##gff-version 3
##sequence-region BBa_M1657 2664 9333
BBa_M1657	.	ribosome_entry_site	2664	2675	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1657	.	terminator	9293	9333	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1657	.	engineered_region	9293	9333	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1657	.	stop_codon	9326	9326	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1657	.	stem_loop	9300	9319	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1657	.	polyA_site	9320	9333	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1657	.	CDS	2682	9284	.	+	0	ID=BBa_M1653;Name=BBa_M1653
BBa_M1657	.	stop_codon	9285	9287	.	+	0	ID=annotation2214561;Name=stop;Parent=BBa_M1653
BBa_M1657	.	start_codon	2682	2684	.	+	0	ID=annotation2214560;Name=start;Parent=BBa_M1653
BBa_M1657	.	CDS	2685	9284	.	+	0	ID=annotation2214562;Name=Tenascin-C;Parent=BBa_M1653
>BBa_M1657
atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgacc
attacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaa
gatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccct
aagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcc
tgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatac
aaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaa
gctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaag
ttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattca
tgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagta
ggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggca
tctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacgg
tcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtg
tacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcacca
agtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacat
gaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgt
atcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggact
ggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaa
aggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccg
ttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacactt
ggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgag
ctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgag
gtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacg
agattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctc
tgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgt
tcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagcta
ttgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgt
gagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaa
gataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcagg
aatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaa
caaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccac
cttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtacca
ttccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggc
tgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaac
ttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagaaagaggggacatactagatggg
ggccatgacccaattactggccggtgttttcctggcgtttctggctctggcgacagaaggtggtgtactgaaaaaagtg
attcgccacaagcggcaaagcggagtaaatgcgacgcttccggaagagaatcagccggttgtgttcaaccatgtttata
acatcaaactgccggtgggttcccagtgttctgttgatttggagagtgcgagtggagaaaaggacctagcgccgccttc
agagccgtcggagtctttccaggaacatacagttgatggtgaaaatcaaattgtgtttacccaccgtatcaatatcccg
cgtcgcgcctgcggttgtgctgccgccccagacgttaaagagcttctgtcacgacttgaagaactggagaacttagtga
gcagtctgcgtgaacagtgcacggcgggtgccggctgctgcctccagccggcaacaggtcgcctggacacccgcccctt
ctgctcggggcgaggcaattttagcacggaaggttgcggctgcgtatgtgaacctgggtggaaaggtcctaattgttct
gaaccagaatgccctgggaattgtcatctgcgcgggcgctgcattgacggccagtgcatttgtgacgatggttttactg
gcgaggactgttcacaattggcctgcccatcagactgcaatgatcaaggtaagtgcgtgaatggcgtatgcatttgctt
cgaaggttatgcgggcgctgactgctctcgtgaaatctgtccagtaccctgctccgaagaacatggcacttgtgtggac
gggttatgtgtatgtcacgacggatttgccggcgatgattgtaataaacctctttgtctgaacaactgttataatcgcg
gtcgctgtgtagaaaatgaatgcgtatgtgatgaaggatttaccggtgaggattgcagtgaactgatttgtcctaacga
ttgctttgaccggggacgatgcatcaacggcacctgctactgtgaggagggcttcaccggtgaggactgcggtaaaccg
acctgtccgcacgcttgtcacacccaaggtcgctgcgaggagggccagtgtgtctgtgatgaaggcttcgccggcgttg
attgctccgaaaagcgctgcccagcggattgccataaccgggggcgttgcgttgatggtcggtgcgagtgcgacgacgg
tttcaccggagcggattgtggcgaactgaaatgcccgaacggctgctctggccacggccgttgtgtgaatggccagtgt
gtttgcgatgagggctataccggcgaagattgttctcaactgcgttgccccaatgactgccatagccgtgggcgctgtg
tcgaaggaaagtgtgtgtgtgagcaaggctttaaaggttatgattgctcagatatgagctgtcctaatgactgtcatca
gcacgggcgctgtgtaaatggtatgtgcgtttgtgatgatggctacaccggagaggattgccgcgatcgtcaatgtccg
cgtgactgttctaatcgcggtctgtgtgtggatggacaatgtgtgtgcgaagacggcttcacgggaccggattgtgcgg
aactctcttgcccaaacgactgtcacggtcaaggtcgctgtgtgaacggtcaatgcgtgtgtcatgaaggttttatggg
caaggattgtaaggaacagcgatgcccttccgattgccacggtcaaggacgttgtgtagatggccagtgcatctgccat
gaaggattcaccggtctcgattgcggtcagcactcctgcccgagcgactgtaacaacctcggccaatgtgtaagtggtc
gctgcatttgcaatgaaggttactcaggggaagactgtagcgaagttagcccgccgaaggatttggtggttacagaagt
caccgaggaaacggttaatctggcctgggacaatgaaatgcgggtaaccgaatatctggtagtttatacacccacccat
gagggcgggctggaaatgcagtttcgcgtaccgggtgatcaaacgagtaccattatccaggagttagaaccgggcgtgg
aatatttcatccgcgtcttcgccattctggaaaacaaaaagtccatccctgtatcagctcgcgttgcaacctacctgcc
ggccccggagggtctcaagtttaagagtatcaaagagacgagcgttgaagtcgaatgggacccactcgacattgctttc
gaaacgtgggaaattatctttcgtaacatgaataaggaggatgagggcgaaataaccaaaagcttgcgtcgcccggaga
cctcttatcgccaaacgggattggcgcctggccaggagtacgagatttctcttcatattgtgaaaaataatactcgcgg
tccaggcctcaagcgtgtgacgaccacgcgccttgacgcgccctcacagattgaagttaaggatgttactgacaccacg
gcgctgattacctggttcaaaccgcttgcagaaattgatggcatagaactgacctacggcattaaggatgtcccgggtg
accgtaccaccatagacctgactgaggatgagaaccagtatagtatcggtaatctgaaaccggataccgaatatgaggt
gagcctgatcagtcgccgtggagacatgtcgtctaacccggccaaagagacctttaccactggcctggatgcgccacgt
aatcttaggcgcgtatcgcaaacagacaactcaattacccttgaatggcgtaacggtaaagcggccattgattcgtatc
gaattaaatatgcaccgatttcaggcggcgaccacgcggaagttgatgttcctaaaagccagcaggcgaccacgaaaac
gaccctgacgggcctgcgcccgggaacggaatacggtattggcgtatctgcggtgaaagaagacaaggaatccaacccg
gcgaccattaacgcggcaacagaattggatactccaaaagatctacaagtaagtgaaactgctgaaacgagtctcactc
tactgtggaaaactccgctggccaaatttgatcggtatcgtctcaactacagtctgccaaccggacagtgggttggggt
acagttaccgcgtaacaccacatcatatgttttgcgcggcctggagccgggccaggaatacaatgtcttgctgacggcg
gagaaaggccgtcataaaagtaaaccggcgcgtgtcaaagcttcgaccgaacaggcaccagaactggaaaacctgacag
ttacggaggttgggtgggacggtttgcgcttgaactggaccgccgccgaccaggcatacgaacattttatcattcaggt
gcaggaagccaacaaggtagaagcagcccgcaatctgaccgtccctgggagcctgagagctgtggatattccggggctt
aaagccgcgacaccatatacggtgagtatatacggtgttatccagggctaccgtacgcctgttcttagcgctgaagcga
gcaccggcgaaacccctaacctgggcgaggtggtggtggcggaagttggctgggatgcgctgaaactcaactggacagc
ccctgaaggagcctacgaatatttctttattcaggtccaggaagctgatacagttgaggcggcacagaaccttacggtc
cccgggggtttgcgttctaccgatctcccgggactgaaggcagcgacgcactacaccattacgatcagaggggtcaccc
aggattttagtactactcctctgagcgtggaagtcctgaccgaagaagttccggatatgggtaatctaacagtgacaga
ggtcagctgggatgcactccgactgaactggacgacaccggacggtacttacgaccaattcacaatccaagtgcaagaa
gcggaccaagtcgaggaggcgcataatcttacggtgccgggcagtctacgctccatggaaatcccgggcctgagggcgg
ggacgccgtatactgttacgctacatggcgaagtacgcggtcattcgacacgtccgttggccgttgaagtcgttaccga
agacctccctcaactcggtgatctggcggtaagcgaagtgggttgggacggcctgcgcctgaattggaccgcagccgat
aacgcatacgagcatttcgtaatacaggttcaggaggtaaacaaagtggaagccgcgcagaatctgaccctcccgggct
cgctgcgtgcagtggatattcctggcctggaagcagccacaccgtatcgtgtcagcatatacggcgtcatacgtggata
tcgaacgccggtactgtcggcggaagcatccacggccaaagagccagaaattggcaacttgaacgtaagtgacattact
cccgagtcgtttaaccttagttggatggccacggatgggatcttcgaaacctttacgattgagattatagatagcaacc
gcttgctggagaccgtagaatataacatcagcggcgcggaacgtactgcgcacatttcgggcctgccaccgtctaccga
cttcatagtgtacctgtcgggcctggcgccgagtatccgcacgaagacgatcagcgcaaccgcaaccacagaggcgttg
cctttgctcgaaaacctgaccatcagtgatatcaatccatacggtttcacggtctcgtggatggcgagtgagaatgcat
ttgactcctttttagtgactgtcgtggactcaggtaaacttttagatcctcaggagtttactttgagcggcacccagcg
caaactggaactgcgtggactgattaccggcatcgggtatgaggtgatggtatctgggttcacgcagggtcatcaaacc
aaaccattacgggctgagatcgtcactgaagcggaaccggaggtcgataacctactcgtctctgatgcaacgccggacg
gcttccgcttatcgtggacagccgatgaaggcgtattcgataactttgtcctgaaaattcgcgacactaaaaaacaatc
tgaacctcttgaaattacactgcttgctccggaacgtacacgtgatattactggcctgcgtgaagcgacggagtatgaa
atcgaactgtatgggatttcgaagggtcggcgtagccagactgtgtcggcaattgccacgaccgccatgggatccccga
aagaagttatcttttcggacattaccgagaacagtgcaaccgtgtcatggcgcgccccgacagcgcaggttgagtcatt
tcgtattacatacgtcccaattaccggcggcacccctagtatggttaccgttgatggcacgaaaacgcagacgcgttta
gttaaactgatacccggtgtggaatatttagtgagcatcatcgctatgaagggttttgaggaatccgaacctgtgtctg
gttcgttcacgaccgctctcgatggtccatccggtctggtcacggcgaacatcacggacagcgaagctctcgcgagatg
gcagccggcaattgcgaccgttgactcttatgtgatatcgtataccggggagaaagtgccagaaattacccgcaccgtg
agcggaaacacagtggaatacgctctgactgatctggaaccggcaaccgaatacaccctgcgaattttcgccgagaaag
gcccccaaaagagctccacaataactgcgaagtttaccacggatttagactctcctcgcgatctcacagcgacagaagt
ccagagcgaaaccgcactcttgacctggcggccaccacgtgcgagtgtcaccggctatctgttagtctacgagtctgtg
gatggtaccgtcaaagaagtaatagtcggaccggataccacctcctatagcctcgctgacctttccccgtcgacacatt
acaccgctaaaattcaggctttgaacggccccctgcgctccaatatgattcaaactatctttaccactattggtctgct
gtatccgtttccgaaagattgctcccaggcaatgttaaatggcgacactacgtcgggcctgtacactatctatctaaat
ggtgataaggcagaggcattggaagtgttttgtgacatgacgtcggatggtggagggtggattgtctttttacgccgca
aaaacgggcgagaaaacttctatcagaactggaaggcctatgcggcagggtttggggatcggcgcgaggagttttggct
ggggctggacaatctgaacaaaattactgcacaaggccagtacgagctgcgtgttgatctgcgcgatcatggtgaaacc
gcgtttgcggtgtatgataaattctctgtaggcgatgcgaaaactcgttataaactgaaggtagaagggtattcgggta
cagccggcgattctatggcgtaccacaatggtcgttcgtttagcacgtttgataaagataccgacagtgccattaccaa
ctgcgctctttcatacaaaggagctttttggtatcgcaactgccaccgcgtgaatttaatgggccgctacggagacaat
aatcattcgcagggagttaattggttccattggaagggtcatgagcacagcattcagttcgcggagatgaagttgcgtc
cgagtaactttcggaatctggaaggacgccgtaaacgcgcgtactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata