LOCUS BBa_M1659 1464 bp DNA linear 24-Nov-2024 DEFINITION Composite part for lung cancer biomarker ACCESSION BBa_M1659 VERSION BBa_M1659.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_I712074 RBS 55..66 /label=BBa_J61100 misc_feature 75..104 /label=10x-Histidine Tag misc_feature 105..107 /label=Stop misc_feature 108..110 /label=Stop misc_feature 75..110 /label=BBa_K844000 misc_feature 117..119 /label=start misc_feature 1413..1415 /label=stop CDS 120..1412 /label=KRT17 CDS 117..1415 /label=BBa_M1655 stem_loop 1431..1450 /label=T7 TE misc_feature 1457..1457 /label=stop polyA_site 1451..1464 /label=polya misc_feature 1424..1464 /label=BBa_B0012 terminator 1424..1464 /label=BBa_B0012 ORIGIN 1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag 61 gggacatact agagcatcat caccatcacc accatcatca ccattaataa tactagatga 121 ccactagcat ccgtcaattt acctcgagca gcagtataaa aggctccagc ggcttgggag 181 gcggatcaag ccgcacaagc tgtcgtctga gcggtggcct gggagctggc tcatgccgtt 241 taggttctgc cggtggtttg ggtagcacgc tgggcggttc ctcgtacagt agctgttaca 301 gctttggtag cggcggcggc tacggatcaa gcttcggcgg ggtcgatggc ctgctcgctg 361 gcggagaaaa agccacgatg cagaacttaa acgaccgact cgccagctat ctggataagg 421 ttcgtgcact ggaagaagca aatactgaac tggaagttaa aatccgcgat tggtatcagc 481 gccaggcgcc gggcccagcg cgggattaca gccagtatta tcgaaccatt gaagagctcc 541 aaaacaaaat tctgactgcc acagttgata atgcgaatat tcttttacag attgataatg 601 cacgtctggc agctgacgac tttcgcacca aattcgagac cgaacaggca ctccgtctga 661 gtgtggaggc tgatatcaac gggcttcgac gcgtgttgga cgagttaacc ttagcgcgcg 721 cagacctaga aatgcagatt gaaaacctga aggaggagct cgcatatctc aaaaaaaatc 781 atgaagaaga aatgaatgcg ttgcgcggcc aggtcggcgg ggaaatcaat gttgagatgg 841 atgctgcccc gggtgtggat ctcagtcgca ttttaaatga aatgcgtgat cagtatgaga 901 aaatggcaga aaaaaaccgt aaagacgcgg aagattggtt tttctctaaa accgaagaat 961 tgaaccgcga agtcgcaacc aactctgaac tggttcagtc ggggaaaagc gaaatttccg 1021 aattacgtcg tacgatgcag gccctggaaa tcgaactaca atcgcagctg tcaatgaagg 1081 ccagcctcga aggcaatttg gccgaaaccg agaaccgcta ctgtgtacag ctgtcccaga 1141 tccaaggtct gattggttca gtcgaagagc aactggcgca gctcagatgc gaaatggaac 1201 aacagaacca ggaatataaa atcttacttg atgtgaaaac gcgcctggag caggaaatcg 1261 cgacgtatcg tcgcttactg gaaggggaag acgcccattt gacccagtac aaaaaggaac 1321 cagtgaccac tcgccaagtt cgcaccatcg tggaagaggt ccaggacggg aaagttatta 1381 gtagtcgcga acaagtgcat cagaccaccc gctagtacta gagtcacact ggctcacctt 1441 cgggtgggcc tttctgcgtt tata //