LOCUS       BBa_M1636               2214 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive promoter emits green bioluminescence for positive 
            control
ACCESSION   BBa_M1636
VERSION     BBa_M1636.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..2077
                     /label=BBa_M1635
     stem_loop       2097..2140
                     /label=stem_loop
     misc_feature    2086..2165
                     /label=BBa_B0010
     terminator      2086..2165
                     /label=BBa_B0010
     stem_loop       2181..2200
                     /label=T7 TE
     misc_feature    2207..2207
                     /label=stop
     polyA_site      2201..2214
                     /label=polya
     misc_feature    2174..2214
                     /label=BBa_B0012
     terminator      2174..2214
                     /label=BBa_B0012
     terminator      2086..2214
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatggtcgac ctgtgtgtct tccgtaaact gatattgcag agccgcgaaa aaaaaatgtt
      121 atttacggcg ccgaatccga atacattggg taaactctat tgctggcgca acgctctgca
      181 atgtccctcg gcgttcccgc atacggccag taacatcagg tgtgatggca tcgcggtcat
      241 ctcccgaatt ttccgtcatt actgtcaggc ccgaacgaaa agcagcaagc tgtggattca
      301 ggatgaatgc tcagttgacc tttgtgagca gaaaattttt catccccatt acttctctct
      361 ggtatactgg tatggttgtt caacatgtcg tttgcatcct cgtacgctgt cccacggcta
      421 tattaaaacc aatcttgtct tcactaccag gaagatatta cctaaggtac tggaagtaaa
      481 agatcggacc aactacatca aaaggatcat aattcttgac agtgaggaaa acctgttagg
      541 ctgtgaatct ctgcataatt ttatgagccg ttattcggat aataatctgc aaacgtttaa
      601 acctcttcat tatgatcccg ttgatcaggt ggcagccatc ctatgtagct caggaaccac
      661 ggggttgcca aagggtgtga tgcagactca tcgtaacata tgcgtgcgtc tcacacacgc
      721 gagtgaccct agagtcggca cacagctgat cccgggtgtg tccgttctgg catatcttcc
      781 atttttccat gcatttggct tttccataaa tctggggtat ttcatggttg gcctgcgggt
      841 cgttatgttg cgcaggttta atcaggaagt tttcctgaaa gcaatccagg attatgaggt
      901 ccgcagtgtg ataaacgttc cgtcaaccat tctgtttctg tccaagtctc ctttggtcga
      961 taaatatgac ttgctgtggc gcaactgcgt cgctgctctt catcaccaga ggaaactgtt
     1021 acgacagaat gacacatgcc aggaatttgt ggttgacatg gttcaatcct tactgcaatt
     1081 aatctacatt ttatttacca tgaacctcag ccaagatcat ttagaaaagt ctttactgat
     1141 ctggctgagg aagattggca cgctggtgaa actgtgggat cagatcaaat tagaaaatta
     1201 cgcgagcggc gtgctctggc aaaaagttac aaccatccac aaacctccta aacgtttgct
     1261 tatgactacg gtaggattca ctcttgagac actagatacc atgatgcgga tgaatatcag
     1321 tatttggaca gtgacccgga acctcctgaa cattaaggct ataagacact tgaataaacg
     1381 cttttattat aacattcagg ttgaaatgct tttatccctg gtgtttctga tcaaactcga
     1441 gaactatcaa ctggggagct ggctgaacaa tcccgaacat aatcttcaac agaaaaaatt
     1501 caccattttt ctgccgaacg ggtcgttaat tcaatccatc tgtgtggaag aatttgattc
     1561 tctcatacaa tatccgggca tgctccaggt taagttccag gagaaaaact tcgaaagacg
     1621 gtggaaaaaa ctgctgaact tcaaaagcct cgcctggatt aaaaaagaat gggatatttt
     1681 tactaacaat atttggatta acgcgcctat taaaaaaaag aagaaattgg tcgtagtggt
     1741 ggcagcgctg cactttccgg ttgtttcagc cgctgtggtt gtgttggtcg agccgatgga
     1801 gcgagccagc tttacgagca cgttctgttg gcgaaaagga actcggaatt ggtggtgccg
     1861 cacgtgctgt tctattcgga agtgtaagtg gacatggggg agtcaactgt ttttcctgtc
     1921 tcgcaaacgt acatctaccc tccagtactc gtgctctcag atcctaaact gcaataagta
     1981 taatgcgcag aactcttact ttattaattt gcagtgtttc ttatattctt ttatgctcgt
     2041 tttaatcacc atattcttta atttgatctt ttaataatac tagagccagg catcaaataa
     2101 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2161 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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