BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J61100 1 BBa_J61100 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A {{JCA_Arkin_RBSFamily}} false true _95_ 0 483 95 In stock false N/A true John Anderson BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_M1659 1 BBa_M1659 Composite part for lung cancer biomarker 2013-04-04T11:00:00Z 2015-05-08T01:13:57Z source is human genome which has been optimized according to e.coli genome. this is the composite part with promoter and RBS and the coding area for a cancer biomarker present in lung epithelial bronchial fluid. false false _768_ 0 16364 9 Not in stock false it has a promoter sequence, ribosome binding site, histag, coding region and a terminator Check for restriction enzyme sites. false Pratima labroo component2214802 1 BBa_I712074 component2214812 1 BBa_B0012 component2214807 1 BBa_K844000 component2214803 1 BBa_J61100 component2214811 1 BBa_M1655 annotation2214802 1 BBa_I712074 range2214802 1 1 46 annotation2214811 1 BBa_M1655 range2214811 1 117 1415 annotation2214807 1 BBa_K844000 range2214807 1 75 110 annotation2214803 1 BBa_J61100 range2214803 1 55 66 annotation2214812 1 BBa_B0012 range2214812 1 1424 1464 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206609 1 Stop range2206609 1 34 36 annotation2206608 1 Stop range2206608 1 31 33 BBa_M1655 1 BBa_M1655 Homo sapiens keratin 17 (KRT17) 2013-03-31T11:00:00Z 2015-05-08T01:13:57Z human Homo sapiens keratin 17 (KRT17) biomarker false false _768_ 0 16144 9 Not in stock false codon optimization and restriction enzyme sites were removed false whitney morgan annotation2214572 1 stop range2214572 1 1297 1299 annotation2214573 1 KRT17 range2214573 1 4 1296 annotation2214571 1 start range2214571 1 1 3 BBa_M1655_sequence 1 atgaccactagcatccgtcaatttacctcgagcagcagtataaaaggctccagcggcttgggaggcggatcaagccgcacaagctgtcgtctgagcggtggcctgggagctggctcatgccgtttaggttctgccggtggtttgggtagcacgctgggcggttcctcgtacagtagctgttacagctttggtagcggcggcggctacggatcaagcttcggcggggtcgatggcctgctcgctggcggagaaaaagccacgatgcagaacttaaacgaccgactcgccagctatctggataaggttcgtgcactggaagaagcaaatactgaactggaagttaaaatccgcgattggtatcagcgccaggcgccgggcccagcgcgggattacagccagtattatcgaaccattgaagagctccaaaacaaaattctgactgccacagttgataatgcgaatattcttttacagattgataatgcacgtctggcagctgacgactttcgcaccaaattcgagaccgaacaggcactccgtctgagtgtggaggctgatatcaacgggcttcgacgcgtgttggacgagttaaccttagcgcgcgcagacctagaaatgcagattgaaaacctgaaggaggagctcgcatatctcaaaaaaaatcatgaagaagaaatgaatgcgttgcgcggccaggtcggcggggaaatcaatgttgagatggatgctgccccgggtgtggatctcagtcgcattttaaatgaaatgcgtgatcagtatgagaaaatggcagaaaaaaaccgtaaagacgcggaagattggtttttctctaaaaccgaagaattgaaccgcgaagtcgcaaccaactctgaactggttcagtcggggaaaagcgaaatttccgaattacgtcgtacgatgcaggccctggaaatcgaactacaatcgcagctgtcaatgaaggccagcctcgaaggcaatttggccgaaaccgagaaccgctactgtgtacagctgtcccagatccaaggtctgattggttcagtcgaagagcaactggcgcagctcagatgcgaaatggaacaacagaaccaggaatataaaatcttacttgatgtgaaaacgcgcctggagcaggaaatcgcgacgtatcgtcgcttactggaaggggaagacgcccatttgacccagtacaaaaaggaaccagtgaccactcgccaagttcgcaccatcgtggaagaggtccaggacgggaaagttattagtagtcgcgaacaagtgcatcagaccacccgctag BBa_J61100_sequence 1 aaagaggggaca BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_M1659_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagagcatcatcaccatcaccaccatcatcaccattaataatactagatgaccactagcatccgtcaatttacctcgagcagcagtataaaaggctccagcggcttgggaggcggatcaagccgcacaagctgtcgtctgagcggtggcctgggagctggctcatgccgtttaggttctgccggtggtttgggtagcacgctgggcggttcctcgtacagtagctgttacagctttggtagcggcggcggctacggatcaagcttcggcggggtcgatggcctgctcgctggcggagaaaaagccacgatgcagaacttaaacgaccgactcgccagctatctggataaggttcgtgcactggaagaagcaaatactgaactggaagttaaaatccgcgattggtatcagcgccaggcgccgggcccagcgcgggattacagccagtattatcgaaccattgaagagctccaaaacaaaattctgactgccacagttgataatgcgaatattcttttacagattgataatgcacgtctggcagctgacgactttcgcaccaaattcgagaccgaacaggcactccgtctgagtgtggaggctgatatcaacgggcttcgacgcgtgttggacgagttaaccttagcgcgcgcagacctagaaatgcagattgaaaacctgaaggaggagctcgcatatctcaaaaaaaatcatgaagaagaaatgaatgcgttgcgcggccaggtcggcggggaaatcaatgttgagatggatgctgccccgggtgtggatctcagtcgcattttaaatgaaatgcgtgatcagtatgagaaaatggcagaaaaaaaccgtaaagacgcggaagattggtttttctctaaaaccgaagaattgaaccgcgaagtcgcaaccaactctgaactggttcagtcggggaaaagcgaaatttccgaattacgtcgtacgatgcaggccctggaaatcgaactacaatcgcagctgtcaatgaaggccagcctcgaaggcaatttggccgaaaccgagaaccgctactgtgtacagctgtcccagatccaaggtctgattggttcagtcgaagagcaactggcgcagctcagatgcgaaatggaacaacagaaccaggaatataaaatcttacttgatgtgaaaacgcgcctggagcaggaaatcgcgacgtatcgtcgcttactggaaggggaagacgcccatttgacccagtacaaaaaggaaccagtgaccactcgccaagttcgcaccatcgtggaagaggtccaggacgggaaagttattagtagtcgcgaacaagtgcatcagaccacccgctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z