BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_M1635 1 BBa_M1635 Pyrearinus termitilluminans prothoracic luciferase 2013-04-23T11:00:00Z 2015-05-08T01:13:57Z AF116843 pH insensitive luciferase E.coli optimized cut sites taken out for standardized part can be used to report similar to GFP, but it glows and doesn't fluoresce false false _768_ 0 16143 9 Not in stock false pH insensitive luciferase E.coli optimized cut sites taken out for standardized part false Chauncey Tucker BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M1636 1 BBa_M1636 Constitutive promoter emits green bioluminescence for positive control 2013-04-23T11:00:00Z 2015-05-08T01:13:57Z Composite Promoter binds hydrogen gas and emits green bioluminescence independent of pH Promoter only turns on with Hydrogen gas false false _768_ 0 16143 9 Not in stock false Make sure the hydrogen binds and turns promoter on false Chauncey Tucker component2216728 1 BBa_J23119 component2216738 1 BBa_B0015 component2216730 1 BBa_B0034 component2216731 1 BBa_M1635 annotation2216728 1 BBa_J23119 range2216728 1 1 35 annotation2216730 1 BBa_B0034 range2216730 1 44 55 annotation2216731 1 BBa_M1635 range2216731 1 62 2077 annotation2216738 1 BBa_B0015 range2216738 1 2086 2214 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_M1636_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M1635_sequence 1 atggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z