LOCUS BBa_M1656 3027 bp DNA linear 15-Nov-2024 DEFINITION Group3 ACCESSION BBa_M1656 VERSION BBa_M1656.1 FEATURES Location/Qualifiers promoter 2498..2525 /label=putative E. coli promoter CDS 1..2655 /label=BBa_I2032 RBS 2664..2675 /label=BBa_J61100 misc_feature 2682..2684 /label=start misc_feature 2979..2981 /label=stop CDS 2685..2978 /label=CXC CDS 2682..2978 /label=BBa_M1652 stem_loop 2994..3013 /label=T7 TE misc_feature 3020..3020 /label=stop polyA_site 3014..3027 /label=polya misc_feature 2987..3027 /label=BBa_B0012 terminator 2987..3027 /label=BBa_B0012 ORIGIN 1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg 61 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag 121 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa 181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag 241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg 301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag 361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca 421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag 481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa 541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg 601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc 661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac 721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg 781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc 841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac 901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt 961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta 1021 atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc 1081 ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct 1141 gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc 1201 atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg 1261 gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc 1321 aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg 1381 aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag 1441 ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact 1501 tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg 1561 gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc 1621 tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac 1681 ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag 1741 attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag 1801 aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg 1861 ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg 1921 tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat 1981 tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg 2041 atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag 2101 tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc 2161 aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag 2221 aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc 2281 attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct 2341 aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag 2401 aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac 2461 gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat 2521 gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa 2581 atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc 2641 gcgttcgcgt aataatacta gagaaagagg ggacatacta gatgcgtctg ctggctgcgg 2701 cactgttact gctgctgttg gcgttgtata ccgctcgcgt tgatggcagt aaatgtaaat 2761 gttcacgcaa aggtccgaaa attcgctact ctgatgtgaa gaaactggaa atgaaaccga 2821 aatatccgca ctgtgaagaa aaaatggtta tcatcaccac gaaaagcgtt agccggtatc 2881 gcgggcagga acactgcttg catccgaaac tccagtccac caaacgcttt atcaagtggt 2941 ataatgcttg gaatgaaaaa agacgcgtgt acgaggagta ctagagtcac actggctcac 3001 cttcgggtgg gcctttctgc gtttata //