LOCUS       BBa_M1613               1330 bp    DNA     linear       22-Nov-2024
DEFINITION  mioC promoter + RBS + GFP + double terminator (standard 10 
            compatible)
ACCESSION   BBa_M1613
VERSION     BBa_M1613.1  
FEATURES             Location/Qualifiers
     misc_feature    1..444
                     /label=BBa_M1610
     promoter        1..444
                     /label=BBa_M1610
     RBS             453..467
                     /label=RBS-1\Strong
     RBS             460..464
                     /label=RBS
     misc_feature    453..467
                     /label=BBa_B0030
     RBS             453..467
                     /label=BBa_B0030
     CDS             474..1193
                     /label=GFP protein
     CDS             474..1193
                     /label=BBa_E0040
     stem_loop       1213..1256
                     /label=stem_loop
     misc_feature    1202..1281
                     /label=BBa_B0010
     terminator      1202..1281
                     /label=BBa_B0010
     stem_loop       1297..1316
                     /label=T7 TE
     misc_feature    1323..1323
                     /label=stop
     polyA_site      1317..1330
                     /label=polya
     misc_feature    1290..1330
                     /label=BBa_B0012
     terminator      1290..1330
                     /label=BBa_B0012
     terminator      1202..1330
                     /label=BBa_B0015
ORIGIN
        1 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
       61 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
      121 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc
      181 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
      241 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
      301 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
      361 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
      421 gctgataaga gtgatatctg ccattactag agattaaaga ggagaaatac tagatgcgta
      481 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      541 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      601 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      661 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      721 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      781 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      841 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      901 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      961 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1021 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1081 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1141 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1201 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1261 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1321 tgcgtttata
//