LOCUS BBa_M1616 3491 bp DNA linear 25-Nov-2024 DEFINITION Pr+ XylR + Pu + GFP ACCESSION BBa_M1616 VERSION BBa_M1616.1 FEATURES Location/Qualifiers promoter 1..410 /label=Pr promoter 1..410 /label=BBa_I723018 RBS 419..433 /label=RBS-1\Strong RBS 426..430 /label=RBS misc_feature 419..433 /label=BBa_B0030 RBS 419..433 /label=BBa_B0030 CDS 440..2140 /label=BBa_M1611 stem_loop 2160..2203 /label=stem_loop misc_feature 2149..2228 /label=BBa_B0010 terminator 2149..2228 /label=BBa_B0010 stem_loop 2244..2263 /label=T7 TE misc_feature 2270..2270 /label=stop polyA_site 2264..2277 /label=polya misc_feature 2237..2277 /label=BBa_B0012 terminator 2237..2277 /label=BBa_B0012 terminator 2149..2277 /label=BBa_B0015 misc_feature 1..2277 /label=BBa_M1614 promoter 2286..2605 /label=Pu promoter 2286..2605 /label=BBa_I723020 RBS 2614..2628 /label=RBS-1\Strong RBS 2621..2625 /label=RBS misc_feature 2614..2628 /label=BBa_B0030 RBS 2614..2628 /label=BBa_B0030 CDS 2635..3354 /label=GFP protein CDS 2635..3354 /label=BBa_E0040 stem_loop 3374..3417 /label=stem_loop misc_feature 3363..3442 /label=BBa_B0010 terminator 3363..3442 /label=BBa_B0010 stem_loop 3458..3477 /label=T7 TE misc_feature 3484..3484 /label=stop polyA_site 3478..3491 /label=polya misc_feature 3451..3491 /label=BBa_B0012 terminator 3451..3491 /label=BBa_B0012 terminator 3363..3491 /label=BBa_B0015 ORIGIN 1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct 61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca 121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag 181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg 241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca 301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt 361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagat 421 taaagaggag aaatactaga tgtcgcttac atacaaaccc aagatgcagc atgaggatat 481 gcaagacctt agcagccaga tccgtttcgt tgccgccgaa ggcaagatct ggttgggaga 541 gcagcgcatg ctcgtaatgc agctatctac gctggccagc ttccgtcgcg aaattatcag 601 cttgatcggc gtcgagcggg ccaagggttt cttcctgcgg ttgggctatc agtccggcct 661 gatggatgcc gagctggcac gcaagctgcg gccggccatg cgcgaggagg aggtgttcct 721 ggctgggcct caattgtatg cgctcaaggg gatggtcaaa gtacgcttgc tgacaatgga 781 tatcgccatc cgggacggac gtttcaacgt ggaggccgag tggattgatt cctttgaagt 841 ggatatctgc cgaactgagc tgggcctgat gaatgagccc gtctgctgga cggtgctagg 901 ctatgctagc ggctatggtt cggcattcat gggccgcaga atcattttcc aggaaactag 961 ctgtcgcggg tgcggtgacg ataaatgcct tatcgtcggc aagaccgcag aagagtgggg 1021 cgatgtcagc agtttcgaag cctacttcaa aagcgacccg atcgtagacg agcgctacga 1081 gctacagacc caggttgcca acctgcgcaa ccgcctgaag cagtacgatg ggcagtatta 1141 cggcattggc cattcgccag cctacaagcg catctgtgag accatcgaca aggctgcacg 1201 cggcagggtt tcggtcctgc tactgggtga gactggggtg ggcaaggagg taatcgcgcg 1261 cagcgtgcat ttgcgcagtg agcgcgcaga gcaacccttc gtcgcggtga actgtgcggc 1321 aattccgccg gatctgatcg agtcggaact gtttggtgtc gataagggcg cctatacggg 1381 cgcggtcaat gcacgcgctg gacgttttga acgggccaac ggcggcacca tctttcttga 1441 tgaggtgatc gaattgacgc cgagggccca ggccaccctg ctacgggtat tgcaggaagg 1501 agagctagag cgggtcggcg gcgaccgcac gcgaaaggtc gacgtgaggt taatcaccgc 1561 aacaaacgag aacctggaag aggcggtcaa gatggggcgc tttcgcgcag acctgttctt 1621 tcggctgaat gtttttcccg tgcatatccc gccgttgcgc gagcgcgtgg aagatatccc 1681 gctgctggtc gagcattttc ttagaaggca ccataaggaa tacggtaaga agactcttgg 1741 cctgtctgat cgagcgatgg aggcctgcct ccactaccaa tggccaggca atatccgcga 1801 gctggagaac gcccttgagc gcggggtgat tcttaccgag agcaacgaaa gcatcaatgt 1861 cgagtcgctg ttcccggggt tggcgacggc taccgaaggc gacaggctat cgagcgaggg 1921 ccggttggag gaggagtccg gtgacagttg gtttaggcaa attatcgacc agggcgtcag 1981 cctcgaagat ctcgaagcgg gtttaatgcg cacggccatg gaccgttgtg ggcagaatat 2041 ctcacaggcg gcgcggttgc tgggattgac ccgcccggca atggcctatc gacttaagaa 2101 gcttgacccc agcttatctg tgaaagcaat gggccgatag tactagagcc aggcatcaaa 2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2281 tagagcccgg gaaagcgcga tgaacctttt ttatcgctgc cttgatcaaa tcgacaggtg 2341 gttatgcgcg attgatgatt tgctcaaata cagccagcgt gctgtagatt ttctctcata 2401 cccccccttt cttttttaca aagaaaatca ataatttaga tgaaataagg ggatcggtat 2461 aagcaatggc atggcggttg ctagctatac gagacttaaa ataaaaatag tggtgaccct 2521 tcaatgttgt attttctcaa ctctgttcag attggttgct ttcgccatgt atatcctcaa 2581 agcgggccag ccgtagccgt tacgctacta gagattaaag aggagaaata ctagatgcgt 2641 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 2701 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 2761 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 2821 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 2881 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 2941 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 3001 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 3061 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 3121 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 3181 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 3241 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 3301 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 3361 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3421 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3481 ctgcgtttat a //