LOCUS BBa_M1657 9333 bp DNA linear 15-Nov-2024 DEFINITION Group3 1 ACCESSION BBa_M1657 VERSION BBa_M1657.1 FEATURES Location/Qualifiers RBS 2664..2675 /label=BBa_J61100 misc_feature 2682..2684 /label=start misc_feature 9285..9287 /label=stop CDS 2685..9284 /label=Tenascin-C CDS 2682..9284 /label=BBa_M1653 stem_loop 9300..9319 /label=T7 TE misc_feature 9326..9326 /label=stop polyA_site 9320..9333 /label=polya misc_feature 9293..9333 /label=BBa_B0012 terminator 9293..9333 /label=BBa_B0012 ORIGIN 1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg 61 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag 121 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa 181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag 241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg 301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag 361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca 421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag 481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa 541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg 601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc 661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac 721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg 781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc 841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac 901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt 961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta 1021 atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc 1081 ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct 1141 gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc 1201 atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg 1261 gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc 1321 aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg 1381 aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag 1441 ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact 1501 tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg 1561 gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc 1621 tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac 1681 ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag 1741 attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag 1801 aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg 1861 ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg 1921 tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat 1981 tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg 2041 atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag 2101 tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc 2161 aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag 2221 aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc 2281 attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct 2341 aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag 2401 aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac 2461 gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat 2521 gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa 2581 atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc 2641 gcgttcgcgt aataatacta gagaaagagg ggacatacta gatgggggcc atgacccaat 2701 tactggccgg tgttttcctg gcgtttctgg ctctggcgac agaaggtggt gtactgaaaa 2761 aagtgattcg ccacaagcgg caaagcggag taaatgcgac gcttccggaa gagaatcagc 2821 cggttgtgtt caaccatgtt tataacatca aactgccggt gggttcccag tgttctgttg 2881 atttggagag tgcgagtgga gaaaaggacc tagcgccgcc ttcagagccg tcggagtctt 2941 tccaggaaca tacagttgat ggtgaaaatc aaattgtgtt tacccaccgt atcaatatcc 3001 cgcgtcgcgc ctgcggttgt gctgccgccc cagacgttaa agagcttctg tcacgacttg 3061 aagaactgga gaacttagtg agcagtctgc gtgaacagtg cacggcgggt gccggctgct 3121 gcctccagcc ggcaacaggt cgcctggaca cccgcccctt ctgctcgggg cgaggcaatt 3181 ttagcacgga aggttgcggc tgcgtatgtg aacctgggtg gaaaggtcct aattgttctg 3241 aaccagaatg ccctgggaat tgtcatctgc gcgggcgctg cattgacggc cagtgcattt 3301 gtgacgatgg ttttactggc gaggactgtt cacaattggc ctgcccatca gactgcaatg 3361 atcaaggtaa gtgcgtgaat ggcgtatgca tttgcttcga aggttatgcg ggcgctgact 3421 gctctcgtga aatctgtcca gtaccctgct ccgaagaaca tggcacttgt gtggacgggt 3481 tatgtgtatg tcacgacgga tttgccggcg atgattgtaa taaacctctt tgtctgaaca 3541 actgttataa tcgcggtcgc tgtgtagaaa atgaatgcgt atgtgatgaa ggatttaccg 3601 gtgaggattg cagtgaactg atttgtccta acgattgctt tgaccgggga cgatgcatca 3661 acggcacctg ctactgtgag gagggcttca ccggtgagga ctgcggtaaa ccgacctgtc 3721 cgcacgcttg tcacacccaa ggtcgctgcg aggagggcca gtgtgtctgt gatgaaggct 3781 tcgccggcgt tgattgctcc gaaaagcgct gcccagcgga ttgccataac cgggggcgtt 3841 gcgttgatgg tcggtgcgag tgcgacgacg gtttcaccgg agcggattgt ggcgaactga 3901 aatgcccgaa cggctgctct ggccacggcc gttgtgtgaa tggccagtgt gtttgcgatg 3961 agggctatac cggcgaagat tgttctcaac tgcgttgccc caatgactgc catagccgtg 4021 ggcgctgtgt cgaaggaaag tgtgtgtgtg agcaaggctt taaaggttat gattgctcag 4081 atatgagctg tcctaatgac tgtcatcagc acgggcgctg tgtaaatggt atgtgcgttt 4141 gtgatgatgg ctacaccgga gaggattgcc gcgatcgtca atgtccgcgt gactgttcta 4201 atcgcggtct gtgtgtggat ggacaatgtg tgtgcgaaga cggcttcacg ggaccggatt 4261 gtgcggaact ctcttgccca aacgactgtc acggtcaagg tcgctgtgtg aacggtcaat 4321 gcgtgtgtca tgaaggtttt atgggcaagg attgtaagga acagcgatgc ccttccgatt 4381 gccacggtca aggacgttgt gtagatggcc agtgcatctg ccatgaagga ttcaccggtc 4441 tcgattgcgg tcagcactcc tgcccgagcg actgtaacaa cctcggccaa tgtgtaagtg 4501 gtcgctgcat ttgcaatgaa ggttactcag gggaagactg tagcgaagtt agcccgccga 4561 aggatttggt ggttacagaa gtcaccgagg aaacggttaa tctggcctgg gacaatgaaa 4621 tgcgggtaac cgaatatctg gtagtttata cacccaccca tgagggcggg ctggaaatgc 4681 agtttcgcgt accgggtgat caaacgagta ccattatcca ggagttagaa ccgggcgtgg 4741 aatatttcat ccgcgtcttc gccattctgg aaaacaaaaa gtccatccct gtatcagctc 4801 gcgttgcaac ctacctgccg gccccggagg gtctcaagtt taagagtatc aaagagacga 4861 gcgttgaagt cgaatgggac ccactcgaca ttgctttcga aacgtgggaa attatctttc 4921 gtaacatgaa taaggaggat gagggcgaaa taaccaaaag cttgcgtcgc ccggagacct 4981 cttatcgcca aacgggattg gcgcctggcc aggagtacga gatttctctt catattgtga 5041 aaaataatac tcgcggtcca ggcctcaagc gtgtgacgac cacgcgcctt gacgcgccct 5101 cacagattga agttaaggat gttactgaca ccacggcgct gattacctgg ttcaaaccgc 5161 ttgcagaaat tgatggcata gaactgacct acggcattaa ggatgtcccg ggtgaccgta 5221 ccaccataga cctgactgag gatgagaacc agtatagtat cggtaatctg aaaccggata 5281 ccgaatatga ggtgagcctg atcagtcgcc gtggagacat gtcgtctaac ccggccaaag 5341 agacctttac cactggcctg gatgcgccac gtaatcttag gcgcgtatcg caaacagaca 5401 actcaattac ccttgaatgg cgtaacggta aagcggccat tgattcgtat cgaattaaat 5461 atgcaccgat ttcaggcggc gaccacgcgg aagttgatgt tcctaaaagc cagcaggcga 5521 ccacgaaaac gaccctgacg ggcctgcgcc cgggaacgga atacggtatt ggcgtatctg 5581 cggtgaaaga agacaaggaa tccaacccgg cgaccattaa cgcggcaaca gaattggata 5641 ctccaaaaga tctacaagta agtgaaactg ctgaaacgag tctcactcta ctgtggaaaa 5701 ctccgctggc caaatttgat cggtatcgtc tcaactacag tctgccaacc ggacagtggg 5761 ttggggtaca gttaccgcgt aacaccacat catatgtttt gcgcggcctg gagccgggcc 5821 aggaatacaa tgtcttgctg acggcggaga aaggccgtca taaaagtaaa ccggcgcgtg 5881 tcaaagcttc gaccgaacag gcaccagaac tggaaaacct gacagttacg gaggttgggt 5941 gggacggttt gcgcttgaac tggaccgccg ccgaccaggc atacgaacat tttatcattc 6001 aggtgcagga agccaacaag gtagaagcag cccgcaatct gaccgtccct gggagcctga 6061 gagctgtgga tattccgggg cttaaagccg cgacaccata tacggtgagt atatacggtg 6121 ttatccaggg ctaccgtacg cctgttctta gcgctgaagc gagcaccggc gaaaccccta 6181 acctgggcga ggtggtggtg gcggaagttg gctgggatgc gctgaaactc aactggacag 6241 cccctgaagg agcctacgaa tatttcttta ttcaggtcca ggaagctgat acagttgagg 6301 cggcacagaa ccttacggtc cccgggggtt tgcgttctac cgatctcccg ggactgaagg 6361 cagcgacgca ctacaccatt acgatcagag gggtcaccca ggattttagt actactcctc 6421 tgagcgtgga agtcctgacc gaagaagttc cggatatggg taatctaaca gtgacagagg 6481 tcagctggga tgcactccga ctgaactgga cgacaccgga cggtacttac gaccaattca 6541 caatccaagt gcaagaagcg gaccaagtcg aggaggcgca taatcttacg gtgccgggca 6601 gtctacgctc catggaaatc ccgggcctga gggcggggac gccgtatact gttacgctac 6661 atggcgaagt acgcggtcat tcgacacgtc cgttggccgt tgaagtcgtt accgaagacc 6721 tccctcaact cggtgatctg gcggtaagcg aagtgggttg ggacggcctg cgcctgaatt 6781 ggaccgcagc cgataacgca tacgagcatt tcgtaataca ggttcaggag gtaaacaaag 6841 tggaagccgc gcagaatctg accctcccgg gctcgctgcg tgcagtggat attcctggcc 6901 tggaagcagc cacaccgtat cgtgtcagca tatacggcgt catacgtgga tatcgaacgc 6961 cggtactgtc ggcggaagca tccacggcca aagagccaga aattggcaac ttgaacgtaa 7021 gtgacattac tcccgagtcg tttaacctta gttggatggc cacggatggg atcttcgaaa 7081 cctttacgat tgagattata gatagcaacc gcttgctgga gaccgtagaa tataacatca 7141 gcggcgcgga acgtactgcg cacatttcgg gcctgccacc gtctaccgac ttcatagtgt 7201 acctgtcggg cctggcgccg agtatccgca cgaagacgat cagcgcaacc gcaaccacag 7261 aggcgttgcc tttgctcgaa aacctgacca tcagtgatat caatccatac ggtttcacgg 7321 tctcgtggat ggcgagtgag aatgcatttg actccttttt agtgactgtc gtggactcag 7381 gtaaactttt agatcctcag gagtttactt tgagcggcac ccagcgcaaa ctggaactgc 7441 gtggactgat taccggcatc gggtatgagg tgatggtatc tgggttcacg cagggtcatc 7501 aaaccaaacc attacgggct gagatcgtca ctgaagcgga accggaggtc gataacctac 7561 tcgtctctga tgcaacgccg gacggcttcc gcttatcgtg gacagccgat gaaggcgtat 7621 tcgataactt tgtcctgaaa attcgcgaca ctaaaaaaca atctgaacct cttgaaatta 7681 cactgcttgc tccggaacgt acacgtgata ttactggcct gcgtgaagcg acggagtatg 7741 aaatcgaact gtatgggatt tcgaagggtc ggcgtagcca gactgtgtcg gcaattgcca 7801 cgaccgccat gggatccccg aaagaagtta tcttttcgga cattaccgag aacagtgcaa 7861 ccgtgtcatg gcgcgccccg acagcgcagg ttgagtcatt tcgtattaca tacgtcccaa 7921 ttaccggcgg cacccctagt atggttaccg ttgatggcac gaaaacgcag acgcgtttag 7981 ttaaactgat acccggtgtg gaatatttag tgagcatcat cgctatgaag ggttttgagg 8041 aatccgaacc tgtgtctggt tcgttcacga ccgctctcga tggtccatcc ggtctggtca 8101 cggcgaacat cacggacagc gaagctctcg cgagatggca gccggcaatt gcgaccgttg 8161 actcttatgt gatatcgtat accggggaga aagtgccaga aattacccgc accgtgagcg 8221 gaaacacagt ggaatacgct ctgactgatc tggaaccggc aaccgaatac accctgcgaa 8281 ttttcgccga gaaaggcccc caaaagagct ccacaataac tgcgaagttt accacggatt 8341 tagactctcc tcgcgatctc acagcgacag aagtccagag cgaaaccgca ctcttgacct 8401 ggcggccacc acgtgcgagt gtcaccggct atctgttagt ctacgagtct gtggatggta 8461 ccgtcaaaga agtaatagtc ggaccggata ccacctccta tagcctcgct gacctttccc 8521 cgtcgacaca ttacaccgct aaaattcagg ctttgaacgg ccccctgcgc tccaatatga 8581 ttcaaactat ctttaccact attggtctgc tgtatccgtt tccgaaagat tgctcccagg 8641 caatgttaaa tggcgacact acgtcgggcc tgtacactat ctatctaaat ggtgataagg 8701 cagaggcatt ggaagtgttt tgtgacatga cgtcggatgg tggagggtgg attgtctttt 8761 tacgccgcaa aaacgggcga gaaaacttct atcagaactg gaaggcctat gcggcagggt 8821 ttggggatcg gcgcgaggag ttttggctgg ggctggacaa tctgaacaaa attactgcac 8881 aaggccagta cgagctgcgt gttgatctgc gcgatcatgg tgaaaccgcg tttgcggtgt 8941 atgataaatt ctctgtaggc gatgcgaaaa ctcgttataa actgaaggta gaagggtatt 9001 cgggtacagc cggcgattct atggcgtacc acaatggtcg ttcgtttagc acgtttgata 9061 aagataccga cagtgccatt accaactgcg ctctttcata caaaggagct ttttggtatc 9121 gcaactgcca ccgcgtgaat ttaatgggcc gctacggaga caataatcat tcgcagggag 9181 ttaattggtt ccattggaag ggtcatgagc acagcattca gttcgcggag atgaagttgc 9241 gtccgagtaa ctttcggaat ctggaaggac gccgtaaacg cgcgtactag agtcacactg 9301 gctcaccttc gggtgggcct ttctgcgttt ata //