BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K233004 1 BBa_K233004 lac operator 2009-07-09T11:00:00Z 2015-05-08T01:11:35Z E.coli This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI false false _329_ 0 4258 9 It's complicated true had it synthesized false Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_M1631 1 BBa_M1631 Mutated lacI 1 2013-04-11T11:00:00Z 2015-05-08T01:13:57Z LacI amino acid sequence with H2 binding-related amino acids, as discussed in www.sciencemag.org/cgi/content/321/5888/572/DC1 (GenBank: AAB98710.1) lacI was mutated by removing the lactose binding site (AA 75,76,79)and replaced by hydrogen binding site (His, His, Cys) false false _768_ 0 16147 9 Not in stock false Back translated and optimized to remove standard restriction sites false Oumou Diallo annotation2214850 1 Codes for H2 binding site range2214850 1 223 237 BBa_M1637 1 BBa_M1637 LacSwap & Bioluminescence 2013-04-24T11:00:00Z 2015-05-08T01:13:57Z J23119 + B0034+ M1631+ K233004 + M1635 + stop Oumou's LacSwap plus the Lac machinery, plus Chauncey's bioluminescence reporter Promoter + RBS + LacSwap + LacOperator + Chauncey's Biolumin false false _768_ 0 16147 9 Not in stock false Thank you Ryan for your advice on promoters and RBS false Oumou Diallo component2216956 1 BBa_J23119 component2216958 1 BBa_B0034 component2216965 1 BBa_B0012 component2216960 1 BBa_M1631 component2216961 1 BBa_K233004 component2216963 1 BBa_B0010 component2216962 1 BBa_M1635 annotation2216961 1 BBa_K233004 range2216961 1 1150 1179 annotation2216960 1 BBa_M1631 range2216960 1 62 1141 annotation2216956 1 BBa_J23119 range2216956 1 1 35 annotation2216965 1 BBa_B0012 range2216965 1 3298 3338 annotation2216962 1 BBa_M1635 range2216962 1 1186 3201 annotation2216958 1 BBa_B0034 range2216958 1 44 55 annotation2216963 1 BBa_B0010 range2216963 1 3210 3289 BBa_M1635 1 BBa_M1635 Pyrearinus termitilluminans prothoracic luciferase 2013-04-23T11:00:00Z 2015-05-08T01:13:57Z AF116843 pH insensitive luciferase E.coli optimized cut sites taken out for standardized part can be used to report similar to GFP, but it glows and doesn't fluoresce false false _768_ 0 16143 9 Not in stock false pH insensitive luciferase E.coli optimized cut sites taken out for standardized part false Chauncey Tucker BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K233004_sequence 1 tgtggaattgtgagcgctcacaattccaca BBa_B0034_sequence 1 aaagaggagaaa BBa_M1631_sequence 1 atgaaacctgtgactctgtatgatgttgcggagtatgcaggtgtctcgtaccagaccgtgagccgcgttgttaaccaagctagtcatgtcagcgcgaaaacgcgtgagaaagtggaagctgcaatggcggaattgaactacattccaaatcgtgtcgcacagcaactagcgggcaaacaatctcttctgataggcgtagctacgagttctctggcattgcaccatcactcccaatgtgttgcagcgataaagtcacgtgcagatcaattgggtgcgtcggtggtcgtgtctatggtggaaagatcaggtgtggaagcatgcaaagcagcggttcataatctgctggcacaacgtgtatcaggactactgattaactatccccttgacgatcaagatgcgacggccgttgaagccgcatgcgctaatgtccctgcccttttcttagacgttagtgatcaaactccgatcaattcaatcatttttagccatgaggatggcactcggcttggcgtagagcacttagtagcgttgggacatcaacagattgctcttttggcgggcccattaagcagtgtgtcagcgcgtttgcgactagccggctggcacaaatatttgacccgcaaccaaatccagccgattgctgagcgggagggcgattggtcggcaatgtctggctttcagcaaacgatgcaaatgctgaatgaaggaatcgtcccaactgcaatgctggtggctaacgatcagatggccttaggcgcgatgcgtgcaatcaccgagtctggactgcgggtgggagcagatatatcggtcgttgggtatgatgatacagaagactcttcatgttacattccgccgctgactacgatcaaacaggactttcggctgctcggccaaacatctgttgaccgtttgctacaactgtcccagggtcaggcagtgaagggcaaccaactccttcctgttagtttggtcaaacgtaaaaccaccctcgcccctaacacccaaactgcctcaccgcgcgcgctagccgatagtctgatgcagctggcacgtcaggtaagtcgtttggaaagcggtcag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_M1637_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaaacctgtgactctgtatgatgttgcggagtatgcaggtgtctcgtaccagaccgtgagccgcgttgttaaccaagctagtcatgtcagcgcgaaaacgcgtgagaaagtggaagctgcaatggcggaattgaactacattccaaatcgtgtcgcacagcaactagcgggcaaacaatctcttctgataggcgtagctacgagttctctggcattgcaccatcactcccaatgtgttgcagcgataaagtcacgtgcagatcaattgggtgcgtcggtggtcgtgtctatggtggaaagatcaggtgtggaagcatgcaaagcagcggttcataatctgctggcacaacgtgtatcaggactactgattaactatccccttgacgatcaagatgcgacggccgttgaagccgcatgcgctaatgtccctgcccttttcttagacgttagtgatcaaactccgatcaattcaatcatttttagccatgaggatggcactcggcttggcgtagagcacttagtagcgttgggacatcaacagattgctcttttggcgggcccattaagcagtgtgtcagcgcgtttgcgactagccggctggcacaaatatttgacccgcaaccaaatccagccgattgctgagcgggagggcgattggtcggcaatgtctggctttcagcaaacgatgcaaatgctgaatgaaggaatcgtcccaactgcaatgctggtggctaacgatcagatggccttaggcgcgatgcgtgcaatcaccgagtctggactgcgggtgggagcagatatatcggtcgttgggtatgatgatacagaagactcttcatgttacattccgccgctgactacgatcaaacaggactttcggctgctcggccaaacatctgttgaccgtttgctacaactgtcccagggtcaggcagtgaagggcaaccaactccttcctgttagtttggtcaaacgtaaaaccaccctcgcccctaacacccaaactgcctcaccgcgcgcgctagccgatagtctgatgcagctggcacgtcaggtaagtcgtttggaaagcggtcagtactagagtgtggaattgtgagcgctcacaattccacatactagatggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M1635_sequence 1 atggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z