LOCUS       BBa_M1614               2277 bp    DNA     linear       22-Nov-2024
DEFINITION  Pr promoter+RBS+ XylR + terminator
ACCESSION   BBa_M1614
VERSION     BBa_M1614.1  
FEATURES             Location/Qualifiers
     promoter        1..410
                     /label=Pr
     promoter        1..410
                     /label=BBa_I723018
     RBS             419..433
                     /label=RBS-1\Strong
     RBS             426..430
                     /label=RBS
     misc_feature    419..433
                     /label=BBa_B0030
     RBS             419..433
                     /label=BBa_B0030
     CDS             440..2140
                     /label=BBa_M1611
     stem_loop       2160..2203
                     /label=stem_loop
     misc_feature    2149..2228
                     /label=BBa_B0010
     terminator      2149..2228
                     /label=BBa_B0010
     stem_loop       2244..2263
                     /label=T7 TE
     misc_feature    2270..2270
                     /label=stop
     polyA_site      2264..2277
                     /label=polya
     misc_feature    2237..2277
                     /label=BBa_B0012
     terminator      2237..2277
                     /label=BBa_B0012
     terminator      2149..2277
                     /label=BBa_B0015
ORIGIN
        1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct
       61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca
      121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag
      181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg
      241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca
      301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt
      361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagat
      421 taaagaggag aaatactaga tgtcgcttac atacaaaccc aagatgcagc atgaggatat
      481 gcaagacctt agcagccaga tccgtttcgt tgccgccgaa ggcaagatct ggttgggaga
      541 gcagcgcatg ctcgtaatgc agctatctac gctggccagc ttccgtcgcg aaattatcag
      601 cttgatcggc gtcgagcggg ccaagggttt cttcctgcgg ttgggctatc agtccggcct
      661 gatggatgcc gagctggcac gcaagctgcg gccggccatg cgcgaggagg aggtgttcct
      721 ggctgggcct caattgtatg cgctcaaggg gatggtcaaa gtacgcttgc tgacaatgga
      781 tatcgccatc cgggacggac gtttcaacgt ggaggccgag tggattgatt cctttgaagt
      841 ggatatctgc cgaactgagc tgggcctgat gaatgagccc gtctgctgga cggtgctagg
      901 ctatgctagc ggctatggtt cggcattcat gggccgcaga atcattttcc aggaaactag
      961 ctgtcgcggg tgcggtgacg ataaatgcct tatcgtcggc aagaccgcag aagagtgggg
     1021 cgatgtcagc agtttcgaag cctacttcaa aagcgacccg atcgtagacg agcgctacga
     1081 gctacagacc caggttgcca acctgcgcaa ccgcctgaag cagtacgatg ggcagtatta
     1141 cggcattggc cattcgccag cctacaagcg catctgtgag accatcgaca aggctgcacg
     1201 cggcagggtt tcggtcctgc tactgggtga gactggggtg ggcaaggagg taatcgcgcg
     1261 cagcgtgcat ttgcgcagtg agcgcgcaga gcaacccttc gtcgcggtga actgtgcggc
     1321 aattccgccg gatctgatcg agtcggaact gtttggtgtc gataagggcg cctatacggg
     1381 cgcggtcaat gcacgcgctg gacgttttga acgggccaac ggcggcacca tctttcttga
     1441 tgaggtgatc gaattgacgc cgagggccca ggccaccctg ctacgggtat tgcaggaagg
     1501 agagctagag cgggtcggcg gcgaccgcac gcgaaaggtc gacgtgaggt taatcaccgc
     1561 aacaaacgag aacctggaag aggcggtcaa gatggggcgc tttcgcgcag acctgttctt
     1621 tcggctgaat gtttttcccg tgcatatccc gccgttgcgc gagcgcgtgg aagatatccc
     1681 gctgctggtc gagcattttc ttagaaggca ccataaggaa tacggtaaga agactcttgg
     1741 cctgtctgat cgagcgatgg aggcctgcct ccactaccaa tggccaggca atatccgcga
     1801 gctggagaac gcccttgagc gcggggtgat tcttaccgag agcaacgaaa gcatcaatgt
     1861 cgagtcgctg ttcccggggt tggcgacggc taccgaaggc gacaggctat cgagcgaggg
     1921 ccggttggag gaggagtccg gtgacagttg gtttaggcaa attatcgacc agggcgtcag
     1981 cctcgaagat ctcgaagcgg gtttaatgcg cacggccatg gaccgttgtg ggcagaatat
     2041 ctcacaggcg gcgcggttgc tgggattgac ccgcccggca atggcctatc gacttaagaa
     2101 gcttgacccc agcttatctg tgaaagcaat gggccgatag tactagagcc aggcatcaaa
     2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//