LOCUS BBa_M1542 5519 bp DNA linear 26-Nov-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1542 VERSION BBa_M1542.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=start misc_feature 230..253 /label=tag misc_feature 227..253 /label=BBa_M1534 misc_feature 260..262 /label=Translation Start CDS 260..631 /label=Coding Sequence CDS 260..631 /label=BBa_M1521 misc_feature 640..825 /label=HlyA misc_feature 640..642 /label=Start misc_feature 640..825 /label=BBa_K208006 RBS 834..847 /label=BBa_B0035 misc_feature 854..856 /label=Start Codon misc_feature 2975..2977 /label=Stop Codon CDS 854..2974 /label=HlyB CDS 854..2977 /label=BBa_M1003 RBS 2986..2999 /label=BBa_B0035 misc_feature 3006..3008 /label=Start Codon CDS 3006..4439 /label=HlyD misc_feature 4440..4442 /label=Stop Codon CDS 3006..4442 /label=BBa_M1004 misc_feature 834..4442 /label=BBa_M1528 misc_feature 4455..4458 /label=conserved RBS 4451..4462 /label=BBa_B0034 misc_feature 4469..4471 /label=Start CDS 4469..5188 /label=GFP Coding Region CDS 4469..5188 /label=BBa_K208000 misc_feature 5197..5382 /label=HlyA misc_feature 5197..5199 /label=Start misc_feature 5197..5382 /label=BBa_K208006 stem_loop 5402..5445 /label=stem_loop misc_feature 5391..5470 /label=BBa_B0010 terminator 5391..5470 /label=BBa_B0010 stem_loop 5486..5505 /label=T7 TE misc_feature 5512..5512 /label=stop polyA_site 5506..5519 /label=polya misc_feature 5479..5519 /label=BBa_B0012 terminator 5479..5519 /label=BBa_B0012 terminator 5391..5519 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcatcacca 241 tcatcaccat cactactaga tggcctttaa aacatgcgga tttagtaaaa aatggttaat 301 catagccgtt attgtgatgt gcttatgtac agaatgctat tgccagtgca caggaggtgc 361 tgattgtacc agttgcaccg ctgcctgcac gggctgcggc tcttgtccca atgctcatac 421 gtgtacagat tcaaaaaatt gtgttcgtgc agaaacctgt acagattccg aaaattgcgt 481 gaaagcccat acttgtacgg gttcccgcaa ttgcaatacc gcaatgacgt gtacaaactc 541 gaaagactgc tttgaagcaa aaacctgtac cgattccacc aattgttata aggcaaccgc 601 gtgtactaat tctaccgggt gtccaggaca ttactagagt tagcctatgg aagtcagggt 661 gatcttaatc cattaattaa tgaaatcagc aaaatcattt cagcagcagg tagcttcgat 721 gttaaagagg aaagaaccgc agcttcttta ttgcagttgt ccggtaatgc cagtgatttt 781 tcatatggac ggaactcaat aaccctgacc acatcagcat aataatacta gagattaaag 841 aggagaatac tagatggatt cctgtcacaa gattgattac ggcctgtatg cactggaaat 901 cctggcacaa tatcataacg tctccgtcaa cccggaggaa attaaacatc gcttcgatac 961 tgacggcact ggcctgggtc tgaccagctg gctgctggca gcgaagtccc tggagctgaa 1021 ggtaaagcaa gtgaaaaaga cgattgatcg tctgaacttc atcagcctgc cggcactggt 1081 ttggcgtgaa gatggccgtc acttcatcct gacgaaagtg tccaaagaag cgaaccgcta 1141 cctgatcttt gatctggagc agcgcaaccc gcgcgtactg gaacagagcg agttcgaggc 1201 tctgtatcag ggccacatca ttctgattgc atctcgttct tctgttgcag gtaaactggc 1261 gaaattcgac ttcacctggt tcattccggc tattatcaag taccgtcgta ttttcatcga 1321 aaccctggtt gtatctgttt tcctgcaact gttcgctctg attactccgc tgttctttca 1381 ggttgtcatg gacaaagttc tggttcaccg tggcttttcc accctgaacg tcattactgt 1441 tgcgctgagc gttgttgttg tgttcgaaat cattctgtcc ggtctgcgta cctacatctt 1501 cgcgcactct acctcccgca tcgacgttga actgggtgcg aagctgttcc gtcacctgct 1561 ggcactgcct atcagctact tcgaatctcg tcgtgtgggc gatactgtgg cccgtgttcg 1621 tgaactggat cagatccgca actttctgac cggccaggcc ctgaccagcg ttctggatct 1681 gctgttcagc tttatcttct tcgcagtaat gtggtactac agcccgaagc tgactctggt 1741 gattctgttc tctctgccgt gttacgcagc ttggtccgtc ttcatctctc caatcctgcg 1801 tcgccgtctg gacgacaaat ttagccgtaa tgcggataat cagtccttcc tggtcgaatc 1861 tgtgactgct atcaacacta tcaaagccat ggcggtttct ccacagatga ctaacatctg 1921 ggataagcag ctggcaggtt acgttgctgc gggcttcaaa gtgaccgttc tggcaaccat 1981 tggtcaacag ggcattcagc tgattcagaa aaccgtgatg atcattaacc tgtggctggg 2041 tgcgcacctg gtcatcagcg gtgacctgtc cattggccag ctgatcgctt tcaacatgct 2101 ggcgggtcaa attgttgccc cagtaatccg tctggcccag atctggcagg acttccagca 2161 ggtcggtatt tctgtgaccc gcctgggtga cgtcctgaac tctccgaccg aaagctacca 2221 cggtaaactg gctctgccgg agatcaacgg cgatattact ttccgtaaca tccgttttcg 2281 ttacaaaccg gattccccgg taatcctgga taacatcaat ctgtctatca aacagggcga 2341 agtcatcggc atcgtgggcc gtagcggcag cggcaagtcc actctgacta aactgattca 2401 gcgcttttac attccagaaa acggtcaggt tctgattgac ggtcacgacc tggctctggc 2461 ggacccgaac tggctgcgtc gtcaggtggg cgtagtgctg caagataacg tgctgctgaa 2521 ccgtagcatc atcgataaca tcagcctggc taacccgggc atgtccgtgg aaaaagttat 2581 ctatgctgcg aaactggcag gcgcgcacga tttcatctct gaactgcgtg aaggctacaa 2641 caccatcgta ggcgaacagg gcgctggcct gtccggcggt caacgtcagc gtatcgctat 2701 tgcacgtgca ctggtgaaca acccgaaaat cctgatcttc gacgaggcca cttctgctct 2761 ggattacgaa tctgaacata ttatcatgcg taatatgcac aagatctgca aaggtcgcac 2821 cgtaatcatc atcgcccacc gtctgagcac cgtaaagaac gccgaccgca tcatcgtgat 2881 ggaaaaaggt aagatcgttg aacagggtaa acataaagag ctgctgtccg aaccggaatc 2941 tctgtattct tatctgtacc agctgcaatc tgattaatac tagagattaa agaggagaat 3001 actagatgaa aacttggctg atgggcttct ctgagttcct gctgcgttat aagctggtat 3061 ggagcgaaac ttggaaaatc cgtaagcaac tggatacccc ggtacgtgaa aaagacgaaa 3121 acgaatttct gccagcacat ctggaactga ttgagacgcc ggtttctcgc cgtccacgtc 3181 tggttgctta cttcattatg ggcttcctgg ttattgctgt aatcctgtct gttctgggtc 3241 aggtggaaat cgttgcgacc gcgaatggta aactgaccct gtccggtcgt tctaaagaaa 3301 tcaaaccgat cgaaaactcc atcgttaaag agattatcgt taaagaaggt gaatccgtac 3361 gcaaaggcga tgttctgctg aaactgaccg cactgggcgc agaagcggac actctgaaaa 3421 ctcagtcctc tctgctgcaa acccgtctgg aacagacccg ttaccaaatc ctgagccgtt 3481 ctattgaact gaacaaactg ccggaactga aactgccgga tgaaccatac ttccagaacg 3541 taagcgagga ggaagttctg cgcctgactt ccctgatcaa agagcagttc tctacttggc 3601 agaaccagaa ataccaaaag gaactgaacc tggacaaaaa gcgtgcggag cgcctgacta 3661 tcctggcccg catcaaccgt tacgagaacc tgtctcgtgt tgaaaaatct cgcctggacg 3721 acttccgtag cctgctgcac aaacaggcca tcgcaaaaca cgctgtgctg gaacaggaaa 3781 ataaatacgt ggaagctgcc aacgaactgc gtgtatataa aagccagctg gagcagatcg 3841 agagcgaaat cctgtctgcg aaggaggagt accagctggt gacgcagctg ttcaaaaatg 3901 aaatcctgga caaactgcgt caaaccacgg actctatcga actgctgacc ctggaactgg 3961 agaaaaacga ggagcgccag caggccagcg tcatccgcgc tccggtatct ggtaaagttc 4021 agcaactgaa agttcacacc gaaggtggcg ttgttaccac cgcagaaacc ctgatggtta 4081 ttgtgccgga agatgacacc ctggaagtta ccgctctggt gcagaacaaa gatatcggtt 4141 tcatcaacgt cggtcagaac gcgatcatca aagtagaggc tttcccgtac actcgttacg 4201 gctacctggt gggcaaggtc aaaaacatca acctggacgc tatcgaagat cagaaactgg 4261 gcctggtgtt caacgtgatc gttagcgtag aagagaacga tctgtctacc ggtaacaaac 4321 acattccact gtcctccggc atggcggtta ctgctgaaat caaaaccggt atgcgtagcg 4381 tgatctccta tctgctgtct ccgctggaag aaagcgtcac cgaatctctg cacgaacgct 4441 aatactagag aaagaggaga aatactagat ggctagcaaa ggagaagaac ttttcactgg 4501 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 4561 tggagagggt gaaggtgatg ctacatacgg aaagcttacc cttaaattta tttgcactac 4621 tggaaaacta cctgttccat ggccaacact tgtcactact ttctcttatg gtgttcaatg 4681 cttttcccgt tatccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga 4741 aggttatgta caggaacgca ctatatcttt caaagatgac gggaactaca agacgcgtgc 4801 tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaaag gtattgattt 4861 taaagaagat ggaaacattc tcggacacaa actcgagtac aactataact cacacaatgt 4921 atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa 4981 cattgaagat ggatccgttc aactagcaga ccattatcaa caaaatactc caattggcga 5041 tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgccc tttcgaaaga 5101 tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac 5161 acatggcatg gatgagctct acaaataata ctagagttag cctatggaag tcagggtgat 5221 cttaatccat taattaatga aatcagcaaa atcatttcag cagcaggtag cttcgatgtt 5281 aaagaggaaa gaaccgcagc ttctttattg cagttgtccg gtaatgccag tgatttttca 5341 tatggacgga actcaataac cctgaccaca tcagcataat aatactagag ccaggcatca 5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //