BBa_M1607
1
BBa_M1607
Protein used to add two hydroxyl groups to pyrene
2012-03-27T11:00:00Z
2015-05-08T01:13:57Z
Gene sequence retrieved from Mycobacterium sp. KMS.
This uses basic part BBa_M1107 to make a composite part for the dioxygenation of pyrene. This is one of the initial enzymes used in a known pathway to degrade high molecular weight polycyclic aromatic hydrocarbons
false
false
_768_
0
12579
9
Not in stock
false
The amino acid sequence was used to back-translate to an E. coli compatible codon table usage.
false
Keith Albretsen
component2171550
1
BBa_M1107
component2171557
1
BBa_B0015
component2171548
1
BBa_B0030
component2171546
1
BBa_J45992
annotation2171550
1
BBa_M1107
range2171550
1
229
738
annotation2171546
1
BBa_J45992
range2171546
1
1
199
annotation2171548
1
BBa_B0030
range2171548
1
208
222
annotation2171557
1
BBa_B0015
range2171557
1
747
875
BBa_J45992
1
p(osmY)
Full-length stationary phase osmY promoter
2006-07-10T11:00:00Z
2015-08-31T04:08:50Z
This part was PCRed out of E. coli's genome.
osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
false
false
_84_
0
642
84
Not in stock
true
Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
Total Length: 49 bp
Annealing Length: 21 bp
GC Content: 38.1%
Melting Temperature: 49.5 degrees C
hairpin deltaG: -2.66 kcal/mol
self dimer deltaG: -99.97 kcal/mol
Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
Total Length: 47 bp
Annealing Length: 19 bp
GC Content: 52.6%
Melting Temperature: 53.4 degrees C
hairpin deltaG: -3.99 kcal/mol
self dimer deltaG: -98.3 kcal/mol
Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
Total Length: 54 bp
Annealing Length: 25 bp
GC Content: 20.0%
Melting Temperature: 46.4 degrees C
hairpin deltaG: -2.86 kcal/mol
self dimer deltaG: -99.44 kcal/mol
heterodimer deltaG with Forward Primer: -99.44 kcal/mol
heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
false
Stephen Payne
annotation1883646
1
-35
range1883646
1
164
169
annotation1883645
1
-10
range1883645
1
188
193
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_M1107
1
BBa_M1107
aromatic ring hydroxylating dioxygenase
2012-03-27T11:00:00Z
2015-05-08T01:13:52Z
Comes from the genome(s) of M. sp. KMS (as well as M. sp. JLS and M. sp. MCS).
Beta subunit of aromatic ring hydroxylating dioxygenase found in Mycobacterium sp. KMS. This protein is used in the pathway identified to degrade pyrene and other polycyclic aromatic hydrocarbons.
false
true
_768_
0
12579
9
Not in stock
false
The amino acid sequence was used to back-translate into a DNA sequence more compatible with E. coli codon encoding through DNA 2.0.
false
Keith Albretsen
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_M1607_sequence
1
ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagatgaacgcggtggctgttgaccaggatactcgcgaagcggttgaggagttcatgttccgtgaagctgaactgctcgacggaggacaattccgcgagtggctgggcttgttagaccctgatatcagatatgtcgtgcctgtgcggacgactcgcgaagactccgcaggatgggtgggtgcaatcgcacactggaatgatgattacaccggcctggaaatgcgggttctgcgcggcgagaccgatttttcgtgggctgaaagcccacgttctcgtactcgtcattttgttagtaatatccggactacagccggtccagaagctgatgaattaacggtgcgtagcaatcttctcttcttcaggtcgcgcggcgattcgggccgctgggaactgttatcagccgaacgtgtggacgttcttcgccgcacggatgatagcctgcggcttgccagaagagaagttctcctagaccattctacccttcctattgacaatttatcagtgtttttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M1107_sequence
1
atgaacgcggtggctgttgaccaggatactcgcgaagcggttgaggagttcatgttccgtgaagctgaactgctcgacggaggacaattccgcgagtggctgggcttgttagaccctgatatcagatatgtcgtgcctgtgcggacgactcgcgaagactccgcaggatgggtgggtgcaatcgcacactggaatgatgattacaccggcctggaaatgcgggttctgcgcggcgagaccgatttttcgtgggctgaaagcccacgttctcgtactcgtcattttgttagtaatatccggactacagccggtccagaagctgatgaattaacggtgcgtagcaatcttctcttcttcaggtcgcgcggcgattcgggccgctgggaactgttatcagccgaacgtgtggacgttcttcgccgcacggatgatagcctgcggcttgccagaagagaagttctcctagaccattctacccttcctattgacaatttatcagtgtttttatag
BBa_J45992_sequence
1
ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z