LOCUS BBa_M1641 3906 bp DNA linear 23-Dec-2024 DEFINITION Complete proteorhodopsin and hydrogenase device, producing hydrogen gas. Constitutive promoter. ACCESSION BBa_M1641 VERSION BBa_M1641.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..64 /label=Start misc_feature 816..819 /label=Stop CDS 62..819 /label=BBa_K572005 misc_feature 832..835 /label=conserved RBS 828..839 /label=BBa_B0034 CDS 846..3769 /label=BBa_M1633 stem_loop 3789..3832 /label=stem_loop misc_feature 3778..3857 /label=BBa_B0010 terminator 3778..3857 /label=BBa_B0010 stem_loop 3873..3892 /label=T7 TE misc_feature 3899..3899 /label=stop polyA_site 3893..3906 /label=polya misc_feature 3866..3906 /label=BBa_B0012 terminator 3866..3906 /label=BBa_B0012 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgaaatta ttactgatat taggtagtgt tattgcactt cctacatttg ccgcaggtgg 121 tggtgacctt gatgctagtg attacactgg tgtttctttt tggttagtta ctgctgcttt 181 attagcatct actgtatttt tctttgttga aagagataga gtttctgcaa aatggaaaac 241 atcattaact gtatctggtc ttgttactgg tattgctttc tggcattaca tgtacatgag 301 aggggtatgg attgaaactg gtgattcgcc aactgtattt agatacattg attggttact 361 aacagttcct ctattaatat gtgagttcta cttaattctt gctgctgcaa ctaatgttgc 421 tggatcatta tttaagaaac tgctagttgg ttctcttgtt atgcttgtgt ttggttacat 481 gggtgaagca ggaatcatgg ctgcatggcc tgcattcatt attgggtgtt tagcttgggt 541 atacatgatt tatgaattat gggctggaga aggaaaatct gcatgtaata ctgcaagtcc 601 tgctgtgcaa tcagcttaca acacaatgat gtatattatc atctttggtt gggcgattta 661 tcctgtaggt tatttcacag gttacctgat gggtgacggt ggatcagctc ttaacttaaa 721 ccttatctat aaccttgctg actttgttaa caagattcta tttggtttaa ttatatggaa 781 tgttgctgtt aaagaatctt ctaatgctta actaataact actagagaaa gaggagaaat 841 actagatgtc atctcaagtt gaaacgttct atgaagtcat gcggcgccaa ggaattacgc 901 gtcgcagctt tttgaaatac tgtagcctta ctgcggctgc attagggctt agtcctgctt 961 atgcgaacaa aattgctcat gcgatggaaa caaagcctcg cacgcctgtt atctggttgc 1021 atggtttgga atgtacgtgt tgttcagaat cctttattcg ttctgctcac ccactagcaa 1081 aagacgtagt gttatccatg atctctctgg attatgacga caccctcatg gcagcctctg 1141 gacatgcagc ggaagcgatt cttgatgaaa ttaaagaaaa atataaagga aactatattc 1201 ttgcggtaga agggaaccct ccgcttaatc aggatggaat gtcctgcatt atcggtggcc 1261 gccctttttc cgaacaatta aaacgcatgg ccgatgatgc aaaagctatc atttcatggg 1321 gttcttgcgc gtcatgggga tgcgtacagg cagcaaaacc aaaccctacg caggccacgc 1381 cagtacataa atttcttggc ggcgggtatg acaaaccgat tatcaaagtg cctgggtgtc 1441 ctccaattgc tgaagtcatg actggcgtca ttacctatat gctaaccttt gatcgcatcc 1501 ctgagctgga ccgacaaggt cgtcctaaaa tgttctactc acaacgtatt catgataaat 1561 gctaccgccg ccctcacttt gatgccggtc aatttgtaga agagtgggat gacgaaggtg 1621 ctcgtaaagg ttactgttta tacaaagttg gatgcaaagg cccaacaact tataacgcct 1681 gctcaaccgt ccgttggaat ggaggaactt ccttcccgat tcaatctggt catggctgta 1741 tcggctgttc tgaagatggg ttctgggaca aaggatcctt ctactcacgc gatacagaga 1801 tgaacgcatt tggcattgaa gcaacggcag acgatattgg taaaaccgcc atcggtgttg 1861 tcggtgcagc agtagtcgct catgcagcga taagtgctgt aaaagcggct caaaagaaag 1921 gagataaata ataatgagcg tattaaacac accaaaccac tataagatgg acaactccgg 1981 tcgtcgagta gtcattgatc ctgttactcg tatcgaaggc cacatgcgtt gtgaagtcaa 2041 cgttgatgaa aacaatgtca tccaaaatgc cgtatccaca ggcactatgt ggcgaggcct 2101 tgaggttatt cttcgcggac gcgaccctcg tgacgcatgg gcatttgtag aacgcatatg 2161 tggtgtctgc actggctgcc atgctttggc atcagttcga gcggttgagg atgctttaga 2221 tatcaaaatt ccgcacaatg ccactttaat tcgcgaaatt atggccaaaa cacttcaaat 2281 tcatgaccat attgtgcatt tctaccattt acatgctctt gactgggtca atccagtaaa 2341 tgctttgaag gcagatcctc aggcaacctc tgaactacaa aaacttgtct caccgcatca 2401 cccaatgtct agccctggct acttcaaaga cattcaaatc agaatccaaa agttcgtaga 2461 ttctgggcaa ctcggaatct tcaagaatgg ttattggagt aaccccgcct ataaattatc 2521 tcccgaagca gatcttatgg ctgttaccca ctatcttgaa gctctggact tccaaaaaga 2581 aatcgtcaaa atccatgcga tattcggtgg taaaaaccct caccctaact atatggttgg 2641 aggtgtgcct tgtgcaataa acatcgatgg agatatggcc gcgggtgccc ctataaacat 2701 ggagcgttta aactttgtca aatcacttat agaacaaggg cgaaccttta acaccaatgt 2761 ctatgtaccc gacgttatag ctatcgcagc tttctatcgc gactggctat atggtggagg 2821 gctatccgcg acaaacgtta tggattatgg cgcataccct aaaactccat atgataaatc 2881 tacagatcaa ctacctggag gtgcaatcat taatggagat tgggggaaaa ttcatccagt 2941 tgaccctaga gatcctgagc aggttcaaga atttgtcact cattcttggt acaaataccc 3001 agacgaaaca aaaggacttc atccatggga tggtattaca gagccaaact atgaactagg 3061 atctaaaaca aaagggtcga gaacaaacat catcgaaatc gacgaatctg caaaatattc 3121 ctggatcaaa tccccgcgct ggagggggca tgctgtcgaa gtagggccac ttgcacgcta 3181 tatacttgcc tatgcccaag gcgttgaata cgttaagact caggttcaca caagcctaaa 3241 taggtttaac gctgtatgtc gtttgctaga cccaaaccat aaagacatca ccgacctgaa 3301 agctttcctt ggatcaacaa tcggccgaac tcttgctcgt gctcttgaat cagagtattg 3361 cggagacatg atgctagatg acttcaacca attgatcagc aacattaaaa acggtgatag 3421 cagtacggct aatacagaca agtgggatcc ttcgagctgg cctgaacatg ccaaaggtgt 3481 gggaacagtt gcagctcctc gcggtgctct ggcgcactgg attgttattg agaaaggaaa 3541 gatcaaaaac taccagtgtg ttgtgccaac aacttggaat ggctctcctc gtgaccctaa 3601 aggaaatata ggagcttttg aagcatctct tatgggaact ccaatggaac gcccagatga 3661 accggtcgaa gtacttcgta cccttcacag ttttgatccg tgcttagcgt gttcaacgca 3721 tgttatgagt gaagaaggcg aagaaatggc gaccgtcaag gttcgataat actagagcca 3781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3901 tttata //