LOCUS       BBa_M1642               2042 bp    DNA     linear       22-Dec-2024
DEFINITION  LacSwap + repressor binding site + GFP reporter
ACCESSION   BBa_M1642
VERSION     BBa_M1642.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    284..298
                     /label=Codes for H2 binding site
     promoter        62..1141
                     /label=BBa_M1631
     promoter        1150..1179
                     /label=BBa_K233004
     CDS             1186..1905
                     /label=GFP protein
     CDS             1186..1905
                     /label=BBa_E0040
     stem_loop       1925..1968
                     /label=stem_loop
     misc_feature    1914..1993
                     /label=BBa_B0010
     terminator      1914..1993
                     /label=BBa_B0010
     stem_loop       2009..2028
                     /label=T7 TE
     misc_feature    2035..2035
                     /label=stop
     polyA_site      2029..2042
                     /label=polya
     misc_feature    2002..2042
                     /label=BBa_B0012
     terminator      2002..2042
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaacct gtgactctgt atgatgttgc ggagtatgca ggtgtctcgt accagaccgt
      121 gagccgcgtt gttaaccaag ctagtcatgt cagcgcgaaa acgcgtgaga aagtggaagc
      181 tgcaatggcg gaattgaact acattccaaa tcgtgtcgca cagcaactag cgggcaaaca
      241 atctcttctg ataggcgtag ctacgagttc tctggcattg caccatcact cccaatgtgt
      301 tgcagcgata aagtcacgtg cagatcaatt gggtgcgtcg gtggtcgtgt ctatggtgga
      361 aagatcaggt gtggaagcat gcaaagcagc ggttcataat ctgctggcac aacgtgtatc
      421 aggactactg attaactatc cccttgacga tcaagatgcg acggccgttg aagccgcatg
      481 cgctaatgtc cctgcccttt tcttagacgt tagtgatcaa actccgatca attcaatcat
      541 ttttagccat gaggatggca ctcggcttgg cgtagagcac ttagtagcgt tgggacatca
      601 acagattgct cttttggcgg gcccattaag cagtgtgtca gcgcgtttgc gactagccgg
      661 ctggcacaaa tatttgaccc gcaaccaaat ccagccgatt gctgagcggg agggcgattg
      721 gtcggcaatg tctggctttc agcaaacgat gcaaatgctg aatgaaggaa tcgtcccaac
      781 tgcaatgctg gtggctaacg atcagatggc cttaggcgcg atgcgtgcaa tcaccgagtc
      841 tggactgcgg gtgggagcag atatatcggt cgttgggtat gatgatacag aagactcttc
      901 atgttacatt ccgccgctga ctacgatcaa acaggacttt cggctgctcg gccaaacatc
      961 tgttgaccgt ttgctacaac tgtcccaggg tcaggcagtg aagggcaacc aactccttcc
     1021 tgttagtttg gtcaaacgta aaaccaccct cgcccctaac acccaaactg cctcaccgcg
     1081 cgcgctagcc gatagtctga tgcagctggc acgtcaggta agtcgtttgg aaagcggtca
     1141 gtactagagt gtggaattgt gagcgctcac aattccacat actagatgcg taaaggagaa
     1201 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1261 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1321 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1381 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1441 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1501 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1561 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     1621 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     1681 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1741 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1801 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1861 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca
     1921 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1981 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2041 ta
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