BBa_K572005 1 BBa_K572005 Proteorhodopsin 2011-09-17T11:00:00Z 2015-05-08T01:12:44Z Source Organism : Marine gamma proteobacterium EBAC31A08 We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene. Proteorhodopsin, a photon dependent proton pump, is a photoactive retinylidene protein. A transmembrane protein homologous to bacteriorhodopsin in Archaea creates a proton gradient across the cell membrane in many marine organisms. It has been demonstrated that photophosphorylation occurs with the help of transmembrane ATP Synthase on expression of Proteorhodopsin gene in Escherichia coli* *Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590???5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221. false false _743_ 0 6058 9 It's complicated false The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone. false Avaneesh T annotation2155682 1 Start range2155682 1 1 3 annotation2155683 1 Stop range2155683 1 755 758 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_M1633 1 BBa_M1633 Hydrogenase from Hydrogenovibrio marinus 2013-04-19T11:00:00Z 2015-05-08T01:13:57Z GenBank: AB070719.1 Hydrogenase that convert proton to hydrogen gas false false _768_ 0 16141 9 Not in stock false Nothing false Bo Zhao BBa_M1641 1 BBa_M1641 Complete proteorhodopsin and hydrogenase device, producing hydrogen gas. Constitutive promoter. 2013-04-25T11:00:00Z 2015-05-08T01:13:57Z Proteorhodopsin, used from registry. Hydrogenase isolated from Hydrogenovibrio marinus. Device produces biohydrogen as part of a two cell system. false false _768_ 0 13965 9 Not in stock false false Ryan Putman component2216925 1 BBa_B0034 component2216934 1 BBa_B0012 component2216923 1 BBa_J23119 component2216930 1 BBa_B0034 component2216932 1 BBa_B0010 component2216931 1 BBa_M1633 component2216928 1 BBa_K572005 annotation2216931 1 BBa_M1633 range2216931 1 846 3769 annotation2216932 1 BBa_B0010 range2216932 1 3778 3857 annotation2216925 1 BBa_B0034 range2216925 1 44 55 annotation2216934 1 BBa_B0012 range2216934 1 3866 3906 annotation2216928 1 BBa_K572005 range2216928 1 62 819 annotation2216930 1 BBa_B0034 range2216930 1 828 839 annotation2216923 1 BBa_J23119 range2216923 1 1 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_M1633_sequence 1 atgtcatctcaagttgaaacgttctatgaagtcatgcggcgccaaggaattacgcgtcgcagctttttgaaatactgtagccttactgcggctgcattagggcttagtcctgcttatgcgaacaaaattgctcatgcgatggaaacaaagcctcgcacgcctgttatctggttgcatggtttggaatgtacgtgttgttcagaatcctttattcgttctgctcacccactagcaaaagacgtagtgttatccatgatctctctggattatgacgacaccctcatggcagcctctggacatgcagcggaagcgattcttgatgaaattaaagaaaaatataaaggaaactatattcttgcggtagaagggaaccctccgcttaatcaggatggaatgtcctgcattatcggtggccgccctttttccgaacaattaaaacgcatggccgatgatgcaaaagctatcatttcatggggttcttgcgcgtcatggggatgcgtacaggcagcaaaaccaaaccctacgcaggccacgccagtacataaatttcttggcggcgggtatgacaaaccgattatcaaagtgcctgggtgtcctccaattgctgaagtcatgactggcgtcattacctatatgctaacctttgatcgcatccctgagctggaccgacaaggtcgtcctaaaatgttctactcacaacgtattcatgataaatgctaccgccgccctcactttgatgccggtcaatttgtagaagagtgggatgacgaaggtgctcgtaaaggttactgtttatacaaagttggatgcaaaggcccaacaacttataacgcctgctcaaccgtccgttggaatggaggaacttccttcccgattcaatctggtcatggctgtatcggctgttctgaagatgggttctgggacaaaggatccttctactcacgcgatacagagatgaacgcatttggcattgaagcaacggcagacgatattggtaaaaccgccatcggtgttgtcggtgcagcagtagtcgctcatgcagcgataagtgctgtaaaagcggctcaaaagaaaggagataaataataatgagcgtattaaacacaccaaaccactataagatggacaactccggtcgtcgagtagtcattgatcctgttactcgtatcgaaggccacatgcgttgtgaagtcaacgttgatgaaaacaatgtcatccaaaatgccgtatccacaggcactatgtggcgaggccttgaggttattcttcgcggacgcgaccctcgtgacgcatgggcatttgtagaacgcatatgtggtgtctgcactggctgccatgctttggcatcagttcgagcggttgaggatgctttagatatcaaaattccgcacaatgccactttaattcgcgaaattatggccaaaacacttcaaattcatgaccatattgtgcatttctaccatttacatgctcttgactgggtcaatccagtaaatgctttgaaggcagatcctcaggcaacctctgaactacaaaaacttgtctcaccgcatcacccaatgtctagccctggctacttcaaagacattcaaatcagaatccaaaagttcgtagattctgggcaactcggaatcttcaagaatggttattggagtaaccccgcctataaattatctcccgaagcagatcttatggctgttacccactatcttgaagctctggacttccaaaaagaaatcgtcaaaatccatgcgatattcggtggtaaaaaccctcaccctaactatatggttggaggtgtgccttgtgcaataaacatcgatggagatatggccgcgggtgcccctataaacatggagcgtttaaactttgtcaaatcacttatagaacaagggcgaacctttaacaccaatgtctatgtacccgacgttatagctatcgcagctttctatcgcgactggctatatggtggagggctatccgcgacaaacgttatggattatggcgcataccctaaaactccatatgataaatctacagatcaactacctggaggtgcaatcattaatggagattgggggaaaattcatccagttgaccctagagatcctgagcaggttcaagaatttgtcactcattcttggtacaaatacccagacgaaacaaaaggacttcatccatgggatggtattacagagccaaactatgaactaggatctaaaacaaaagggtcgagaacaaacatcatcgaaatcgacgaatctgcaaaatattcctggatcaaatccccgcgctggagggggcatgctgtcgaagtagggccacttgcacgctatatacttgcctatgcccaaggcgttgaatacgttaagactcaggttcacacaagcctaaataggtttaacgctgtatgtcgtttgctagacccaaaccataaagacatcaccgacctgaaagctttccttggatcaacaatcggccgaactcttgctcgtgctcttgaatcagagtattgcggagacatgatgctagatgacttcaaccaattgatcagcaacattaaaaacggtgatagcagtacggctaatacagacaagtgggatccttcgagctggcctgaacatgccaaaggtgtgggaacagttgcagctcctcgcggtgctctggcgcactggattgttattgagaaaggaaagatcaaaaactaccagtgtgttgtgccaacaacttggaatggctctcctcgtgaccctaaaggaaatataggagcttttgaagcatctcttatgggaactccaatggaacgcccagatgaaccggtcgaagtacttcgtacccttcacagttttgatccgtgcttagcgtgttcaacgcatgttatgagtgaagaaggcgaagaaatggcgaccgtcaaggttcgataa BBa_M1641_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataactactagagaaagaggagaaatactagatgtcatctcaagttgaaacgttctatgaagtcatgcggcgccaaggaattacgcgtcgcagctttttgaaatactgtagccttactgcggctgcattagggcttagtcctgcttatgcgaacaaaattgctcatgcgatggaaacaaagcctcgcacgcctgttatctggttgcatggtttggaatgtacgtgttgttcagaatcctttattcgttctgctcacccactagcaaaagacgtagtgttatccatgatctctctggattatgacgacaccctcatggcagcctctggacatgcagcggaagcgattcttgatgaaattaaagaaaaatataaaggaaactatattcttgcggtagaagggaaccctccgcttaatcaggatggaatgtcctgcattatcggtggccgccctttttccgaacaattaaaacgcatggccgatgatgcaaaagctatcatttcatggggttcttgcgcgtcatggggatgcgtacaggcagcaaaaccaaaccctacgcaggccacgccagtacataaatttcttggcggcgggtatgacaaaccgattatcaaagtgcctgggtgtcctccaattgctgaagtcatgactggcgtcattacctatatgctaacctttgatcgcatccctgagctggaccgacaaggtcgtcctaaaatgttctactcacaacgtattcatgataaatgctaccgccgccctcactttgatgccggtcaatttgtagaagagtgggatgacgaaggtgctcgtaaaggttactgtttatacaaagttggatgcaaaggcccaacaacttataacgcctgctcaaccgtccgttggaatggaggaacttccttcccgattcaatctggtcatggctgtatcggctgttctgaagatgggttctgggacaaaggatccttctactcacgcgatacagagatgaacgcatttggcattgaagcaacggcagacgatattggtaaaaccgccatcggtgttgtcggtgcagcagtagtcgctcatgcagcgataagtgctgtaaaagcggctcaaaagaaaggagataaataataatgagcgtattaaacacaccaaaccactataagatggacaactccggtcgtcgagtagtcattgatcctgttactcgtatcgaaggccacatgcgttgtgaagtcaacgttgatgaaaacaatgtcatccaaaatgccgtatccacaggcactatgtggcgaggccttgaggttattcttcgcggacgcgaccctcgtgacgcatgggcatttgtagaacgcatatgtggtgtctgcactggctgccatgctttggcatcagttcgagcggttgaggatgctttagatatcaaaattccgcacaatgccactttaattcgcgaaattatggccaaaacacttcaaattcatgaccatattgtgcatttctaccatttacatgctcttgactgggtcaatccagtaaatgctttgaaggcagatcctcaggcaacctctgaactacaaaaacttgtctcaccgcatcacccaatgtctagccctggctacttcaaagacattcaaatcagaatccaaaagttcgtagattctgggcaactcggaatcttcaagaatggttattggagtaaccccgcctataaattatctcccgaagcagatcttatggctgttacccactatcttgaagctctggacttccaaaaagaaatcgtcaaaatccatgcgatattcggtggtaaaaaccctcaccctaactatatggttggaggtgtgccttgtgcaataaacatcgatggagatatggccgcgggtgcccctataaacatggagcgtttaaactttgtcaaatcacttatagaacaagggcgaacctttaacaccaatgtctatgtacccgacgttatagctatcgcagctttctatcgcgactggctatatggtggagggctatccgcgacaaacgttatggattatggcgcataccctaaaactccatatgataaatctacagatcaactacctggaggtgcaatcattaatggagattgggggaaaattcatccagttgaccctagagatcctgagcaggttcaagaatttgtcactcattcttggtacaaatacccagacgaaacaaaaggacttcatccatgggatggtattacagagccaaactatgaactaggatctaaaacaaaagggtcgagaacaaacatcatcgaaatcgacgaatctgcaaaatattcctggatcaaatccccgcgctggagggggcatgctgtcgaagtagggccacttgcacgctatatacttgcctatgcccaaggcgttgaatacgttaagactcaggttcacacaagcctaaataggtttaacgctgtatgtcgtttgctagacccaaaccataaagacatcaccgacctgaaagctttccttggatcaacaatcggccgaactcttgctcgtgctcttgaatcagagtattgcggagacatgatgctagatgacttcaaccaattgatcagcaacattaaaaacggtgatagcagtacggctaatacagacaagtgggatccttcgagctggcctgaacatgccaaaggtgtgggaacagttgcagctcctcgcggtgctctggcgcactggattgttattgagaaaggaaagatcaaaaactaccagtgtgttgtgccaacaacttggaatggctctcctcgtgaccctaaaggaaatataggagcttttgaagcatctcttatgggaactccaatggaacgcccagatgaaccggtcgaagtacttcgtacccttcacagttttgatccgtgcttagcgtgttcaacgcatgttatgagtgaagaaggcgaagaaatggcgaccgtcaaggttcgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K572005_sequence 1 atgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z