LOCUS       BBa_M1612               1231 bp    DNA     linear       27-Jun-2024
DEFINITION  mioC promoter + RBS + tetR (standard 10 compatible)
ACCESSION   BBa_M1612
VERSION     BBa_M1612.1  
FEATURES             Location/Qualifiers
     misc_feature    1..444
                     /label=BBa_M1610
     promoter        1..444
                     /label=BBa_M1610
     RBS             453..467
                     /label=paskiba3_rbs
     CDS             468..1094
                     /label=paskiba3_TetR
     misc_feature    453..1094
                     /label=BBa_I15040
     stem_loop       1114..1157
                     /label=stem_loop
     misc_feature    1103..1182
                     /label=BBa_B0010
     terminator      1103..1182
                     /label=BBa_B0010
     stem_loop       1198..1217
                     /label=T7 TE
     misc_feature    1224..1224
                     /label=stop
     polyA_site      1218..1231
                     /label=polya
     misc_feature    1191..1231
                     /label=BBa_B0012
     terminator      1191..1231
                     /label=BBa_B0012
     terminator      1103..1231
                     /label=BBa_B0015
ORIGIN
        1 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
       61 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
      121 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc
      181 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
      241 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
      301 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
      361 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
      421 gctgataaga gtgatatctg ccattactag agcattggta ggaattaatg atgtctcgtt
      481 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      541 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      601 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      661 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      721 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      781 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      841 gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt
      901 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      961 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1021 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1081 aaagtgggtc ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1201 tcaccttcgg gtgggccttt ctgcgtttat a
//