LOCUS BBa_M1618 8289 bp DNA linear 22-Nov-2024 DEFINITION Cell Replication Counter Device ACCESSION BBa_M1618 VERSION BBa_M1618.1 FEATURES Location/Qualifiers misc_feature 1..444 /label=BBa_M1610 promoter 1..444 /label=BBa_M1610 RBS 453..467 /label=paskiba3_rbs CDS 468..1094 /label=paskiba3_TetR misc_feature 453..1094 /label=BBa_I15040 stem_loop 1114..1157 /label=stem_loop misc_feature 1103..1182 /label=BBa_B0010 terminator 1103..1182 /label=BBa_B0010 stem_loop 1198..1217 /label=T7 TE misc_feature 1224..1224 /label=stop polyA_site 1218..1231 /label=polya misc_feature 1191..1231 /label=BBa_B0012 terminator 1191..1231 /label=BBa_B0012 terminator 1103..1231 /label=BBa_B0015 misc_feature 1..1231 /label=BBa_M1612 promoter 1240..1649 /label=Pr promoter 1240..1649 /label=BBa_I723018 RBS 1658..1672 /label=RBS-1\Strong RBS 1665..1669 /label=RBS misc_feature 1658..1672 /label=BBa_B0030 RBS 1658..1672 /label=BBa_B0030 CDS 1679..3379 /label=BBa_M1611 stem_loop 3399..3442 /label=stem_loop misc_feature 3388..3467 /label=BBa_B0010 terminator 3388..3467 /label=BBa_B0010 stem_loop 3483..3502 /label=T7 TE misc_feature 3509..3509 /label=stop polyA_site 3503..3516 /label=polya misc_feature 3476..3516 /label=BBa_B0012 terminator 3476..3516 /label=BBa_B0012 terminator 3388..3516 /label=BBa_B0015 misc_feature 1240..3516 /label=BBa_M1614 promoter 3525..3844 /label=Pu promoter 3525..3844 /label=BBa_I723020 misc_feature 3853..3886 /label=BBa_J61020 misc_feature 3895..3913 /label=TetR 1 misc_feature 3895..3948 /label=BBa_R0040 promoter 3914..3919 /label=-35 misc_feature 3920..3938 /label=TetR 2 promoter 3937..3942 /label=-10 promoter 3895..3948 /label=BBa_R0040 RBS 3957..3971 /label=RBS-1\Strong RBS 3964..3968 /label=RBS misc_feature 3957..3971 /label=BBa_B0030 RBS 3957..3971 /label=BBa_B0030 CDS 3978..5249 /label=BBa_K313002 misc_feature 5258..5296 /label=AANDENYNYADAS SsrA deg. tag misc_feature 5258..5296 /label=BBa_M0051 stem_loop 5316..5359 /label=stem_loop misc_feature 5305..5384 /label=BBa_B0010 terminator 5305..5384 /label=BBa_B0010 stem_loop 5400..5419 /label=T7 TE misc_feature 5426..5426 /label=stop polyA_site 5420..5433 /label=polya misc_feature 5393..5433 /label=BBa_B0012 terminator 5393..5433 /label=BBa_B0012 terminator 5305..5433 /label=BBa_B0015 misc_feature 5442..5475 /label=BBa_J61020 misc_feature 3525..5475 /label=BBa_M1630 misc_feature 5484..5517 /label=BBa_J61046 misc_feature 5526..5544 /label=TetR 1 misc_feature 5526..5579 /label=BBa_R0040 promoter 5545..5550 /label=-35 misc_feature 5551..5569 /label=TetR 2 promoter 5568..5573 /label=-10 promoter 5526..5579 /label=BBa_R0040 RBS 5588..5602 /label=RBS-1\Strong RBS 5595..5599 /label=RBS misc_feature 5588..5602 /label=BBa_B0030 RBS 5588..5602 /label=BBa_B0030 CDS 5609..6645 /label=BBa_J61047 misc_feature 6654..6692 /label=AANDENYNYADAS SsrA deg. tag misc_feature 6654..6692 /label=BBa_M0051 stem_loop 6712..6755 /label=stem_loop misc_feature 6701..6780 /label=BBa_B0010 terminator 6701..6780 /label=BBa_B0010 stem_loop 6796..6815 /label=T7 TE misc_feature 6822..6822 /label=stop polyA_site 6816..6829 /label=polya misc_feature 6789..6829 /label=BBa_B0012 terminator 6789..6829 /label=BBa_B0012 terminator 6701..6829 /label=BBa_B0015 misc_feature 6838..6871 /label=BBa_J61046 misc_feature 5484..6871 /label=BBa_M1617 RBS 6881..6905 /label=wt-RBS misc_feature 6908..7231 /label=antiholin misc_feature 6914..7231 /label=holin misc_feature 7215..7691 /label=endolysin misc_feature 7688..8149 /label=Rz misc_feature 7908..8090 /label=Rz1 misc_feature 6880..8152 /label=BBa_K358004 stem_loop 8172..8215 /label=stem_loop misc_feature 8161..8240 /label=BBa_B0010 terminator 8161..8240 /label=BBa_B0010 stem_loop 8256..8275 /label=T7 TE misc_feature 8282..8282 /label=stop polyA_site 8276..8289 /label=polya misc_feature 8249..8289 /label=BBa_B0012 terminator 8249..8289 /label=BBa_B0012 misc_feature 6880..8289 /label=BBa_K358006 ORIGIN 1 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc 61 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag 121 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc 181 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa 241 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat 301 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc 361 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc 421 gctgataaga gtgatatctg ccattactag agcattggta ggaattaatg atgtctcgtt 481 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 541 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 601 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 661 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 721 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 781 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 841 gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt 901 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 961 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 1021 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 1081 aaagtgggtc ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1201 tcaccttcgg gtgggccttt ctgcgtttat atactagaga ttttaatgtg ggctgcttgg 1261 tgtgatgtag aaaggcgcca agtcgatgaa aatgcatctc gacgtgatgc gtatacgggt 1321 tacccccatt gccacgttgc gccatccttt ttgcaatcag tgaccacttt tccaagcaaa 1381 aataacgcca agcagaacga agacgttctt tttaagaagc gagaacacca gaagttcgtg 1441 ctgtcggggc atgcggcgac gaattggcgg ataaagggga tctgcgttga ggtggatttc 1501 agttaatcaa ttggttaatc tttcaggacc acctaagcaa atgctaaagt ggcagatgga 1561 atgctgagcc ggcaagcaca ggccttgacg ttgcaaggta gtcatgaccg cagtgagcct 1621 ctgatgttcc gccgggtgga tcatcccgat actagagatt aaagaggaga aatactagat 1681 gtcgcttaca tacaaaccca agatgcagca tgaggatatg caagacctta gcagccagat 1741 ccgtttcgtt gccgccgaag gcaagatctg gttgggagag cagcgcatgc tcgtaatgca 1801 gctatctacg ctggccagct tccgtcgcga aattatcagc ttgatcggcg tcgagcgggc 1861 caagggtttc ttcctgcggt tgggctatca gtccggcctg atggatgccg agctggcacg 1921 caagctgcgg ccggccatgc gcgaggagga ggtgttcctg gctgggcctc aattgtatgc 1981 gctcaagggg atggtcaaag tacgcttgct gacaatggat atcgccatcc gggacggacg 2041 tttcaacgtg gaggccgagt ggattgattc ctttgaagtg gatatctgcc gaactgagct 2101 gggcctgatg aatgagcccg tctgctggac ggtgctaggc tatgctagcg gctatggttc 2161 ggcattcatg ggccgcagaa tcattttcca ggaaactagc tgtcgcgggt gcggtgacga 2221 taaatgcctt atcgtcggca agaccgcaga agagtggggc gatgtcagca gtttcgaagc 2281 ctacttcaaa agcgacccga tcgtagacga gcgctacgag ctacagaccc aggttgccaa 2341 cctgcgcaac cgcctgaagc agtacgatgg gcagtattac ggcattggcc attcgccagc 2401 ctacaagcgc atctgtgaga ccatcgacaa ggctgcacgc ggcagggttt cggtcctgct 2461 actgggtgag actggggtgg gcaaggaggt aatcgcgcgc agcgtgcatt tgcgcagtga 2521 gcgcgcagag caacccttcg tcgcggtgaa ctgtgcggca attccgccgg atctgatcga 2581 gtcggaactg tttggtgtcg ataagggcgc ctatacgggc gcggtcaatg cacgcgctgg 2641 acgttttgaa cgggccaacg gcggcaccat ctttcttgat gaggtgatcg aattgacgcc 2701 gagggcccag gccaccctgc tacgggtatt gcaggaagga gagctagagc gggtcggcgg 2761 cgaccgcacg cgaaaggtcg acgtgaggtt aatcaccgca acaaacgaga acctggaaga 2821 ggcggtcaag atggggcgct ttcgcgcaga cctgttcttt cggctgaatg tttttcccgt 2881 gcatatcccg ccgttgcgcg agcgcgtgga agatatcccg ctgctggtcg agcattttct 2941 tagaaggcac cataaggaat acggtaagaa gactcttggc ctgtctgatc gagcgatgga 3001 ggcctgcctc cactaccaat ggccaggcaa tatccgcgag ctggagaacg cccttgagcg 3061 cggggtgatt cttaccgaga gcaacgaaag catcaatgtc gagtcgctgt tcccggggtt 3121 ggcgacggct accgaaggcg acaggctatc gagcgagggc cggttggagg aggagtccgg 3181 tgacagttgg tttaggcaaa ttatcgacca gggcgtcagc ctcgaagatc tcgaagcggg 3241 tttaatgcgc acggccatgg accgttgtgg gcagaatatc tcacaggcgg cgcggttgct 3301 gggattgacc cgcccggcaa tggcctatcg acttaagaag cttgacccca gcttatctgt 3361 gaaagcaatg ggccgatagt actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3421 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3481 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagcccggg aaagcgcgat 3541 gaaccttttt tatcgctgcc ttgatcaaat cgacaggtgg ttatgcgcga ttgatgattt 3601 gctcaaatac agccagcgtg ctgtagattt tctctcatac cccccctttc ttttttacaa 3661 agaaaatcaa taatttagat gaaataaggg gatcggtata agcaatggca tggcggttgc 3721 tagctatacg agacttaaaa taaaaatagt ggtgaccctt caatgttgta ttttctcaac 3781 tctgttcaga ttggttgctt tcgccatgta tatcctcaaa gcgggccagc cgtagccgtt 3841 acgctactag aggaagttcc tatacttttt agagaatagg aacttctact agagtcccta 3901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagatta 3961 aagaggagaa atactagatg agccagtttg gcatattatg taaaacacca cctaaggtcc 4021 tggttcgtca gtttgtggaa aggtttgaaa gaccttcagg ggaaaaaata gcatcatgtg 4081 ctgctgaact aacctattta tgttggatga ttactcataa cggaacagca atcaagagag 4141 ccacattcat gagctataat actatcataa gcaattcgct gagtttcgat attgtcaaca 4201 aatcactcca gtttaaatac aagacgcaaa aagcaacaat tctggaagcc tcattaaaga 4261 aattaattcc tgcttgggaa tttacaatta ttccttacaa tggacaaaaa catcaatctg 4321 atatcactga tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata 4381 agggaaatag ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct 4441 gggagatcac tgagaaaata ctaaattcgt ttgagtatac ctcgagattt acaaaaacaa 4501 aaactttata ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata 4561 ttaagaacgt tgatccgaaa tcatttaaat tagtccaaaa taagtatctg ggagtaataa 4621 tccagtgttt agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg 4681 caaggggtag gatcgatcca cttgtatatt tggatgaatt tttgagaaat tctgaaccag 4741 tcctaaaacg agtaaatagg accggcaatt cttcaagcaa caaacaggaa taccaattat 4801 taaaagataa cttagtcaga tcgtacaaca aggctttgaa gaaaaatgcg ccttatccaa 4861 tctttgctat aaagaatggc ccaaaatctc acattggaag acatttgatg acctcatttc 4921 tgtcaatgaa gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg 4981 cttctgccgt ggccaggaca acgtatactc atcagataac agcaatacct gatcactact 5041 tcgcgctagt ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga 5101 aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg 5161 aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc 5221 tttcatccta cataaataga cgcatataat actagaggct gctaacgacg aaaactacaa 5281 ctacgctgac gcttcttact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 5341 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 5401 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggaagttcct atacttttta 5461 gagaatagga acttctacta gagataactt cgtataatgt atgctatacg aagttattac 5521 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact 5581 actagagatt aaagaggaga aatactagat gtccaattta ctgaccgtac accaaaattt 5641 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt 5701 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc 5761 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt 5821 tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca 5881 tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa 5941 tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc 6001 aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa 6061 tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct 6121 gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg 6181 gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa 6241 ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc 6301 tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc 6361 tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt 6421 gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc 6481 ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca 6541 agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga 6601 aacaggggca atggtgcgcc tgctggaaga tggcgattaa gaatttacta gaggctgcta 6661 acgacgaaaa ctacaactac gctgacgctt cttactagag ccaggcatca aataaaacga 6721 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 6781 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagata 6841 acttcgtata atgtatgcta tacgaagtta ttactagagg agcaaatccc cttattgggg 6901 gtaagacatg aagatgccag aaaaacatga cctgttggcc gccattctcg cggcaaagga 6961 acaaggcatc ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg 7021 cggtgcgttt acaaaaacag taatcgacgc aacgatgtgc gccattatcg cctggttcat 7081 tcgtgacctt ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt 7141 tatcggctac atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa 7201 agccggagta gaagatggta gaaatcaata atcaacgtaa ggcgttcctc gatatgctgg 7261 cgtggtcgga gggaactgat aacggacgtc agaaaaccag aaatcatggt tatgacgtca 7321 ttgtaggcgg agagctattt actgattact ccgatcaccc tcgcaaactt gtcacgctaa 7381 acccaaaact caaatcaaca ggcgccggac gctaccagct tctttcccgt tggtgggatg 7441 cctaccgcaa gcagcttggc ctgaaagact tctctccgaa aagtcaggac gctgtggcat 7501 tgcagcagat taaggagcgt ggcgctttac ctatgattga tcgtggtgat atccgtcagg 7561 caatcgaccg ttgcagcaat atctgggctt cactgccggg cgctggttat ggtcagttcg 7621 agcataaggc tgacagcctg attgcaaaat tcaaagaagc gggcggaacg gtcagagaga 7681 ttgatgtatg agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtctg 7741 cctgtcatgg gctgttaatc attaccgtga taacgccatt acctacaaag cccagcgcga 7801 caaaaatgcc agagaactga agctggcgaa cgcggcaatt actgacatgc agatgcgtca 7861 gcgtgatgtt gctgcgctcg atgcaaaata cacgaaggag ttagctgatg ctaaagctga 7921 aaatgatgct ctgcgtgatg atgttgccgc tggtcgtcgt cggttgcaca tcaaagcagt 7981 ctgtcagtca gtgcgtgaag ccaccaccgc ctccggcgtg gataatgcag cctccccccg 8041 actggcagac accgctgaac gggattattt caccctcaga gagaggctga tcactatgca 8101 aaaacaactg gaaggaaccc agaagtatat taatgagcag tgcagataga gttactagag 8161 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 8221 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 8281 gcgtttata //