BBa_M1631
1
BBa_M1631
Mutated lacI 1
2013-04-11T11:00:00Z
2015-05-08T01:13:57Z
LacI amino acid sequence with H2 binding-related amino acids, as discussed in www.sciencemag.org/cgi/content/321/5888/572/DC1 (GenBank: AAB98710.1)
lacI was mutated by removing the lactose binding site (AA 75,76,79)and replaced by hydrogen binding site (His, His, Cys)
false
false
_768_
0
16147
9
Not in stock
false
Back translated and optimized to remove standard restriction sites
false
Oumou Diallo
annotation2214850
1
Codes for H2 binding site
range2214850
1
223
237
BBa_M1631_sequence
1
atgaaacctgtgactctgtatgatgttgcggagtatgcaggtgtctcgtaccagaccgtgagccgcgttgttaaccaagctagtcatgtcagcgcgaaaacgcgtgagaaagtggaagctgcaatggcggaattgaactacattccaaatcgtgtcgcacagcaactagcgggcaaacaatctcttctgataggcgtagctacgagttctctggcattgcaccatcactcccaatgtgttgcagcgataaagtcacgtgcagatcaattgggtgcgtcggtggtcgtgtctatggtggaaagatcaggtgtggaagcatgcaaagcagcggttcataatctgctggcacaacgtgtatcaggactactgattaactatccccttgacgatcaagatgcgacggccgttgaagccgcatgcgctaatgtccctgcccttttcttagacgttagtgatcaaactccgatcaattcaatcatttttagccatgaggatggcactcggcttggcgtagagcacttagtagcgttgggacatcaacagattgctcttttggcgggcccattaagcagtgtgtcagcgcgtttgcgactagccggctggcacaaatatttgacccgcaaccaaatccagccgattgctgagcgggagggcgattggtcggcaatgtctggctttcagcaaacgatgcaaatgctgaatgaaggaatcgtcccaactgcaatgctggtggctaacgatcagatggccttaggcgcgatgcgtgcaatcaccgagtctggactgcgggtgggagcagatatatcggtcgttgggtatgatgatacagaagactcttcatgttacattccgccgctgactacgatcaaacaggactttcggctgctcggccaaacatctgttgaccgtttgctacaactgtcccagggtcaggcagtgaagggcaaccaactccttcctgttagtttggtcaaacgtaaaaccaccctcgcccctaacacccaaactgcctcaccgcgcgcgctagccgatagtctgatgcagctggcacgtcaggtaagtcgtttggaaagcggtcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z