BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M1614 1 BBa_M1614 Pr promoter+RBS+ XylR + terminator 2013-04-11T11:00:00Z 2015-05-08T01:13:57Z Pr: BBa_I723018 xylR: BBa_M1611 Pu:BBa_I723020 eeeee false false _768_ 0 16142 9 Not in stock false Standar 10 compatible false Kandy Napan component2215985 1 BBa_I723018 component2215996 1 BBa_B0015 component2215987 1 BBa_B0030 component2215989 1 BBa_M1611 annotation2215985 1 BBa_I723018 range2215985 1 1 410 annotation2215989 1 BBa_M1611 range2215989 1 440 2140 annotation2215996 1 BBa_B0015 range2215996 1 2149 2277 annotation2215987 1 BBa_B0030 range2215987 1 419 433 BBa_M1616 1 BBa_M1616 Pr+ XylR + Pu + GFP 2013-04-14T11:00:00Z 2015-05-08T01:13:57Z BBa_M1614: Pr+ XylR + Pu BBa_E0040: GFP dsdfsdsd false false _768_ 0 17020 9 Not in stock false standard 10 compatible false kun zhang component2216582 1 BBa_E0040 component2216575 1 BBa_M1614 component2216589 1 BBa_B0015 component2216579 1 BBa_B0030 component2216577 1 BBa_I723020 annotation2216575 1 BBa_M1614 range2216575 1 1 2277 annotation2216577 1 BBa_I723020 range2216577 1 2286 2605 annotation2216589 1 BBa_B0015 range2216589 1 3363 3491 annotation2216579 1 BBa_B0030 range2216579 1 2614 2628 annotation2216582 1 BBa_E0040 range2216582 1 2635 3354 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_I723020 1 Pu Pu 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene. false false _126_ 0 2140 9 It's complicated false [Christine/Maia] true Scott Ramsay annotation1955192 1 Pu range1955192 1 1 320 BBa_M1611 1 BBa_M1611 XylR 2013-04-11T11:00:00Z 2015-05-08T01:13:57Z From a complete genomic sequence of Escherichia coli p0305293.5 gecp03052935.contig.229 The MioC Promoter is activated by the presence of DNAa which is produced in the S-Phase of bacterial replication. Isolated from Escherichia coli p0305293.5 gecp03052935.contig.229 false false _768_ 0 16146 9 Not in stock false aaaattttcccc false Nathan Israelsen BBa_I723018 1 Pr Pr (promoter for XylR) 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR). false false _126_ 0 2140 9 Not in stock false [Christine/Maia] false Scott Ramsay annotation1955191 1 Pr range1955191 1 1 410 BBa_M1614_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagattaaagaggagaaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I723020_sequence 1 cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc BBa_M1611_sequence 1 atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatag BBa_B0030_sequence 1 attaaagaggagaaa BBa_M1616_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagattaaagaggagaaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I723018_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z