BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_M1659
1
BBa_M1659
Composite part for lung cancer biomarker
2013-04-04T11:00:00Z
2015-05-08T01:13:57Z
source is human genome which has been optimized according to e.coli genome.
this is the composite part with promoter and RBS and the coding area for a cancer biomarker present in lung epithelial bronchial fluid.
false
false
_768_
0
16364
9
Not in stock
false
it has a promoter sequence, ribosome binding site, histag, coding region and a terminator
Check for restriction enzyme sites.
false
Pratima labroo
component2214802
1
BBa_I712074
component2214812
1
BBa_B0012
component2214807
1
BBa_K844000
component2214803
1
BBa_J61100
component2214811
1
BBa_M1655
annotation2214802
1
BBa_I712074
range2214802
1
1
46
annotation2214811
1
BBa_M1655
range2214811
1
117
1415
annotation2214807
1
BBa_K844000
range2214807
1
75
110
annotation2214803
1
BBa_J61100
range2214803
1
55
66
annotation2214812
1
BBa_B0012
range2214812
1
1424
1464
BBa_K844000
1
BBa_K844000
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Constructed through oligonucleotide annealing
Released HQ 2013
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
false
false
_1104_
0
9404
9
In stock
true
none
false
Kathleen Miller
annotation2206607
1
10x-Histidine Tag
range2206607
1
1
30
annotation2206609
1
Stop
range2206609
1
34
36
annotation2206608
1
Stop
range2206608
1
31
33
BBa_M1655
1
BBa_M1655
Homo sapiens keratin 17 (KRT17)
2013-03-31T11:00:00Z
2015-05-08T01:13:57Z
human
Homo sapiens keratin 17 (KRT17) biomarker
false
false
_768_
0
16144
9
Not in stock
false
codon optimization and restriction enzyme sites were removed
false
whitney morgan
annotation2214572
1
stop
range2214572
1
1297
1299
annotation2214573
1
KRT17
range2214573
1
4
1296
annotation2214571
1
start
range2214571
1
1
3
BBa_M1655_sequence
1
atgaccactagcatccgtcaatttacctcgagcagcagtataaaaggctccagcggcttgggaggcggatcaagccgcacaagctgtcgtctgagcggtggcctgggagctggctcatgccgtttaggttctgccggtggtttgggtagcacgctgggcggttcctcgtacagtagctgttacagctttggtagcggcggcggctacggatcaagcttcggcggggtcgatggcctgctcgctggcggagaaaaagccacgatgcagaacttaaacgaccgactcgccagctatctggataaggttcgtgcactggaagaagcaaatactgaactggaagttaaaatccgcgattggtatcagcgccaggcgccgggcccagcgcgggattacagccagtattatcgaaccattgaagagctccaaaacaaaattctgactgccacagttgataatgcgaatattcttttacagattgataatgcacgtctggcagctgacgactttcgcaccaaattcgagaccgaacaggcactccgtctgagtgtggaggctgatatcaacgggcttcgacgcgtgttggacgagttaaccttagcgcgcgcagacctagaaatgcagattgaaaacctgaaggaggagctcgcatatctcaaaaaaaatcatgaagaagaaatgaatgcgttgcgcggccaggtcggcggggaaatcaatgttgagatggatgctgccccgggtgtggatctcagtcgcattttaaatgaaatgcgtgatcagtatgagaaaatggcagaaaaaaaccgtaaagacgcggaagattggtttttctctaaaaccgaagaattgaaccgcgaagtcgcaaccaactctgaactggttcagtcggggaaaagcgaaatttccgaattacgtcgtacgatgcaggccctggaaatcgaactacaatcgcagctgtcaatgaaggccagcctcgaaggcaatttggccgaaaccgagaaccgctactgtgtacagctgtcccagatccaaggtctgattggttcagtcgaagagcaactggcgcagctcagatgcgaaatggaacaacagaaccaggaatataaaatcttacttgatgtgaaaacgcgcctggagcaggaaatcgcgacgtatcgtcgcttactggaaggggaagacgcccatttgacccagtacaaaaaggaaccagtgaccactcgccaagttcgcaccatcgtggaagaggtccaggacgggaaagttattagtagtcgcgaacaagtgcatcagaccacccgctag
BBa_J61100_sequence
1
aaagaggggaca
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_K844000_sequence
1
catcatcaccatcaccaccatcatcaccattaataa
BBa_M1659_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagagcatcatcaccatcaccaccatcatcaccattaataatactagatgaccactagcatccgtcaatttacctcgagcagcagtataaaaggctccagcggcttgggaggcggatcaagccgcacaagctgtcgtctgagcggtggcctgggagctggctcatgccgtttaggttctgccggtggtttgggtagcacgctgggcggttcctcgtacagtagctgttacagctttggtagcggcggcggctacggatcaagcttcggcggggtcgatggcctgctcgctggcggagaaaaagccacgatgcagaacttaaacgaccgactcgccagctatctggataaggttcgtgcactggaagaagcaaatactgaactggaagttaaaatccgcgattggtatcagcgccaggcgccgggcccagcgcgggattacagccagtattatcgaaccattgaagagctccaaaacaaaattctgactgccacagttgataatgcgaatattcttttacagattgataatgcacgtctggcagctgacgactttcgcaccaaattcgagaccgaacaggcactccgtctgagtgtggaggctgatatcaacgggcttcgacgcgtgttggacgagttaaccttagcgcgcgcagacctagaaatgcagattgaaaacctgaaggaggagctcgcatatctcaaaaaaaatcatgaagaagaaatgaatgcgttgcgcggccaggtcggcggggaaatcaatgttgagatggatgctgccccgggtgtggatctcagtcgcattttaaatgaaatgcgtgatcagtatgagaaaatggcagaaaaaaaccgtaaagacgcggaagattggtttttctctaaaaccgaagaattgaaccgcgaagtcgcaaccaactctgaactggttcagtcggggaaaagcgaaatttccgaattacgtcgtacgatgcaggccctggaaatcgaactacaatcgcagctgtcaatgaaggccagcctcgaaggcaatttggccgaaaccgagaaccgctactgtgtacagctgtcccagatccaaggtctgattggttcagtcgaagagcaactggcgcagctcagatgcgaaatggaacaacagaaccaggaatataaaatcttacttgatgtgaaaacgcgcctggagcaggaaatcgcgacgtatcgtcgcttactggaaggggaagacgcccatttgacccagtacaaaaaggaaccagtgaccactcgccaagttcgcaccatcgtggaagaggtccaggacgggaaagttattagtagtcgcgaacaagtgcatcagaccacccgctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z