LOCUS       BBa_M1617               1388 bp    DNA     linear       22-Nov-2024
DEFINITION  loXf+tetP+RBS+cre+ssRa+stop+loXr
ACCESSION   BBa_M1617
VERSION     BBa_M1617.1  
FEATURES             Location/Qualifiers
     misc_feature    1..34
                     /label=BBa_J61046
     misc_feature    43..61
                     /label=TetR 1
     misc_feature    43..96
                     /label=BBa_R0040
     promoter        62..67
                     /label=-35
     misc_feature    68..86
                     /label=TetR 2
     promoter        85..90
                     /label=-10
     promoter        43..96
                     /label=BBa_R0040
     RBS             105..119
                     /label=RBS-1\Strong
     RBS             112..116
                     /label=RBS
     misc_feature    105..119
                     /label=BBa_B0030
     RBS             105..119
                     /label=BBa_B0030
     CDS             126..1162
                     /label=BBa_J61047
     misc_feature    1171..1209
                     /label=AANDENYNYADAS    SsrA deg. tag
     misc_feature    1171..1209
                     /label=BBa_M0051
     stem_loop       1229..1272
                     /label=stem_loop
     misc_feature    1218..1297
                     /label=BBa_B0010
     terminator      1218..1297
                     /label=BBa_B0010
     stem_loop       1313..1332
                     /label=T7 TE
     misc_feature    1339..1339
                     /label=stop
     polyA_site      1333..1346
                     /label=polya
     misc_feature    1306..1346
                     /label=BBa_B0012
     terminator      1306..1346
                     /label=BBa_B0012
     terminator      1218..1346
                     /label=BBa_B0015
     misc_feature    1355..1388
                     /label=BBa_J61046
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattactag agtccctatc agtgatagag
       61 attgacatcc ctatcagtga tagagatact gagcactact agagattaaa gaggagaaat
      121 actagatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
      181 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
      241 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga
      301 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
      361 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
      421 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
      481 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
      541 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
      601 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
      661 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
      721 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
      781 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
      841 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
      901 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
      961 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
     1021 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     1081 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
     1141 tggaagatgg cgattaagaa tttactagag gctgctaacg acgaaaacta caactacgct
     1201 gacgcttctt actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1261 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1321 ttcgggtggg cctttctgcg tttatatact agagataact tcgtataatg tatgctatac
     1381 gaagttat
//