BBa_M1600
1
BBa_M1600
CXCR3
2012-03-26T11:00:00Z
2015-05-08T01:13:57Z
Aspergillus oryzae
putative type III polyketide synthase (PKS) gene
false
false
_768_
0
12289
9
Not in stock
false
na
false
Yoshitaro Heshiki
annotation2171440
1
Stop
range2171440
1
1099
1101
annotation2171439
1
Coding Sequence
range2171439
1
1
1101
annotation2171438
1
Start
range2171438
1
1
3
BBa_K136046
1
BBa_K136046
envZ*
2008-10-24T11:00:00Z
2015-05-08T01:10:03Z
bacterial genome of mutated (I281S) strain for EnvZ.
EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
false
false
_211_
0
3215
9
It's complicated
false
PCR and ligation in pCR4Blunt-TOPO
false
alexandra bouaziz
annotation1994303
1
TAA
range1994303
1
1351
1353
annotation1994304
1
ATG
range1994304
1
1
3
annotation1994273
1
I281S T->G
range1994273
1
842
842
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_M1603
1
BBa_M1603
CXCR + transmembrane + GFP
2012-04-22T11:00:00Z
2015-05-08T01:13:57Z
Various
CXCR + transmembrane + GFP
false
false
_768_
0
12289
9
Not in stock
false
no
false
Yoshitaro Heshiki
component2173075
1
BBa_B0012
component2173072
1
BBa_M1600
component2173100
1
BBa_B0012
component2173102
1
BBa_J54103
component2173105
1
BBa_B0012
component2173058
1
BBa_J23100
component2173073
1
BBa_B0010
component2173065
1
BBa_K136046
component2173103
1
BBa_B0010
component2173067
1
BBa_B0030
component2173086
1
BBa_B0030
component2173060
1
BBa_B0030
component2173098
1
BBa_B0010
component2173084
1
BBa_K121011
component2173095
1
BBa_B0030
annotation2173060
1
BBa_B0030
range2173060
1
44
58
annotation2173095
1
BBa_B0030
range2173095
1
3004
3019
annotation2173100
1
BBa_B0012
range2173100
1
3842
3882
annotation2173098
1
BBa_B0010
range2173098
1
3755
3834
annotation2173058
1
BBa_J23100
range2173058
1
1
35
annotation2173103
1
BBa_B0010
range2173103
1
3891
3970
annotation2173075
1
BBa_B0012
range2173075
1
2644
2684
annotation2173086
1
BBa_B0030
range2173086
1
2933
2947
annotation2173105
1
BBa_B0012
range2173105
1
3979
4019
annotation2173102
1
BBa_J54103
range2173102
1
2956
3882
annotation2173072
1
BBa_M1600
range2173072
1
1447
2547
annotation2173065
1
BBa_K136046
range2173065
1
65
1417
annotation2173073
1
BBa_B0010
range2173073
1
2556
2635
annotation2173084
1
BBa_K121011
range2173084
1
2693
2924
annotation2173067
1
BBa_B0030
range2173067
1
1426
1440
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K121011
1
BBa_K121011
promoter (lacI regulated)
2008-10-15T11:00:00Z
2015-05-08T01:09:41Z
na
pressure inducible lac promoter
false
false
_220_
0
3180
9
Not in stock
false
na
false
Masashi Kajita
annotation1981858
1
K121011
range1981858
1
1
232
annotation1981861
1
-10
range1981861
1
165
168
annotation1981859
1
CAP binding site
range1981859
1
94
131
annotation1981860
1
-35
range1981860
1
141
146
annotation1981862
1
LacI binding site
range1981862
1
169
203
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_J54103
1
BBa_J54103
Promoterless-GFP(A)
2006-10-16T11:00:00Z
2015-08-31T03:48:11Z
synthesis
Released HQ 2013
promoterless
false
true
_76_
0
762
76
In stock
true
as negative control
true
Yusaku Nakashima
annotation2152494
1
MulI
range2152494
1
41
46
annotation2152491
1
BamHI
range2152491
1
15
20
annotation2152502
1
T7 TE
range2152502
1
899
918
annotation2152495
1
RBS-1\Strong
range2152495
1
49
64
annotation2152497
1
RBS
range2152497
1
56
62
annotation2152490
1
SalI
range2152490
1
1
6
annotation2152496
1
BBa_B0030
range2152496
1
49
64
annotation2152500
1
BBa_B0012
range2152500
1
887
927
annotation2152501
1
stem_loop
range2152501
1
811
854
annotation2152498
1
BBa_E0040
range2152498
1
71
790
annotation2152492
1
BBa_J54025
range2152492
1
27
46
annotation2152493
1
BglII
range2152493
1
27
32
annotation2152499
1
BBa_B0010
range2152499
1
800
879
annotation2152489
1
BBa_J54033
range2152489
1
1
20
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_M1600_sequence
1
atggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaatataattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatggcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttgtcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctgggcgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcattgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactggggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggcatcgtattcaggcttatag
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M1603_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagattaaagaggagaaatactagatggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaatataattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatggcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttgtcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctgggcgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcattgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactggggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggcatcgtattcaggcttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcctactagagattaaagaggagaaatactagaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K121011_sequence
1
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K136046_sequence
1
atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaa
BBa_J54103_sequence
1
gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z