BBa_M1603
type: ComponentDefinition
persistent identity: BBa M1603
display id: BBa_M1603
version: 1
was generated by: iGEM to SBOL conversion
description: CXCR + transmembrane + GFP
created: 2012-04-22T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Various
owned by: james
owned by: myers
mutable description: CXCR + transmembrane + GFP
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12289
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no
star: false
creator: Yoshitaro Heshiki
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K136046
component: BBa_B0030
component: BBa_M1600
component: BBa_B0012
component: BBa_B0010
component: BBa_K121011
component: BBa_J54103
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence annotation: BBa_K136046
sequence annotation: BBa_M1600
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K121011
sequence annotation: BBa_J54103
sequence: https://synbiohub.org/public/igem/BBa_M1603_sequence/1

BBa_K136046
type: Component
persistent identity: component2173065
display id: component2173065
version: 1
top level: BBa_M1603
definition: BBa_K136046
access: public

BBa_B0030
type: Component
persistent identity: component2173060
display id: component2173060
version: 1
top level: BBa_M1603
definition: BBa_B0030
access: public

BBa_M1600
type: Component
persistent identity: component2173072
display id: component2173072
version: 1
top level: BBa_M1603
definition: BBa_M1600
access: public

BBa_B0030
type: Component
persistent identity: component2173067
display id: component2173067
version: 1
top level: BBa_M1603
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component2173075
display id: component2173075
version: 1
top level: BBa_M1603
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2173073
display id: component2173073
version: 1
top level: BBa_M1603
definition: BBa_B0010
access: public

BBa_B0030
type: Component
persistent identity: component2173086
display id: component2173086
version: 1
top level: BBa_M1603
definition: BBa_B0030
access: public

BBa_K121011
type: Component
persistent identity: component2173084
display id: component2173084
version: 1
top level: BBa_M1603
definition: BBa_K121011
access: public

BBa_B0010
type: Component
persistent identity: component2173098
display id: component2173098
version: 1
top level: BBa_M1603
definition: BBa_B0010
access: public

BBa_B0030
type: Component
persistent identity: component2173095
display id: component2173095
version: 1
top level: BBa_M1603
definition: BBa_B0030
access: public

BBa_J54103
type: Component
persistent identity: component2173102
display id: component2173102
version: 1
top level: BBa_M1603
definition: BBa_J54103
access: public

BBa_B0012
type: Component
persistent identity: component2173100
display id: component2173100
version: 1
top level: BBa_M1603
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component2173105
display id: component2173105
version: 1
top level: BBa_M1603
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2173103
display id: component2173103
version: 1
top level: BBa_M1603
definition: BBa_B0010
access: public

BBa_J23100
type: Component
persistent identity: component2173058
display id: component2173058
version: 1
top level: BBa_M1603
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2173058
display id: annotation2173058
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173058/range2173058/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_M1603/annotation2173058/range2173058/1
type: Range
persistent identity: range2173058
display id: range2173058
version: 1
top level: BBa_M1603
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2173060
display id: annotation2173060
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173060/range2173060/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1603/annotation2173060/range2173060/1
type: Range
persistent identity: range2173060
display id: range2173060
version: 1
top level: BBa_M1603
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K136046
type: SequenceAnnotation
persistent identity: annotation2173065
display id: annotation2173065
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173065/range2173065/1
component: BBa_K136046

https://synbiohub.org/public/igem/BBa_M1603/annotation2173065/range2173065/1
type: Range
persistent identity: range2173065
display id: range2173065
version: 1
top level: BBa_M1603
direction: direction/reverse
start: 65
end: 1417
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2173067
display id: annotation2173067
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173067/range2173067/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1603/annotation2173067/range2173067/1
type: Range
persistent identity: range2173067
display id: range2173067
version: 1
top level: BBa_M1603
direction: direction/forward
start: 1426
end: 1440
orientation: inline

BBa_M1600
type: SequenceAnnotation
persistent identity: annotation2173072
display id: annotation2173072
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173072/range2173072/1
component: BBa_M1600

https://synbiohub.org/public/igem/BBa_M1603/annotation2173072/range2173072/1
type: Range
persistent identity: range2173072
display id: range2173072
version: 1
top level: BBa_M1603
direction: direction/reverse
start: 1447
end: 2547
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2173073
display id: annotation2173073
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173073/range2173073/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1603/annotation2173073/range2173073/1
type: Range
persistent identity: range2173073
display id: range2173073
version: 1
top level: BBa_M1603
direction: direction/forward
start: 2556
end: 2635
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2173075
display id: annotation2173075
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173075/range2173075/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1603/annotation2173075/range2173075/1
type: Range
persistent identity: range2173075
display id: range2173075
version: 1
top level: BBa_M1603
direction: direction/forward
start: 2644
end: 2684
orientation: inline

BBa_K121011
type: SequenceAnnotation
persistent identity: annotation2173084
display id: annotation2173084
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173084/range2173084/1
component: BBa_K121011

https://synbiohub.org/public/igem/BBa_M1603/annotation2173084/range2173084/1
type: Range
persistent identity: range2173084
display id: range2173084
version: 1
top level: BBa_M1603
direction: direction/reverse
start: 2693
end: 2924
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2173086
display id: annotation2173086
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173086/range2173086/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1603/annotation2173086/range2173086/1
type: Range
persistent identity: range2173086
display id: range2173086
version: 1
top level: BBa_M1603
direction: direction/forward
start: 2933
end: 2947
orientation: inline

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation2173102
display id: annotation2173102
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173102/range2173102/1
component: BBa_J54103

https://synbiohub.org/public/igem/BBa_M1603/annotation2173102/range2173102/1
type: Range
persistent identity: range2173102
display id: range2173102
version: 1
top level: BBa_M1603
direction: direction/reverse
start: 2956
end: 3882
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2173095
display id: annotation2173095
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173095/range2173095/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1603/annotation2173095/range2173095/1
type: Range
persistent identity: range2173095
display id: range2173095
version: 1
top level: BBa_M1603
direction: direction/forward
start: 3004
end: 3019
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2173098
display id: annotation2173098
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173098/range2173098/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1603/annotation2173098/range2173098/1
type: Range
persistent identity: range2173098
display id: range2173098
version: 1
top level: BBa_M1603
direction: direction/forward
start: 3755
end: 3834
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2173100
display id: annotation2173100
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173100/range2173100/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1603/annotation2173100/range2173100/1
type: Range
persistent identity: range2173100
display id: range2173100
version: 1
top level: BBa_M1603
direction: direction/forward
start: 3842
end: 3882
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2173103
display id: annotation2173103
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173103/range2173103/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1603/annotation2173103/range2173103/1
type: Range
persistent identity: range2173103
display id: range2173103
version: 1
top level: BBa_M1603
direction: direction/forward
start: 3891
end: 3970
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2173105
display id: annotation2173105
version: 1
top level: BBa_M1603
location: https://synbiohub.org/public/igem/BBa_M1603/annotation2173105/range2173105/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1603/annotation2173105/range2173105/1
type: Range
persistent identity: range2173105
display id: range2173105
version: 1
top level: BBa_M1603
direction: direction/forward
start: 3979
end: 4019
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K136046
type: ComponentDefinition
persistent identity: BBa K136046
display id: BBa_K136046
version: 1
was generated by: iGEM to SBOL conversion
description: envZ*
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:03Z
mutable provenance: bacterial genome of mutated (I281S) strain for EnvZ.
owned by: james
owned by: myers
mutable description: EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3215
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR and ligation in pCR4Blunt-TOPO
star: false
creator: alexandra bouaziz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG
sequence annotation: I281S T->G
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K136046_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation1994304
display id: annotation1994304
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
type: Range
persistent identity: range1994304
display id: range1994304
version: 1
top level: BBa_K136046
direction: direction/forward
start: 1
end: 3
orientation: inline

I281S T->G
type: SequenceAnnotation
persistent identity: annotation1994273
display id: annotation1994273
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
type: Range
persistent identity: range1994273
display id: range1994273
version: 1
top level: BBa_K136046
direction: direction/forward
start: 842
end: 842
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation1994303
display id: annotation1994303
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
type: Range
persistent identity: range1994303
display id: range1994303
version: 1
top level: BBa_K136046
direction: direction/forward
start: 1351
end: 1353
orientation: inline

BBa_M1600
type: ComponentDefinition
persistent identity: BBa M1600
display id: BBa_M1600
version: 1
was generated by: iGEM to SBOL conversion
description: CXCR3
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Aspergillus oryzae
owned by: james
owned by: myers
mutable description: putative type III polyketide synthase (PKS) gene
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12289
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: na
star: false
creator: Yoshitaro Heshiki
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding Sequence
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1600_sequence/1

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2171439
display id: annotation2171439
version: 1
top level: BBa_M1600
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171439/range2171439/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1600/annotation2171439/range2171439/1
type: Range
persistent identity: range2171439
display id: range2171439
version: 1
top level: BBa_M1600
direction: direction/forward
start: 1
end: 1101
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2171438
display id: annotation2171438
version: 1
top level: BBa_M1600
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171438/range2171438/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1600/annotation2171438/range2171438/1
type: Range
persistent identity: range2171438
display id: range2171438
version: 1
top level: BBa_M1600
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2171440
display id: annotation2171440
version: 1
top level: BBa_M1600
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171440/range2171440/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1600/annotation2171440/range2171440/1
type: Range
persistent identity: range2171440
display id: range2171440
version: 1
top level: BBa_M1600
direction: direction/forward
start: 1099
end: 1101
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: pressure inducible lac promoter
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: na
star: false
creator: Masashi Kajita
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K121011
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
top level: BBa_K121011
direction: direction/forward
start: 1
end: 232
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
top level: BBa_K121011
direction: direction/forward
start: 94
end: 131
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
top level: BBa_K121011
direction: direction/forward
start: 141
end: 146
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
top level: BBa_K121011
direction: direction/forward
start: 165
end: 168
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
top level: BBa_K121011
direction: direction/forward
start: 169
end: 203
orientation: inline

BBa_J54103
type: ComponentDefinition
persistent identity: BBa J54103
display id: BBa_J54103
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless-GFP(A)
created: 2006-10-16T11:00:00Z
modified: 2015-08-31T03:48:11Z
mutable provenance: synthesis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: promoterless
discontinued: false
dominant: true
experience: experience/None
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: In stock
status: status/Available
bookmark: true
mutable notes: as negative control
star: true
creator: Yusaku Nakashima
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J54033
sequence annotation: SalI
sequence annotation: BamHI
sequence annotation: BglII
sequence annotation: BBa_J54025
sequence annotation: MulI
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_J54103_sequence/1

BBa_J54033
type: SequenceAnnotation
persistent identity: annotation2152489
display id: annotation2152489
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152489/range2152489/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152489/range2152489/1
type: Range
persistent identity: range2152489
display id: range2152489
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 1
end: 20
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2152490
display id: annotation2152490
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152490/range2152490/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J54103/annotation2152490/range2152490/1
type: Range
persistent identity: range2152490
display id: range2152490
version: 1
top level: BBa_J54103
direction: direction/forward
start: 1
end: 6
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2152491
display id: annotation2152491
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152491/range2152491/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J54103/annotation2152491/range2152491/1
type: Range
persistent identity: range2152491
display id: range2152491
version: 1
top level: BBa_J54103
direction: direction/forward
start: 15
end: 20
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2152493
display id: annotation2152493
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152493/range2152493/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54103/annotation2152493/range2152493/1
type: Range
persistent identity: range2152493
display id: range2152493
version: 1
top level: BBa_J54103
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_J54025
type: SequenceAnnotation
persistent identity: annotation2152492
display id: annotation2152492
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152492/range2152492/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152492/range2152492/1
type: Range
persistent identity: range2152492
display id: range2152492
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 27
end: 46
orientation: inline

MulI
type: SequenceAnnotation
persistent identity: annotation2152494
display id: annotation2152494
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152494/range2152494/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54103/annotation2152494/range2152494/1
type: Range
persistent identity: range2152494
display id: range2152494
version: 1
top level: BBa_J54103
direction: direction/forward
start: 41
end: 46
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2152496
display id: annotation2152496
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152496/range2152496/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152496/range2152496/1
type: Range
persistent identity: range2152496
display id: range2152496
version: 1
top level: BBa_J54103
direction: direction/forward
start: 49
end: 64
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation2152495
display id: annotation2152495
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152495/range2152495/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J54103/annotation2152495/range2152495/1
type: Range
persistent identity: range2152495
display id: range2152495
version: 1
top level: BBa_J54103
direction: direction/forward
start: 49
end: 64
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2152497
display id: annotation2152497
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152497/range2152497/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J54103/annotation2152497/range2152497/1
type: Range
persistent identity: range2152497
display id: range2152497
version: 1
top level: BBa_J54103
direction: direction/forward
start: 56
end: 62
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152498
display id: annotation2152498
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152498/range2152498/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152498/range2152498/1
type: Range
persistent identity: range2152498
display id: range2152498
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 71
end: 790
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152499
display id: annotation2152499
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152499/range2152499/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152499/range2152499/1
type: Range
persistent identity: range2152499
display id: range2152499
version: 1
top level: BBa_J54103
direction: direction/forward
start: 800
end: 879
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152501
display id: annotation2152501
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152501/range2152501/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J54103/annotation2152501/range2152501/1
type: Range
persistent identity: range2152501
display id: range2152501
version: 1
top level: BBa_J54103
direction: direction/forward
start: 811
end: 854
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152500
display id: annotation2152500
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152500/range2152500/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152500/range2152500/1
type: Range
persistent identity: range2152500
display id: range2152500
version: 1
top level: BBa_J54103
direction: direction/forward
start: 887
end: 927
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152502
display id: annotation2152502
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152502/range2152502/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J54103/annotation2152502/range2152502/1
type: Range
persistent identity: range2152502
display id: range2152502
version: 1
top level: BBa_J54103
direction: direction/forward
start: 899
end: 918
orientation: inline

https://synbiohub.org/public/igem/BBa_M1603_sequence/1
type: Sequence
persistent identity: BBa M1603 sequence
display id: BBa_M1603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagattaaagaggagaaatactagatggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K136046_sequence/1
type: Sequence
persistent identity: BBa K136046 sequence
display id: BBa_K136046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1600_sequence/1
type: Sequence
persistent identity: BBa M1600 sequence
display id: BBa_M1600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaatataattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatggcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttgtcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctgggcgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcattgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactggggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggcatcgtattcaggcttatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K121011_sequence/1
type: Sequence
persistent identity: BBa K121011 sequence
display id: BBa_K121011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J54103_sequence/1
type: Sequence
persistent identity: BBa J54103 sequence
display id: BBa_J54103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z