##gff-version 3
##sequence-region BBa_M1542 1 5519
BBa_M1542	.	tag	227	253	.	+	0	ID=BBa_M1534;Name=BBa_M1534
BBa_M1542	.	start_codon	227	229	.	+	0	ID=annotation2172666;Name=start;Parent=BBa_M1534
BBa_M1542	.	tag	230	253	.	+	0	ID=annotation2172667;Name=tag;Parent=BBa_M1534
BBa_M1542	.	tag	640	825	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1542	.	start_codon	640	642	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1542	.	tag	640	825	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1542	.	engineered_region	834	4442	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1542	.	ribosome_entry_site	834	847	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1542	.	ribosome_entry_site	2986	2999	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1542	.	CDS	854	2977	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1542	.	start_codon	854	856	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1542	.	CDS	854	2974	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1542	.	stop_codon	2975	2977	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1542	.	CDS	3006	4442	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1542	.	CDS	3006	4439	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1542	.	start_codon	3006	3008	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1542	.	stop_codon	4440	4442	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1542	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1542	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1542	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1542	.	tag	5197	5382	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1542	.	start_codon	5197	5199	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1542	.	tag	5197	5382	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1542	.	CDS	260	631	.	+	0	ID=BBa_M1521;Name=BBa_M1521
BBa_M1542	.	start_codon	260	262	.	+	0	ID=annotation2172664;Name=Translation Start;Parent=BBa_M1521
BBa_M1542	.	CDS	260	631	.	+	0	ID=annotation2172665;Name=Coding Sequence;Parent=BBa_M1521
BBa_M1542	.	CDS	4469	5188	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1542	.	CDS	4469	5188	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1542	.	start_codon	4469	4471	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1542	.	ribosome_entry_site	4451	4462	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1542	.	conserved_region	4455	4458	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1542	.	terminator	5391	5519	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1542	.	terminator	5391	5470	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1542	.	stem_loop	5402	5445	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1542	.	engineered_region	5391	5470	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1542	.	terminator	5479	5519	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1542	.	stop_codon	5512	5512	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1542	.	engineered_region	5479	5519	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1542	.	stem_loop	5486	5505	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1542	.	polyA_site	5506	5519	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_M1542
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcatcatca
ccatcatcaccatcactactagatggcctttaaaacatgcggatttagtaaaaaatggttaatcatagccgttattgtg
atgtgcttatgtacagaatgctattgccagtgcacaggaggtgctgattgtaccagttgcaccgctgcctgcacgggct
gcggctcttgtcccaatgctcatacgtgtacagattcaaaaaattgtgttcgtgcagaaacctgtacagattccgaaaa
ttgcgtgaaagcccatacttgtacgggttcccgcaattgcaataccgcaatgacgtgtacaaactcgaaagactgcttt
gaagcaaaaacctgtaccgattccaccaattgttataaggcaaccgcgtgtactaattctaccgggtgtccaggacatt
actagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggt
agcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggac
ggaactcaataaccctgaccacatcagcataataatactagagattaaagaggagaatactagatggattcctgtcaca
agattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaaca
tcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaag
caagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttca
tcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaaca
gagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaa
ttcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcc
tgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccac
cctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttc
gcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctact
tcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggc
cctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctg
gtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgaca
aatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggt
ttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggca
accattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtca
tcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtct
ggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaa
agctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccgg
attccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcag
cggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgac
ctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagca
tcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgca
cgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgt
cagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggatt
acgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtct
gagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctg
ctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagattaaagaggagaatac
tagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatcc
gtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgcc
ggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggt
caggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaact
ccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactggg
cgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagc
cgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaag
ttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctgga
caaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgc
ctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtgg
aagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagta
ccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgacc
ctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactga
aagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagt
taccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccg
tacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctgg
tgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggc
ggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaa
tctctgcacgaacgctaatactagagaaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgct
acatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccga
aggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggt
gatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagt
acaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgcca
caacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttta
ccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttg
agtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagttagcctatggaagtcag
ggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaa
ccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatc
agcataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata