BBa_M1615
type: ComponentDefinition
persistent identity: BBa M1615
display id: BBa_M1615
version: 1
was generated by: iGEM to SBOL conversion
description: MioCpromoter+RBS tetR+stop+tetp+RBS+GFP+stop
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: BBa_M1612: MioCpromoter+RBS tetR+stop BBa_R0040: tetp BBa_B0030: RBS BBa_E0040:GFP BBa_B0015: double terminator
owned by: james
owned by: myers
mutable description: In the S phase of the cell cycle the MioC promoter is inactivated by DnaA protein, as a result the tetR is not transcribed and the tetP is not negatively controlled by the tetR. So, GFP reporter is synthesized.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16142
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standard 10 compatible, with scars.
star: false
creator: Kandy Napan
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_B0015
component: BBa_E0040
component: BBa_B0030
component: BBa_R0040
component: BBa_M1612
sequence annotation: BBa_M1612
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1615_sequence/1

BBa_B0015
type: Component
persistent identity: component2215755
display id: component2215755
version: 1
top level: BBa_M1615
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2215748
display id: component2215748
version: 1
top level: BBa_M1615
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2215745
display id: component2215745
version: 1
top level: BBa_M1615
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component2215739
display id: component2215739
version: 1
top level: BBa_M1615
definition: p(tetR)
access: public

BBa_M1612
type: Component
persistent identity: component2215738
display id: component2215738
version: 1
top level: BBa_M1615
definition: BBa_M1612
access: public

BBa_M1612
type: SequenceAnnotation
persistent identity: annotation2215738
display id: annotation2215738
version: 1
top level: BBa_M1615
location: https://synbiohub.org/public/igem/BBa_M1615/annotation2215738/range2215738/1
component: BBa_M1612

https://synbiohub.org/public/igem/BBa_M1615/annotation2215738/range2215738/1
type: Range
persistent identity: range2215738
display id: range2215738
version: 1
top level: BBa_M1615
direction: direction/reverse
start: 1
end: 1231
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2215739
display id: annotation2215739
version: 1
top level: BBa_M1615
location: https://synbiohub.org/public/igem/BBa_M1615/annotation2215739/range2215739/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1615/annotation2215739/range2215739/1
type: Range
persistent identity: range2215739
display id: range2215739
version: 1
top level: BBa_M1615
direction: direction/forward
start: 1240
end: 1293
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2215745
display id: annotation2215745
version: 1
top level: BBa_M1615
location: https://synbiohub.org/public/igem/BBa_M1615/annotation2215745/range2215745/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1615/annotation2215745/range2215745/1
type: Range
persistent identity: range2215745
display id: range2215745
version: 1
top level: BBa_M1615
direction: direction/forward
start: 1302
end: 1316
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2215748
display id: annotation2215748
version: 1
top level: BBa_M1615
location: https://synbiohub.org/public/igem/BBa_M1615/annotation2215748/range2215748/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_M1615/annotation2215748/range2215748/1
type: Range
persistent identity: range2215748
display id: range2215748
version: 1
top level: BBa_M1615
direction: direction/reverse
start: 1323
end: 2042
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2215755
display id: annotation2215755
version: 1
top level: BBa_M1615
location: https://synbiohub.org/public/igem/BBa_M1615/annotation2215755/range2215755/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1615/annotation2215755/range2215755/1
type: Range
persistent identity: range2215755
display id: range2215755
version: 1
top level: BBa_M1615
direction: direction/reverse
start: 2051
end: 2179
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_M1612
type: ComponentDefinition
persistent identity: BBa M1612
display id: BBa_M1612
version: 1
was generated by: iGEM to SBOL conversion
description: mioC promoter + RBS + tetR (standard 10 compatible)
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: mioC: Escherichia coli p0305293.5 gecp03052935.contig.229, whole genome shotgun sequence. RBS + tetR: BBa_I15040
owned by: james
owned by: myers
mutable description: The MioC promoter is a cell-cycle dependent promoter. Nathan knows this!!! he will modified
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16130
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard 10 compatible
star: false
creator: Maureen Kesaano
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_I15040
component: BBa_M1610
sequence annotation: BBa_M1610
sequence annotation: BBa_I15040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1612_sequence/1

BBa_B0015
type: Component
persistent identity: component2215714
display id: component2215714
version: 1
top level: BBa_M1612
definition: BBa_B0015
access: public

BBa_I15040
type: Component
persistent identity: component2215707
display id: component2215707
version: 1
top level: BBa_M1612
definition: RBS.TetR
access: public

BBa_M1610
type: Component
persistent identity: component2215703
display id: component2215703
version: 1
top level: BBa_M1612
definition: BBa_M1610
access: public

BBa_M1610
type: SequenceAnnotation
persistent identity: annotation2215703
display id: annotation2215703
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215703/range2215703/1
component: BBa_M1610

https://synbiohub.org/public/igem/BBa_M1612/annotation2215703/range2215703/1
type: Range
persistent identity: range2215703
display id: range2215703
version: 1
top level: BBa_M1612
direction: direction/forward
start: 1
end: 444
orientation: inline

BBa_I15040
type: SequenceAnnotation
persistent identity: annotation2215707
display id: annotation2215707
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215707/range2215707/1
component: BBa_I15040

https://synbiohub.org/public/igem/BBa_M1612/annotation2215707/range2215707/1
type: Range
persistent identity: range2215707
display id: range2215707
version: 1
top level: BBa_M1612
direction: direction/reverse
start: 453
end: 1094
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2215714
display id: annotation2215714
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215714/range2215714/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1612/annotation2215714/range2215714/1
type: Range
persistent identity: range2215714
display id: range2215714
version: 1
top level: BBa_M1612
direction: direction/reverse
start: 1103
end: 1231
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_M1610
type: ComponentDefinition
persistent identity: BBa M1610
display id: BBa_M1610
version: 1
was generated by: iGEM to SBOL conversion
description: MioC Promoter
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: From a complete genomic sequence of Escherichia coli p0305293.5 gecp03052935.contig.229
owned by: james
owned by: myers
mutable description: The MioC Promoter is activated by the presence of DNAa which is produced in the S-Phase of bacterial replication. Isolated from Escherichia coli p0305293.5 gecp03052935.contig.229
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16146
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standard 10 compatible
star: false
creator: Nathan Israelsen
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_M1610
sequence: https://synbiohub.org/public/igem/BBa_M1610_sequence/1

BBa_M1610
type: SequenceAnnotation
persistent identity: annotation2214866
display id: annotation2214866
version: 1
top level: BBa_M1610
location: https://synbiohub.org/public/igem/BBa_M1610/annotation2214866/range2214866/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1610/annotation2214866/range2214866/1
type: Range
persistent identity: range2214866
display id: range2214866
version: 1
top level: BBa_M1610
direction: direction/forward
start: 1
end: 444
orientation: inline

RBS.TetR
type: ComponentDefinition
persistent identity: BBa I15040
display id: BBa_I15040
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.TetR from pASKIBA3
created: 2005-06-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: pASKIBA3 (IBA biotagnology)
owned by: james
owned by: myers
mutable description: The part accepts POPS to produce TetR. This sequence encodes the ribosome binding site and tetracycline repressor protein from the plasmid pASKIBA3.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: paskiba3_rbs
sequence annotation: paskiba3_TetR
sequence: https://synbiohub.org/public/igem/BBa_I15040_sequence/1

paskiba3_rbs
type: SequenceAnnotation
persistent identity: annotation1501145
display id: annotation1501145
version: 1
top level: RBS.TetR
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
type: Range
persistent identity: range1501145
display id: range1501145
version: 1
top level: RBS.TetR
direction: direction/forward
start: 1
end: 15
orientation: inline

paskiba3_TetR
type: SequenceAnnotation
persistent identity: annotation1501146
display id: annotation1501146
version: 1
top level: RBS.TetR
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
type: Range
persistent identity: range1501146
display id: range1501146
version: 1
top level: RBS.TetR
direction: direction/forward
start: 16
end: 642
orientation: inline

https://synbiohub.org/public/igem/BBa_M1615_sequence/1
type: Sequence
persistent identity: BBa M1615 sequence
display id: BBa_M1615_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccattactagagcattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1612_sequence/1
type: Sequence
persistent identity: BBa M1612 sequence
display id: BBa_M1612_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccattactagagcattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1610_sequence/1
type: Sequence
persistent identity: BBa M1610 sequence
display id: BBa_M1610_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15040_sequence/1
type: Sequence
persistent identity: BBa I15040 sequence
display id: BBa_I15040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z