##gff-version 3
##sequence-region BBa_M1603 1 4019
BBa_M1603	.	terminator	3842	3882	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1603	.	engineered_region	3842	3882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1603	.	stop_codon	3875	3875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1603	.	stem_loop	3849	3868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1603	.	polyA_site	3869	3882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1603	.	terminator	3755	3834	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1603	.	stem_loop	3766	3809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1603	.	engineered_region	3755	3834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1603	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1603	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1603	.	terminator	2644	2684	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1603	.	engineered_region	2644	2684	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1603	.	stop_codon	2677	2677	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1603	.	stem_loop	2651	2670	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1603	.	polyA_site	2671	2684	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1603	.	ribosome_entry_site	2933	2947	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1603	.	engineered_region	2933	2947	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	2940	2944	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	2933	2947	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1603	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_M1603	.	terminator	3979	4019	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1603	.	engineered_region	3979	4019	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1603	.	stop_codon	4012	4012	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1603	.	stem_loop	3986	4005	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1603	.	polyA_site	4006	4019	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1603	.	promoter	2693	2924	.	+	0	ID=BBa_K121011;Name=BBa_K121011
BBa_M1603	.	engineered_region	2693	2924	.	+	0	ID=annotation1981858;Name=K121011;Parent=BBa_K121011
BBa_M1603	.	promoter	2833	2838	.	+	0	ID=annotation1981860;Name=-35;Parent=BBa_K121011
BBa_M1603	.	promoter	2857	2860	.	+	0	ID=annotation1981861;Name=-10;Parent=BBa_K121011
BBa_M1603	.	non_covalent_binding_site	2786	2823	.	+	0	ID=annotation1981859;Name=CAP binding site;Parent=BBa_K121011
BBa_M1603	.	non_covalent_binding_site	2861	2895	.	+	0	ID=annotation1981862;Name=LacI binding site;Parent=BBa_K121011
BBa_M1603	.	engineered_region	2956	3882	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_M1603	.	tag	2996	3001	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_M1603	.	tag	2956	2961	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
BBa_M1603	.	engineered_region	3004	3019	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_M1603	.	engineered_region	2982	3001	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_M1603	.	ribosome_entry_site	3004	3019	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_M1603	.	ribosome_entry_site	3011	3017	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_M1603	.	stem_loop	3766	3809	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_M1603	.	tag	2970	2975	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_M1603	.	engineered_region	3842	3882	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_M1603	.	engineered_region	3755	3834	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_M1603	.	stem_loop	3854	3873	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_M1603	.	tag	2982	2987	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_M1603	.	engineered_region	3026	3745	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_M1603	.	engineered_region	2956	2975	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_M1603	.	terminator	3891	3970	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1603	.	stem_loop	3902	3945	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1603	.	engineered_region	3891	3970	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1603	.	CDS	1447	2547	.	+	0	ID=BBa_M1600;Name=BBa_M1600
BBa_M1603	.	CDS	1447	2547	.	+	0	ID=annotation2171439;Name=Coding Sequence;Parent=BBa_M1600
BBa_M1603	.	stop_codon	2545	2547	.	+	0	ID=annotation2171440;Name=Stop;Parent=BBa_M1600
BBa_M1603	.	start_codon	1447	1449	.	+	0	ID=annotation2171438;Name=Start;Parent=BBa_M1600
BBa_M1603	.	ribosome_entry_site	1426	1440	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1603	.	engineered_region	1426	1440	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	1433	1437	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	1426	1440	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1603	.	CDS	65	1417	.	+	0	ID=BBa_K136046;Name=BBa_K136046
BBa_M1603	.	stop_codon	1415	1417	.	+	0	ID=annotation1994303;Name=TAA;Parent=BBa_K136046
BBa_M1603	.	point_mutation	906	906	.	+	0	ID=annotation1994273;Name=I281S T->G;Parent=BBa_K136046
BBa_M1603	.	start_codon	65	67	.	+	0	ID=annotation1994304;Name=ATG;Parent=BBa_K136046
BBa_M1603	.	terminator	2556	2635	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1603	.	stem_loop	2567	2610	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1603	.	engineered_region	2556	2635	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1603	.	ribosome_entry_site	3004	3019	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1603	.	engineered_region	3004	3018	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	3011	3015	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1603	.	ribosome_entry_site	3004	3018	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_M1603
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgc
ttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatc
tggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgat
gaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctg
gggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatc
agatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaac
ctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctat
acgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcg
aacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgt
tacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagt
cacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaat
cgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgcc
gatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaacc
gcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacg
ccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatga
cggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagc
ggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagc
ggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatacta
gagattaaagaggagaaatactagatggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgc
tgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggattt
cagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgct
gtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagata
ccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagt
tgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaac
attgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgt
gtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaata
taattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatg
gcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttg
tcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctggg
cgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcat
tgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactgg
ggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggc
atcgtattcaggcttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagaggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttccc
gactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacttta
tgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgc
ctactagagattaaagaggagaaatactagaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtg
agattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattaga
tggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaa
tttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttg
cgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactat
atttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgag
ttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtat
acatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttca
actagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccaca
caatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatta
cacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata