BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J45992 1 p(osmY) Full-length stationary phase osmY promoter 2006-07-10T11:00:00Z 2015-08-31T04:08:50Z This part was PCRed out of E. coli's genome. osmY's promoter is active in stationary phase and under high osmotic pressure conditions. false false _84_ 0 642 84 Not in stock true Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol false Stephen Payne annotation1883646 1 -35 range1883646 1 164 169 annotation1883645 1 -10 range1883645 1 188 193 BBa_M1107 1 BBa_M1107 aromatic ring hydroxylating dioxygenase 2012-03-27T11:00:00Z 2015-05-08T01:13:52Z Comes from the genome(s) of M. sp. KMS (as well as M. sp. JLS and M. sp. MCS). Beta subunit of aromatic ring hydroxylating dioxygenase found in Mycobacterium sp. KMS. This protein is used in the pathway identified to degrade pyrene and other polycyclic aromatic hydrocarbons. false true _768_ 0 12579 9 Not in stock false The amino acid sequence was used to back-translate into a DNA sequence more compatible with E. coli codon encoding through DNA 2.0. false Keith Albretsen BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_M1607 1 BBa_M1607 Protein used to add two hydroxyl groups to pyrene 2012-03-27T11:00:00Z 2015-05-08T01:13:57Z Gene sequence retrieved from Mycobacterium sp. KMS. This uses basic part BBa_M1107 to make a composite part for the dioxygenation of pyrene. This is one of the initial enzymes used in a known pathway to degrade high molecular weight polycyclic aromatic hydrocarbons false false _768_ 0 12579 9 Not in stock false The amino acid sequence was used to back-translate to an E. coli compatible codon table usage. false Keith Albretsen component2171546 1 BBa_J45992 component2171548 1 BBa_B0030 component2171550 1 BBa_M1107 component2171557 1 BBa_B0015 annotation2171548 1 BBa_B0030 range2171548 1 208 222 annotation2171557 1 BBa_B0015 range2171557 1 747 875 annotation2171550 1 BBa_M1107 range2171550 1 229 738 annotation2171546 1 BBa_J45992 range2171546 1 1 199 BBa_M1607_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagatgaacgcggtggctgttgaccaggatactcgcgaagcggttgaggagttcatgttccgtgaagctgaactgctcgacggaggacaattccgcgagtggctgggcttgttagaccctgatatcagatatgtcgtgcctgtgcggacgactcgcgaagactccgcaggatgggtgggtgcaatcgcacactggaatgatgattacaccggcctggaaatgcgggttctgcgcggcgagaccgatttttcgtgggctgaaagcccacgttctcgtactcgtcattttgttagtaatatccggactacagccggtccagaagctgatgaattaacggtgcgtagcaatcttctcttcttcaggtcgcgcggcgattcgggccgctgggaactgttatcagccgaacgtgtggacgttcttcgccgcacggatgatagcctgcggcttgccagaagagaagttctcctagaccattctacccttcctattgacaatttatcagtgtttttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M1107_sequence 1 atgaacgcggtggctgttgaccaggatactcgcgaagcggttgaggagttcatgttccgtgaagctgaactgctcgacggaggacaattccgcgagtggctgggcttgttagaccctgatatcagatatgtcgtgcctgtgcggacgactcgcgaagactccgcaggatgggtgggtgcaatcgcacactggaatgatgattacaccggcctggaaatgcgggttctgcgcggcgagaccgatttttcgtgggctgaaagcccacgttctcgtactcgtcattttgttagtaatatccggactacagccggtccagaagctgatgaattaacggtgcgtagcaatcttctcttcttcaggtcgcgcggcgattcgggccgctgggaactgttatcagccgaacgtgtggacgttcttcgccgcacggatgatagcctgcggcttgccagaagagaagttctcctagaccattctacccttcctattgacaatttatcagtgtttttatag BBa_J45992_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa BBa_B0030_sequence 1 attaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z