##gff-version 3
##sequence-region BBa_M1630 1 1951
BBa_M1630	.	sequence_feature	1918	1951	.	+	0	ID=BBa_J61020;Name=BBa_J61020
BBa_M1630	.	promoter	1	320	.	+	0	ID=BBa_I723020;Name=BBa_I723020
BBa_M1630	.	promoter	1	320	.	+	0	ID=annotation1955192;Name=Pu;Parent=BBa_I723020
BBa_M1630	.	terminator	1781	1909	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1630	.	terminator	1781	1860	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1630	.	engineered_region	1781	1860	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1630	.	stem_loop	1792	1835	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1630	.	terminator	1869	1909	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1630	.	stem_loop	1876	1895	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1630	.	polyA_site	1896	1909	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1630	.	stop_codon	1902	1902	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1630	.	engineered_region	1869	1909	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1630	.	CDS	454	1725	.	+	0	ID=BBa_K313002;Name=BBa_K313002
BBa_M1630	.	ribosome_entry_site	433	447	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1630	.	ribosome_entry_site	433	447	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1630	.	ribosome_entry_site	440	444	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1630	.	engineered_region	433	447	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1630	.	sequence_feature	329	362	.	+	0	ID=BBa_J61020;Name=BBa_J61020
BBa_M1630	.	tag	1734	1772	.	+	0	ID=BBa_M0051;Name=BBa_M0051
BBa_M1630	.	tag	1734	1772	.	+	0	ID=annotation1958881;Name=AANDENYNYADAS    SsrA deg. tag;Parent=BBa_M0051
BBa_M1630	.	promoter	371	424	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_M1630	.	promoter	390	395	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1630	.	promoter	413	418	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1630	.	non_covalent_binding_site	371	389	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1630	.	non_covalent_binding_site	396	414	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1630	.	engineered_region	371	424	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_M1630
cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgct
caaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatga
aataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttc
aatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgtt
acgctactagaggaagttcctatactttttagagaataggaacttctactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgagccagtttggcatatta
tgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcat
gtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagcta
taatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagca
acaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatc
aatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagcca
cagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgttt
gagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagat
tcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgttt
agtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatat
ttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacagg
aataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgc
tataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttg
actaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaa
tacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaagga
tgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgca
tggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataatactagaggctgc
taacgacgaaaactacaactacgctgacgcttcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagaggaagttcctatactttttagagaataggaacttc