LOCUS       BBa_M1615               2179 bp    DNA     linear       27-Jun-2024
DEFINITION  MioCpromoter+RBS tetR+stop+tetp+RBS+GFP+stop
ACCESSION   BBa_M1615
VERSION     BBa_M1615.1  
FEATURES             Location/Qualifiers
     misc_feature    1..444
                     /label=BBa_M1610
     promoter        1..444
                     /label=BBa_M1610
     RBS             453..467
                     /label=paskiba3_rbs
     CDS             468..1094
                     /label=paskiba3_TetR
     misc_feature    453..1094
                     /label=BBa_I15040
     stem_loop       1114..1157
                     /label=stem_loop
     misc_feature    1103..1182
                     /label=BBa_B0010
     terminator      1103..1182
                     /label=BBa_B0010
     stem_loop       1198..1217
                     /label=T7 TE
     misc_feature    1224..1224
                     /label=stop
     polyA_site      1218..1231
                     /label=polya
     misc_feature    1191..1231
                     /label=BBa_B0012
     terminator      1191..1231
                     /label=BBa_B0012
     terminator      1103..1231
                     /label=BBa_B0015
     misc_feature    1..1231
                     /label=BBa_M1612
     misc_feature    1240..1258
                     /label=TetR 1
     misc_feature    1240..1293
                     /label=BBa_R0040
     promoter        1259..1264
                     /label=-35
     misc_feature    1265..1283
                     /label=TetR 2
     promoter        1282..1287
                     /label=-10
     promoter        1240..1293
                     /label=BBa_R0040
     RBS             1302..1316
                     /label=RBS-1\Strong
     RBS             1309..1313
                     /label=RBS
     misc_feature    1302..1316
                     /label=BBa_B0030
     RBS             1302..1316
                     /label=BBa_B0030
     CDS             1323..2042
                     /label=GFP protein
     CDS             1323..2042
                     /label=BBa_E0040
     stem_loop       2062..2105
                     /label=stem_loop
     misc_feature    2051..2130
                     /label=BBa_B0010
     terminator      2051..2130
                     /label=BBa_B0010
     stem_loop       2146..2165
                     /label=T7 TE
     misc_feature    2172..2172
                     /label=stop
     polyA_site      2166..2179
                     /label=polya
     misc_feature    2139..2179
                     /label=BBa_B0012
     terminator      2139..2179
                     /label=BBa_B0012
     terminator      2051..2179
                     /label=BBa_B0015
ORIGIN
        1 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
       61 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
      121 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc
      181 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
      241 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
      301 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
      361 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
      421 gctgataaga gtgatatctg ccattactag agcattggta ggaattaatg atgtctcgtt
      481 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      541 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      601 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      661 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      721 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      781 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      841 gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt
      901 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      961 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1021 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1081 aaagtgggtc ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1201 tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt
     1261 gacatcccta tcagtgatag agatactgag cactactaga gattaaagag gagaaatact
     1321 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1381 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1441 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1501 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1561 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1621 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1681 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1741 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1801 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1861 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1921 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1981 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     2041 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2101 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2161 gggcctttct gcgtttata
//