LOCUS       BBa_M1630               1951 bp    DNA     linear       22-Nov-2024
DEFINITION  mioC activated tet R +terminator
ACCESSION   BBa_M1630
VERSION     BBa_M1630.1  
FEATURES             Location/Qualifiers
     promoter        1..320
                     /label=Pu
     promoter        1..320
                     /label=BBa_I723020
     misc_feature    329..362
                     /label=BBa_J61020
     misc_feature    371..389
                     /label=TetR 1
     misc_feature    371..424
                     /label=BBa_R0040
     promoter        390..395
                     /label=-35
     misc_feature    396..414
                     /label=TetR 2
     promoter        413..418
                     /label=-10
     promoter        371..424
                     /label=BBa_R0040
     RBS             433..447
                     /label=RBS-1\Strong
     RBS             440..444
                     /label=RBS
     misc_feature    433..447
                     /label=BBa_B0030
     RBS             433..447
                     /label=BBa_B0030
     CDS             454..1725
                     /label=BBa_K313002
     misc_feature    1734..1772
                     /label=AANDENYNYADAS    SsrA deg. tag
     misc_feature    1734..1772
                     /label=BBa_M0051
     stem_loop       1792..1835
                     /label=stem_loop
     misc_feature    1781..1860
                     /label=BBa_B0010
     terminator      1781..1860
                     /label=BBa_B0010
     stem_loop       1876..1895
                     /label=T7 TE
     misc_feature    1902..1902
                     /label=stop
     polyA_site      1896..1909
                     /label=polya
     misc_feature    1869..1909
                     /label=BBa_B0012
     terminator      1869..1909
                     /label=BBa_B0012
     terminator      1781..1909
                     /label=BBa_B0015
     misc_feature    1918..1951
                     /label=BBa_J61020
ORIGIN
        1 cccgggaaag cgcgatgaac cttttttatc gctgccttga tcaaatcgac aggtggttat
       61 gcgcgattga tgatttgctc aaatacagcc agcgtgctgt agattttctc tcataccccc
      121 cctttctttt ttacaaagaa aatcaataat ttagatgaaa taaggggatc ggtataagca
      181 atggcatggc ggttgctagc tatacgagac ttaaaataaa aatagtggtg acccttcaat
      241 gttgtatttt ctcaactctg ttcagattgg ttgctttcgc catgtatatc ctcaaagcgg
      301 gccagccgta gccgttacgc tactagagga agttcctata ctttttagag aataggaact
      361 tctactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      421 gcactactag agattaaaga ggagaaatac tagatgagcc agtttggcat attatgtaaa
      481 acaccaccta aggtcctggt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggggaa
      541 aaaatagcat catgtgctgc tgaactaacc tatttatgtt ggatgattac tcataacgga
      601 acagcaatca agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt
      661 ttcgatattg tcaacaaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg
      721 gaagcctcat taaagaaatt aattcctgct tgggaattta caattattcc ttacaatgga
      781 caaaaacatc aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca
      841 tcggaagaag cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt
      901 gagggtgaaa gcatctggga gatcactgag aaaatactaa attcgtttga gtatacctcg
      961 agatttacaa aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt
     1021 ggaagattca gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag
     1081 tatctgggag taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac
     1141 atatacttct ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg
     1201 agaaattctg aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaacaaa
     1261 caggaatacc aattattaaa agataactta gtcagatcgt acaacaaggc tttgaagaaa
     1321 aatgcgcctt atccaatctt tgctataaag aatggcccaa aatctcacat tggaagacat
     1381 ttgatgacct catttctgtc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat
     1441 tggagcgata agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca
     1501 atacctgatc actacttcgc gctagtttct cggtactatg catatgatcc aatatcaaag
     1561 gaaatgatag cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag
     1621 ctaaagggta gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag
     1681 gaggtactag actacctttc atcctacata aatagacgca tataatacta gaggctgcta
     1741 acgacgaaaa ctacaactac gctgacgctt cttactagag ccaggcatca aataaaacga
     1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1861 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagaggaa
     1921 gttcctatac tttttagaga ataggaactt c
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