BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_M1614
1
BBa_M1614
Pr promoter+RBS+ XylR + terminator
2013-04-11T11:00:00Z
2015-05-08T01:13:57Z
Pr: BBa_I723018
xylR: BBa_M1611
Pu:BBa_I723020
eeeee
false
false
_768_
0
16142
9
Not in stock
false
Standar 10 compatible
false
Kandy Napan
component2215989
1
BBa_M1611
component2215996
1
BBa_B0015
component2215985
1
BBa_I723018
component2215987
1
BBa_B0030
annotation2215987
1
BBa_B0030
range2215987
1
419
433
annotation2215985
1
BBa_I723018
range2215985
1
1
410
annotation2215996
1
BBa_B0015
range2215996
1
2149
2277
annotation2215989
1
BBa_M1611
range2215989
1
440
2140
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_I723018
1
Pr
Pr (promoter for XylR)
2007-10-24T11:00:00Z
2015-08-31T04:07:54Z
[Christine/Maia]
This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
false
false
_126_
0
2140
9
Not in stock
false
[Christine/Maia]
false
Scott Ramsay
annotation1955191
1
Pr
range1955191
1
1
410
BBa_M1611
1
BBa_M1611
XylR
2013-04-11T11:00:00Z
2015-05-08T01:13:57Z
From a complete genomic sequence of Escherichia coli p0305293.5 gecp03052935.contig.229
The MioC Promoter is activated by the presence of DNAa which is produced in the S-Phase of bacterial replication. Isolated from Escherichia coli p0305293.5 gecp03052935.contig.229
false
false
_768_
0
16146
9
Not in stock
false
aaaattttcccc
false
Nathan Israelsen
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_M1614_sequence
1
attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagattaaagaggagaaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M1611_sequence
1
atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I723018_sequence
1
attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z