcggctcttgtcccaatgctcatacgtgtacag
attcaaaaaattgtgttcgtgcagaaacctgtacagattccgaaaattgcgtgaaagcccatacttgtacgggttcccg
caattgcaataccgcaatgacgtgtacaaactcgaaagactgctttgaagcaaaaacctgtaccgattccaccaattgt
tataaggcaaccgcgtgtactaattctaccgggtgtccaggacattactagagttagcctatggaagtcagggtgatct
taatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagct
tctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataat
aatactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcc
tggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtct
gaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttc
atcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgct
acctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacat
cattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatc
aagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttct
ttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgt
tgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactg
ggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggccc
gtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctt
tatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagct
tggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcc
tggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagca
gctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcag
aaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcg
ctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcgg
tatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatc
aacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgt
ctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcg
cttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgt
caggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggca
tgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaa
caccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaac
aacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgc
acaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgt
gatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctg
taccagctgcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgag
ttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaag
acgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttactt
cattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaa
ctgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtg
aatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctc
tctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactg
aaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagt
tctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggc
ccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacag
gccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagcc
agctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaat
cctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcag
gccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccg
cagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggttt
catcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtc
aaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacg
atctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtag
cgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagagg
agaaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgt
taatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgc
actactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatc
cggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaa
agatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggt
attgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacgg
cagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgcc
ctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggca
tggatgagctctacaaataatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagc
aaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatg
ccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata