BBa_M1618
type: ComponentDefinition
persistent identity: BBa M1618
display id: BBa_M1618
version: 1
was generated by: iGEM to SBOL conversion
description: Cell Replication Counter Device
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: tetP promoter: : BBa_R0040 RBS: BBa_B0030 GFP: BBa_E0040 stop: BBa_B0015
owned by: james
owned by: myers
mutable description: needs to be modified by justin
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: justin
star: false
creator: Justin Hyer
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K358006
component: BBa_M1617
component: BBa_M1630
component: BBa_M1614
component: BBa_M1612
sequence annotation: BBa_M1612
sequence annotation: BBa_M1614
sequence annotation: BBa_M1630
sequence annotation: BBa_M1617
sequence annotation: BBa_K358006
sequence: https://synbiohub.org/public/igem/BBa_M1618_sequence/1

BBa_K358006
type: Component
persistent identity: component2216253
display id: component2216253
version: 1
top level: BBa_M1618
definition: BBa_K358006
access: public

BBa_M1617
type: Component
persistent identity: component2216239
display id: component2216239
version: 1
top level: BBa_M1618
definition: BBa_M1617
access: public

BBa_M1630
type: Component
persistent identity: component2216218
display id: component2216218
version: 1
top level: BBa_M1618
definition: BBa_M1630
access: public

BBa_M1614
type: Component
persistent identity: component2216195
display id: component2216195
version: 1
top level: BBa_M1618
definition: BBa_M1614
access: public

BBa_M1612
type: Component
persistent identity: component2216181
display id: component2216181
version: 1
top level: BBa_M1618
definition: BBa_M1612
access: public

BBa_M1612
type: SequenceAnnotation
persistent identity: annotation2216181
display id: annotation2216181
version: 1
top level: BBa_M1618
location: https://synbiohub.org/public/igem/BBa_M1618/annotation2216181/range2216181/1
component: BBa_M1612

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type: Range
persistent identity: range2216181
display id: range2216181
version: 1
top level: BBa_M1618
direction: direction/forward
start: 1
end: 1231
orientation: inline

BBa_M1614
type: SequenceAnnotation
persistent identity: annotation2216195
display id: annotation2216195
version: 1
top level: BBa_M1618
location: https://synbiohub.org/public/igem/BBa_M1618/annotation2216195/range2216195/1
component: BBa_M1614

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type: Range
persistent identity: range2216195
display id: range2216195
version: 1
top level: BBa_M1618
direction: direction/reverse
start: 1240
end: 3516
orientation: inline

BBa_M1630
type: SequenceAnnotation
persistent identity: annotation2216218
display id: annotation2216218
version: 1
top level: BBa_M1618
location: https://synbiohub.org/public/igem/BBa_M1618/annotation2216218/range2216218/1
component: BBa_M1630

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type: Range
persistent identity: range2216218
display id: range2216218
version: 1
top level: BBa_M1618
direction: direction/reverse
start: 3525
end: 5475
orientation: inline

BBa_M1617
type: SequenceAnnotation
persistent identity: annotation2216239
display id: annotation2216239
version: 1
top level: BBa_M1618
location: https://synbiohub.org/public/igem/BBa_M1618/annotation2216239/range2216239/1
component: BBa_M1617

https://synbiohub.org/public/igem/BBa_M1618/annotation2216239/range2216239/1
type: Range
persistent identity: range2216239
display id: range2216239
version: 1
top level: BBa_M1618
direction: direction/reverse
start: 5484
end: 6871
orientation: inline

BBa_K358006
type: SequenceAnnotation
persistent identity: annotation2216253
display id: annotation2216253
version: 1
top level: BBa_M1618
location: https://synbiohub.org/public/igem/BBa_M1618/annotation2216253/range2216253/1
component: BBa_K358006

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type: Range
persistent identity: range2216253
display id: range2216253
version: 1
top level: BBa_M1618
direction: direction/reverse
start: 6880
end: 8289
orientation: inline

BBa_M1612
type: ComponentDefinition
persistent identity: BBa M1612
display id: BBa_M1612
version: 1
was generated by: iGEM to SBOL conversion
description: mioC promoter + RBS + tetR (standard 10 compatible)
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: mioC: Escherichia coli p0305293.5 gecp03052935.contig.229, whole genome shotgun sequence. RBS + tetR: BBa_I15040
owned by: james
owned by: myers
mutable description: The MioC promoter is a cell-cycle dependent promoter. Nathan knows this!!! he will modified
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16130
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard 10 compatible
star: false
creator: Maureen Kesaano
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_I15040
component: BBa_M1610
sequence annotation: BBa_M1610
sequence annotation: BBa_I15040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1612_sequence/1

BBa_B0015
type: Component
persistent identity: component2215714
display id: component2215714
version: 1
top level: BBa_M1612
definition: BBa_B0015
access: public

BBa_I15040
type: Component
persistent identity: component2215707
display id: component2215707
version: 1
top level: BBa_M1612
definition: RBS.TetR
access: public

BBa_M1610
type: Component
persistent identity: component2215703
display id: component2215703
version: 1
top level: BBa_M1612
definition: BBa_M1610
access: public

BBa_M1610
type: SequenceAnnotation
persistent identity: annotation2215703
display id: annotation2215703
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215703/range2215703/1
component: BBa_M1610

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type: Range
persistent identity: range2215703
display id: range2215703
version: 1
top level: BBa_M1612
direction: direction/forward
start: 1
end: 444
orientation: inline

BBa_I15040
type: SequenceAnnotation
persistent identity: annotation2215707
display id: annotation2215707
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215707/range2215707/1
component: BBa_I15040

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type: Range
persistent identity: range2215707
display id: range2215707
version: 1
top level: BBa_M1612
direction: direction/reverse
start: 453
end: 1094
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2215714
display id: annotation2215714
version: 1
top level: BBa_M1612
location: https://synbiohub.org/public/igem/BBa_M1612/annotation2215714/range2215714/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1612/annotation2215714/range2215714/1
type: Range
persistent identity: range2215714
display id: range2215714
version: 1
top level: BBa_M1612
direction: direction/reverse
start: 1103
end: 1231
orientation: inline

BBa_M1614
type: ComponentDefinition
persistent identity: BBa M1614
display id: BBa_M1614
version: 1
was generated by: iGEM to SBOL conversion
description: Pr promoter+RBS+ XylR + terminator
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Pr: BBa_I723018 xylR: BBa_M1611 Pu:BBa_I723020
owned by: james
owned by: myers
mutable description: eeeee
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16142
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standar 10 compatible
star: false
creator: Kandy Napan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1611
component: BBa_B0030
component: BBa_I723018
component: BBa_B0015
sequence annotation: BBa_I723018
sequence annotation: BBa_B0030
sequence annotation: BBa_M1611
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1614_sequence/1

BBa_M1611
type: Component
persistent identity: component2215989
display id: component2215989
version: 1
top level: BBa_M1614
definition: BBa_M1611
access: public

BBa_B0030
type: Component
persistent identity: component2215987
display id: component2215987
version: 1
top level: BBa_M1614
definition: BBa_B0030
access: public

BBa_I723018
type: Component
persistent identity: component2215985
display id: component2215985
version: 1
top level: BBa_M1614
definition: Pr
access: public

BBa_B0015
type: Component
persistent identity: component2215996
display id: component2215996
version: 1
top level: BBa_M1614
definition: BBa_B0015
access: public

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2215985
display id: annotation2215985
version: 1
top level: BBa_M1614
location: https://synbiohub.org/public/igem/BBa_M1614/annotation2215985/range2215985/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_M1614/annotation2215985/range2215985/1
type: Range
persistent identity: range2215985
display id: range2215985
version: 1
top level: BBa_M1614
direction: direction/reverse
start: 1
end: 410
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2215987
display id: annotation2215987
version: 1
top level: BBa_M1614
location: https://synbiohub.org/public/igem/BBa_M1614/annotation2215987/range2215987/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1614/annotation2215987/range2215987/1
type: Range
persistent identity: range2215987
display id: range2215987
version: 1
top level: BBa_M1614
direction: direction/forward
start: 419
end: 433
orientation: inline

BBa_M1611
type: SequenceAnnotation
persistent identity: annotation2215989
display id: annotation2215989
version: 1
top level: BBa_M1614
location: https://synbiohub.org/public/igem/BBa_M1614/annotation2215989/range2215989/1
component: BBa_M1611

https://synbiohub.org/public/igem/BBa_M1614/annotation2215989/range2215989/1
type: Range
persistent identity: range2215989
display id: range2215989
version: 1
top level: BBa_M1614
direction: direction/reverse
start: 440
end: 2140
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2215996
display id: annotation2215996
version: 1
top level: BBa_M1614
location: https://synbiohub.org/public/igem/BBa_M1614/annotation2215996/range2215996/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1614/annotation2215996/range2215996/1
type: Range
persistent identity: range2215996
display id: range2215996
version: 1
top level: BBa_M1614
direction: direction/reverse
start: 2149
end: 2277
orientation: inline

BBa_M1630
type: ComponentDefinition
persistent identity: BBa M1630
display id: BBa_M1630
version: 1
was generated by: iGEM to SBOL conversion
description: mioC activated tet R +terminator
created: 2013-04-14T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: combination Biobrick
owned by: james
owned by: myers
mutable description: tet R coding sequence combined with mioC promoter to allow production of tet R protein during cell replication.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 17020
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: standard ten compatible
star: false
creator: kun zhang
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_J61020
component: BBa_B0015
component: BBa_M0051
component: BBa_K313002
component: BBa_B0030
component: BBa_R0040
component: BBa_I723020
sequence annotation: BBa_I723020
sequence annotation: BBa_J61020
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_K313002
sequence annotation: BBa_M0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1630_sequence/1

BBa_J61020
type: Component
persistent identity: component2215809
display id: component2215809
version: 1
top level: BBa_M1630
definition: FRT
access: public

BBa_B0015
type: Component
persistent identity: component2215808
display id: component2215808
version: 1
top level: BBa_M1630
definition: BBa_B0015
access: public

BBa_M0051
type: Component
persistent identity: component2215801
display id: component2215801
version: 1
top level: BBa_M1630
definition: BBa_M0051
access: public

BBa_K313002
type: Component
persistent identity: component2215799
display id: component2215799
version: 1
top level: BBa_M1630
definition: BBa_K313002
access: public

BBa_B0030
type: Component
persistent identity: component2215797
display id: component2215797
version: 1
top level: BBa_M1630
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component2215791
display id: component2215791
version: 1
top level: BBa_M1630
definition: p(tetR)
access: public

BBa_J61020
type: Component
persistent identity: component2215790
display id: component2215790
version: 1
top level: BBa_M1630
definition: FRT
access: public

BBa_I723020
type: Component
persistent identity: component2215789
display id: component2215789
version: 1
top level: BBa_M1630
definition: Pu
access: public

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2215789
display id: annotation2215789
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215789/range2215789/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_M1630/annotation2215789/range2215789/1
type: Range
persistent identity: range2215789
display id: range2215789
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 1
end: 320
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation2215790
display id: annotation2215790
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215790/range2215790/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_M1630/annotation2215790/range2215790/1
type: Range
persistent identity: range2215790
display id: range2215790
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 329
end: 362
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2215791
display id: annotation2215791
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215791/range2215791/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1630/annotation2215791/range2215791/1
type: Range
persistent identity: range2215791
display id: range2215791
version: 1
top level: BBa_M1630
direction: direction/forward
start: 371
end: 424
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2215797
display id: annotation2215797
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215797/range2215797/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1630/annotation2215797/range2215797/1
type: Range
persistent identity: range2215797
display id: range2215797
version: 1
top level: BBa_M1630
direction: direction/forward
start: 433
end: 447
orientation: inline

BBa_K313002
type: SequenceAnnotation
persistent identity: annotation2215799
display id: annotation2215799
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215799/range2215799/1
component: BBa_K313002

https://synbiohub.org/public/igem/BBa_M1630/annotation2215799/range2215799/1
type: Range
persistent identity: range2215799
display id: range2215799
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 454
end: 1725
orientation: inline

BBa_M0051
type: SequenceAnnotation
persistent identity: annotation2215801
display id: annotation2215801
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215801/range2215801/1
component: BBa_M0051

https://synbiohub.org/public/igem/BBa_M1630/annotation2215801/range2215801/1
type: Range
persistent identity: range2215801
display id: range2215801
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 1734
end: 1772
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2215808
display id: annotation2215808
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215808/range2215808/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1630/annotation2215808/range2215808/1
type: Range
persistent identity: range2215808
display id: range2215808
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 1781
end: 1909
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation2215809
display id: annotation2215809
version: 1
top level: BBa_M1630
location: https://synbiohub.org/public/igem/BBa_M1630/annotation2215809/range2215809/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_M1630/annotation2215809/range2215809/1
type: Range
persistent identity: range2215809
display id: range2215809
version: 1
top level: BBa_M1630
direction: direction/reverse
start: 1918
end: 1951
orientation: inline

BBa_M1617
type: ComponentDefinition
persistent identity: BBa M1617
display id: BBa_M1617
version: 1
was generated by: iGEM to SBOL conversion
description: loXf+tetP+RBS+cre+ssRa+stop+loXr
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: needs to be modified by Justin
owned by: james
owned by: myers
mutable description: loXf: BBa_J61046 tetP promoter reverse: BBa_R0040 RBS: BBa_B0030 cre: BBa_J61047 ssRa: BBa_K313002 stop: BBa_B0015 loXr: BBa_J61046
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standard 10 compatible.
star: false
creator: Justin Hyer
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_J61046
component: BBa_M0051
component: BBa_J61047
component: BBa_B0030
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_J61046
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J61047
sequence annotation: BBa_M0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1617_sequence/1

BBa_J61046
type: Component
persistent identity: component2216590
display id: component2216590
version: 1
top level: BBa_M1617
definition: lox
access: public

BBa_M0051
type: Component
persistent identity: component2216601
display id: component2216601
version: 1
top level: BBa_M1617
definition: BBa_M0051
access: public

BBa_J61047
type: Component
persistent identity: component2216599
display id: component2216599
version: 1
top level: BBa_M1617
definition: Cre
access: public

BBa_B0030
type: Component
persistent identity: component2216597
display id: component2216597
version: 1
top level: BBa_M1617
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component2216591
display id: component2216591
version: 1
top level: BBa_M1617
definition: p(tetR)
access: public

BBa_J61046
type: Component
persistent identity: component2216609
display id: component2216609
version: 1
top level: BBa_M1617
definition: lox
access: public

BBa_B0015
type: Component
persistent identity: component2216608
display id: component2216608
version: 1
top level: BBa_M1617
definition: BBa_B0015
access: public

BBa_J61046
type: SequenceAnnotation
persistent identity: annotation2216590
display id: annotation2216590
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216590/range2216590/1
component: BBa_J61046

https://synbiohub.org/public/igem/BBa_M1617/annotation2216590/range2216590/1
type: Range
persistent identity: range2216590
display id: range2216590
version: 1
top level: BBa_M1617
direction: direction/reverse
start: 1
end: 34
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2216591
display id: annotation2216591
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216591/range2216591/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1617/annotation2216591/range2216591/1
type: Range
persistent identity: range2216591
display id: range2216591
version: 1
top level: BBa_M1617
direction: direction/forward
start: 43
end: 96
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2216597
display id: annotation2216597
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216597/range2216597/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1617/annotation2216597/range2216597/1
type: Range
persistent identity: range2216597
display id: range2216597
version: 1
top level: BBa_M1617
direction: direction/forward
start: 105
end: 119
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2216599
display id: annotation2216599
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216599/range2216599/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_M1617/annotation2216599/range2216599/1
type: Range
persistent identity: range2216599
display id: range2216599
version: 1
top level: BBa_M1617
direction: direction/reverse
start: 126
end: 1162
orientation: inline

BBa_M0051
type: SequenceAnnotation
persistent identity: annotation2216601
display id: annotation2216601
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216601/range2216601/1
component: BBa_M0051

https://synbiohub.org/public/igem/BBa_M1617/annotation2216601/range2216601/1
type: Range
persistent identity: range2216601
display id: range2216601
version: 1
top level: BBa_M1617
direction: direction/reverse
start: 1171
end: 1209
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2216608
display id: annotation2216608
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216608/range2216608/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1617/annotation2216608/range2216608/1
type: Range
persistent identity: range2216608
display id: range2216608
version: 1
top level: BBa_M1617
direction: direction/reverse
start: 1218
end: 1346
orientation: inline

BBa_J61046
type: SequenceAnnotation
persistent identity: annotation2216609
display id: annotation2216609
version: 1
top level: BBa_M1617
location: https://synbiohub.org/public/igem/BBa_M1617/annotation2216609/range2216609/1
component: BBa_J61046

https://synbiohub.org/public/igem/BBa_M1617/annotation2216609/range2216609/1
type: Range
persistent identity: range2216609
display id: range2216609
version: 1
top level: BBa_M1617
direction: direction/reverse
start: 1355
end: 1388
orientation: inline

BBa_K358006
type: ComponentDefinition
persistent identity: BBa K358006
display id: BBa_K358006
version: 1
was generated by: iGEM to SBOL conversion
description: lambda lysis cassette with terminator
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: BBa_K358004 and BBa_B0015
owned by: james
owned by: myers
mutable description: Lambda lysis cassette[BBa_K358004] with double terminator[BBa_B0015].
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: non
star: false
creator: Ken Kajita
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_K358004
sequence annotation: BBa_K358004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K358006_sequence/1

BBa_B0012
type: Component
persistent identity: component2080474
display id: component2080474
version: 1
top level: BBa_K358006
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2080472
display id: component2080472
version: 1
top level: BBa_K358006
definition: BBa_B0010
access: public

BBa_K358004
type: Component
persistent identity: component2080471
display id: component2080471
version: 1
top level: BBa_K358006
definition: BBa_K358004
access: public

BBa_K358004
type: SequenceAnnotation
persistent identity: annotation2080471
display id: annotation2080471
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080471/range2080471/1
component: BBa_K358004

https://synbiohub.org/public/igem/BBa_K358006/annotation2080471/range2080471/1
type: Range
persistent identity: range2080471
display id: range2080471
version: 1
top level: BBa_K358006
direction: direction/reverse
start: 1
end: 1273
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2080472
display id: annotation2080472
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080472/range2080472/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K358006/annotation2080472/range2080472/1
type: Range
persistent identity: range2080472
display id: range2080472
version: 1
top level: BBa_K358006
direction: direction/forward
start: 1282
end: 1361
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2080474
display id: annotation2080474
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080474/range2080474/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K358006/annotation2080474/range2080474/1
type: Range
persistent identity: range2080474
display id: range2080474
version: 1
top level: BBa_K358006
direction: direction/forward
start: 1370
end: 1410
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

Pr
type: ComponentDefinition
persistent identity: BBa I723018
display id: BBa_I723018
version: 1
was generated by: iGEM to SBOL conversion
description: Pr (promoter for XylR)
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: [Christine/Maia]
star: false
creator: Scott Ramsay
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pr
sequence: https://synbiohub.org/public/igem/BBa_I723018_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation1955191
display id: annotation1955191
version: 1
top level: Pr
location: https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
type: Range
persistent identity: range1955191
display id: range1955191
version: 1
top level: Pr
direction: direction/forward
start: 1
end: 410
orientation: inline

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: iGEM to SBOL conversion
description: Pu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: [Christine/Maia]
star: true
creator: Scott Ramsay
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
top level: Pu
direction: direction/forward
start: 1
end: 320
orientation: inline

BBa_M0051
type: ComponentDefinition
persistent identity: BBa M0051
display id: BBa_M0051
version: 1
was generated by: iGEM to SBOL conversion
description: AANDENYNYADAS. (Fast) SsrA degradation tag.
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: This variation of the SsrA tag was studied by McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701.
owned by: james
owned by: myers
mutable description: This sequence codes for the amino acid sequence AANDENYNYADAS, a variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~2 Vmax/ [Clpx6] min-1 from (1). See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: C-terminal tag. Degradation rate is fast. Variations of this sequence yield different degradation rates(see Parts BBa_M0050, BBa_M0052, BBa_M0053). Sequence derived from reverse translation of AANDENYNYADAS sequence using codon usage optimized for E. coli. Three C-terminal aa's (DAS in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system. NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to inlclude them if you use this sequence.
star: false
creator: Felix Moser
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: AANDENYNYADAS SsrA deg. tag
sequence: https://synbiohub.org/public/igem/BBa_M0051_sequence/1

AANDENYNYADAS SsrA deg. tag
type: SequenceAnnotation
persistent identity: annotation1958881
display id: annotation1958881
version: 1
top level: BBa_M0051
location: https://synbiohub.org/public/igem/BBa_M0051/annotation1958881/range1958881/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_M0051/annotation1958881/range1958881/1
type: Range
persistent identity: range1958881
display id: range1958881
version: 1
top level: BBa_M0051
direction: direction/forward
start: 1
end: 39
orientation: inline

BBa_M1610
type: ComponentDefinition
persistent identity: BBa M1610
display id: BBa_M1610
version: 1
was generated by: iGEM to SBOL conversion
description: MioC Promoter
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: From a complete genomic sequence of Escherichia coli p0305293.5 gecp03052935.contig.229
owned by: james
owned by: myers
mutable description: The MioC Promoter is activated by the presence of DNAa which is produced in the S-Phase of bacterial replication. Isolated from Escherichia coli p0305293.5 gecp03052935.contig.229
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16146
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standard 10 compatible
star: false
creator: Nathan Israelsen
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_M1610
sequence: https://synbiohub.org/public/igem/BBa_M1610_sequence/1

BBa_M1610
type: SequenceAnnotation
persistent identity: annotation2214866
display id: annotation2214866
version: 1
top level: BBa_M1610
location: https://synbiohub.org/public/igem/BBa_M1610/annotation2214866/range2214866/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1610/annotation2214866/range2214866/1
type: Range
persistent identity: range2214866
display id: range2214866
version: 1
top level: BBa_M1610
direction: direction/forward
start: 1
end: 444
orientation: inline

BBa_M1611
type: ComponentDefinition
persistent identity: BBa M1611
display id: BBa_M1611
version: 1
was generated by: iGEM to SBOL conversion
description: XylR
created: 2013-04-11T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: From a complete genomic sequence of Escherichia coli p0305293.5 gecp03052935.contig.229
owned by: james
owned by: myers
mutable description: The MioC Promoter is activated by the presence of DNAa which is produced in the S-Phase of bacterial replication. Isolated from Escherichia coli p0305293.5 gecp03052935.contig.229
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16146
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: aaaattttcccc
star: false
creator: Nathan Israelsen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M1611_sequence/1

BBa_K313002
type: ComponentDefinition
persistent identity: BBa K313002
display id: BBa_K313002
version: 1
was generated by: iGEM to SBOL conversion
description: flpe DNA recombinase
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:11:54Z
mutable provenance: E.coli EL250 strain
owned by: james
owned by: myers
mutable description: Flpe is a site-specific DNA recombinase that that recognizes FRT sequence. Flpe was derived from yeaset site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 )) It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
discontinued: false
dominant: false
experience: experience/None
group u list: _433_
m user id: 0
owner id: 5929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
star: false
creator: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K313002_sequence/1

FRT
type: ComponentDefinition
persistent identity: BBa J61020
display id: BBa_J61020
version: 1
was generated by: iGEM to SBOL conversion
description: [FRT]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Extend ca1010F/R (73 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is pSB1A2-Bca1010 ---- ca1010F Forward (universal biobrick) EcoRI for FRT GGACTgaattcgcggccgcttctagag ca1010R Reverse SpeI oligo for FRT cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg
owned by: james
owned by: myers
mutable description: Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61020_sequence/1

RBS.TetR
type: ComponentDefinition
persistent identity: BBa I15040
display id: BBa_I15040
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.TetR from pASKIBA3
created: 2005-06-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: pASKIBA3 (IBA biotagnology)
owned by: james
owned by: myers
mutable description: The part accepts POPS to produce TetR. This sequence encodes the ribosome binding site and tetracycline repressor protein from the plasmid pASKIBA3.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: paskiba3_rbs
sequence annotation: paskiba3_TetR
sequence: https://synbiohub.org/public/igem/BBa_I15040_sequence/1

paskiba3_rbs
type: SequenceAnnotation
persistent identity: annotation1501145
display id: annotation1501145
version: 1
top level: RBS.TetR
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
type: Range
persistent identity: range1501145
display id: range1501145
version: 1
top level: RBS.TetR
direction: direction/forward
start: 1
end: 15
orientation: inline

paskiba3_TetR
type: SequenceAnnotation
persistent identity: annotation1501146
display id: annotation1501146
version: 1
top level: RBS.TetR
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
type: Range
persistent identity: range1501146
display id: range1501146
version: 1
top level: RBS.TetR
direction: direction/forward
start: 16
end: 642
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

Cre
type: ComponentDefinition
persistent identity: BBa J61047
display id: BBa_J61047
version: 1
was generated by: iGEM to SBOL conversion
description: Cre DNA recombinase
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61047_sequence/1

lox
type: ComponentDefinition
persistent identity: BBa J61046
display id: BBa_J61046
version: 1
was generated by: iGEM to SBOL conversion
description: [Lox] site for recombination
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61046_sequence/1

BBa_K358004
type: ComponentDefinition
persistent identity: BBa K358004
display id: BBa_K358004
version: 1
was generated by: iGEM to SBOL conversion
description: lambda lysis cassette[SRRz/Rz1]
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: λ -Hind Ⅲ digest (product of TaKaRa Bio)
owned by: james
owned by: myers
mutable description: This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We made point mutation and prepared wild type phage DNA.
star: false
creator: Ken Kajita
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: wt-RBS
sequence annotation: antiholin
sequence annotation: holin
sequence annotation: endolysin
sequence annotation: Rz
sequence annotation: Rz1
sequence: https://synbiohub.org/public/igem/BBa_K358004_sequence/1

wt-RBS
type: SequenceAnnotation
persistent identity: annotation2080446
display id: annotation2080446
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1
type: Range
persistent identity: range2080446
display id: range2080446
version: 1
top level: BBa_K358004
direction: direction/forward
start: 2
end: 26
orientation: inline

antiholin
type: SequenceAnnotation
persistent identity: annotation2080447
display id: annotation2080447
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1
type: Range
persistent identity: range2080447
display id: range2080447
version: 1
top level: BBa_K358004
direction: direction/forward
start: 29
end: 352
orientation: inline

holin
type: SequenceAnnotation
persistent identity: annotation2080448
display id: annotation2080448
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1
type: Range
persistent identity: range2080448
display id: range2080448
version: 1
top level: BBa_K358004
direction: direction/forward
start: 35
end: 352
orientation: inline

endolysin
type: SequenceAnnotation
persistent identity: annotation2080449
display id: annotation2080449
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1
type: Range
persistent identity: range2080449
display id: range2080449
version: 1
top level: BBa_K358004
direction: direction/forward
start: 336
end: 812
orientation: inline

Rz
type: SequenceAnnotation
persistent identity: annotation2080450
display id: annotation2080450
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1
type: Range
persistent identity: range2080450
display id: range2080450
version: 1
top level: BBa_K358004
direction: direction/forward
start: 809
end: 1270
orientation: inline

Rz1
type: SequenceAnnotation
persistent identity: annotation2080451
display id: annotation2080451
version: 1
top level: BBa_K358004
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1
type: Range
persistent identity: range2080451
display id: range2080451
version: 1
top level: BBa_K358004
direction: direction/forward
start: 1029
end: 1211
orientation: inline

https://synbiohub.org/public/igem/BBa_M1618_sequence/1
type: Sequence
persistent identity: BBa M1618 sequence
display id: BBa_M1618_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccattactagagcattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagattaaagaggagaaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1612_sequence/1
type: Sequence
persistent identity: BBa M1612 sequence
display id: BBa_M1612_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccattactagagcattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1614_sequence/1
type: Sequence
persistent identity: BBa M1614 sequence
display id: BBa_M1614_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagattaaagaggagaaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1630_sequence/1
type: Sequence
persistent identity: BBa M1630 sequence
display id: BBa_M1630_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagaggaagttcctatactttttagagaataggaacttctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataatactagaggctgctaacgacgaaaactacaactacgctgacgcttcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggaagttcctatactttttagagaataggaacttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1617_sequence/1
type: Sequence
persistent identity: BBa M1617 sequence
display id: BBa_M1617_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttattactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatttactagaggctgctaacgacgaaaactacaactacgctgacgcttcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K358006_sequence/1
type: Sequence
persistent identity: BBa K358006 sequence
display id: BBa_K358006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723018_sequence/1
type: Sequence
persistent identity: BBa I723018 sequence
display id: BBa_I723018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723020_sequence/1
type: Sequence
persistent identity: BBa I723020 sequence
display id: BBa_I723020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M0051_sequence/1
type: Sequence
persistent identity: BBa M0051 sequence
display id: BBa_M0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgctaacgacgaaaactacaactacgctgacgcttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1610_sequence/1
type: Sequence
persistent identity: BBa M1610 sequence
display id: BBa_M1610_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatcttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgtcatattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcatagaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaatcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacatattcggcaccgccgagggtgctgccgctgataagagtgatatctgccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1611_sequence/1
type: Sequence
persistent identity: BBa M1611 sequence
display id: BBa_M1611_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctacagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K313002_sequence/1
type: Sequence
persistent identity: BBa K313002 sequence
display id: BBa_K313002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61020_sequence/1
type: Sequence
persistent identity: BBa J61020 sequence
display id: BBa_J61020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctatactttttagagaataggaacttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15040_sequence/1
type: Sequence
persistent identity: BBa I15040 sequence
display id: BBa_I15040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61047_sequence/1
type: Sequence
persistent identity: BBa J61047 sequence
display id: BBa_J61047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61046_sequence/1
type: Sequence
persistent identity: BBa J61046 sequence
display id: BBa_J61046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K358004_sequence/1
type: Sequence
persistent identity: BBa K358004 sequence
display id: BBa_K358004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z