LOCUS BBa_M1660 8297 bp DNA linear 17-May-2024 DEFINITION Lung cancer biosensor - early detection ACCESSION BBa_M1660 VERSION BBa_M1660.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_I712074 RBS 55..66 /label=BBa_J61101 misc_feature 75..104 /label=10x-Histidine Tag misc_feature 105..107 /label=Stop misc_feature 108..110 /label=Stop misc_feature 75..110 /label=BBa_K844000 misc_feature 117..119 /label=start misc_feature 6720..6722 /label=stop CDS 120..6719 /label=Tenascin-C CDS 117..6722 /label=BBa_M1653 stem_loop 6738..6757 /label=T7 TE misc_feature 6764..6764 /label=stop polyA_site 6758..6771 /label=polya misc_feature 6731..6771 /label=BBa_B0012 terminator 6731..6771 /label=BBa_B0012 stem_loop 6792..6814 /label=stem_loop misc_feature 6780..6825 /label=BBa_B0011 terminator 6780..6825 /label=BBa_B0011 promoter 6834..6879 /label=BBa_I712074 RBS 6888..6899 /label=BBa_J61103 misc_feature 6908..6937 /label=10x-Histidine Tag misc_feature 6938..6940 /label=Stop misc_feature 6941..6943 /label=Stop misc_feature 6908..6943 /label=BBa_K844000 misc_feature 6950..6952 /label=start misc_feature 8246..8248 /label=stop CDS 6953..8245 /label=KRT17 CDS 6950..8248 /label=BBa_M1655 stem_loop 8264..8283 /label=T7 TE misc_feature 8290..8290 /label=stop polyA_site 8284..8297 /label=polya misc_feature 8257..8297 /label=BBa_B0012 terminator 8257..8297 /label=BBa_B0012 ORIGIN 1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagac 61 aggacctact agagcatcat caccatcacc accatcatca ccattaataa tactagatgg 121 gggccatgac ccaattactg gccggtgttt tcctggcgtt tctggctctg gcgacagaag 181 gtggtgtact gaaaaaagtg attcgccaca agcggcaaag cggagtaaat gcgacgcttc 241 cggaagagaa tcagccggtt gtgttcaacc atgtttataa catcaaactg ccggtgggtt 301 cccagtgttc tgttgatttg gagagtgcga gtggagaaaa ggacctagcg ccgccttcag 361 agccgtcgga gtctttccag gaacatacag ttgatggtga aaatcaaatt gtgtttaccc 421 accgtatcaa tatcccgcgt cgcgcctgcg gttgtgctgc cgccccagac gttaaagagc 481 ttctgtcacg acttgaagaa ctggagaact tagtgagcag tctgcgtgaa cagtgcacgg 541 cgggtgccgg ctgctgcctc cagccggcaa caggtcgcct ggacacccgc cccttctgct 601 cggggcgagg caattttagc acggaaggtt gcggctgcgt atgtgaacct gggtggaaag 661 gtcctaattg ttctgaacca gaatgccctg ggaattgtca tctgcgcggg cgctgcattg 721 acggccagtg catttgtgac gatggtttta ctggcgagga ctgttcacaa ttggcctgcc 781 catcagactg caatgatcaa ggtaagtgcg tgaatggcgt atgcatttgc ttcgaaggtt 841 atgcgggcgc tgactgctct cgtgaaatct gtccagtacc ctgctccgaa gaacatggca 901 cttgtgtgga cgggttatgt gtatgtcacg acggatttgc cggcgatgat tgtaataaac 961 ctctttgtct gaacaactgt tataatcgcg gtcgctgtgt agaaaatgaa tgcgtatgtg 1021 atgaaggatt taccggtgag gattgcagtg aactgatttg tcctaacgat tgctttgacc 1081 ggggacgatg catcaacggc acctgctact gtgaggaggg cttcaccggt gaggactgcg 1141 gtaaaccgac ctgtccgcac gcttgtcaca cccaaggtcg ctgcgaggag ggccagtgtg 1201 tctgtgatga aggcttcgcc ggcgttgatt gctccgaaaa gcgctgccca gcggattgcc 1261 ataaccgggg gcgttgcgtt gatggtcggt gcgagtgcga cgacggtttc accggagcgg 1321 attgtggcga actgaaatgc ccgaacggct gctctggcca cggccgttgt gtgaatggcc 1381 agtgtgtttg cgatgagggc tataccggcg aagattgttc tcaactgcgt tgccccaatg 1441 actgccatag ccgtgggcgc tgtgtcgaag gaaagtgtgt gtgtgagcaa ggctttaaag 1501 gttatgattg ctcagatatg agctgtccta atgactgtca tcagcacggg cgctgtgtaa 1561 atggtatgtg cgtttgtgat gatggctaca ccggagagga ttgccgcgat cgtcaatgtc 1621 cgcgtgactg ttctaatcgc ggtctgtgtg tggatggaca atgtgtgtgc gaagacggct 1681 tcacgggacc ggattgtgcg gaactctctt gcccaaacga ctgtcacggt caaggtcgct 1741 gtgtgaacgg tcaatgcgtg tgtcatgaag gttttatggg caaggattgt aaggaacagc 1801 gatgcccttc cgattgccac ggtcaaggac gttgtgtaga tggccagtgc atctgccatg 1861 aaggattcac cggtctcgat tgcggtcagc actcctgccc gagcgactgt aacaacctcg 1921 gccaatgtgt aagtggtcgc tgcatttgca atgaaggtta ctcaggggaa gactgtagcg 1981 aagttagccc gccgaaggat ttggtggtta cagaagtcac cgaggaaacg gttaatctgg 2041 cctgggacaa tgaaatgcgg gtaaccgaat atctggtagt ttatacaccc acccatgagg 2101 gcgggctgga aatgcagttt cgcgtaccgg gtgatcaaac gagtaccatt atccaggagt 2161 tagaaccggg cgtggaatat ttcatccgcg tcttcgccat tctggaaaac aaaaagtcca 2221 tccctgtatc agctcgcgtt gcaacctacc tgccggcccc ggagggtctc aagtttaaga 2281 gtatcaaaga gacgagcgtt gaagtcgaat gggacccact cgacattgct ttcgaaacgt 2341 gggaaattat ctttcgtaac atgaataagg aggatgaggg cgaaataacc aaaagcttgc 2401 gtcgcccgga gacctcttat cgccaaacgg gattggcgcc tggccaggag tacgagattt 2461 ctcttcatat tgtgaaaaat aatactcgcg gtccaggcct caagcgtgtg acgaccacgc 2521 gccttgacgc gccctcacag attgaagtta aggatgttac tgacaccacg gcgctgatta 2581 cctggttcaa accgcttgca gaaattgatg gcatagaact gacctacggc attaaggatg 2641 tcccgggtga ccgtaccacc atagacctga ctgaggatga gaaccagtat agtatcggta 2701 atctgaaacc ggataccgaa tatgaggtga gcctgatcag tcgccgtgga gacatgtcgt 2761 ctaacccggc caaagagacc tttaccactg gcctggatgc gccacgtaat cttaggcgcg 2821 tatcgcaaac agacaactca attacccttg aatggcgtaa cggtaaagcg gccattgatt 2881 cgtatcgaat taaatatgca ccgatttcag gcggcgacca cgcggaagtt gatgttccta 2941 aaagccagca ggcgaccacg aaaacgaccc tgacgggcct gcgcccggga acggaatacg 3001 gtattggcgt atctgcggtg aaagaagaca aggaatccaa cccggcgacc attaacgcgg 3061 caacagaatt ggatactcca aaagatctac aagtaagtga aactgctgaa acgagtctca 3121 ctctactgtg gaaaactccg ctggccaaat ttgatcggta tcgtctcaac tacagtctgc 3181 caaccggaca gtgggttggg gtacagttac cgcgtaacac cacatcatat gttttgcgcg 3241 gcctggagcc gggccaggaa tacaatgtct tgctgacggc ggagaaaggc cgtcataaaa 3301 gtaaaccggc gcgtgtcaaa gcttcgaccg aacaggcacc agaactggaa aacctgacag 3361 ttacggaggt tgggtgggac ggtttgcgct tgaactggac cgccgccgac caggcatacg 3421 aacattttat cattcaggtg caggaagcca acaaggtaga agcagcccgc aatctgaccg 3481 tccctgggag cctgagagct gtggatattc cggggcttaa agccgcgaca ccatatacgg 3541 tgagtatata cggtgttatc cagggctacc gtacgcctgt tcttagcgct gaagcgagca 3601 ccggcgaaac ccctaacctg ggcgaggtgg tggtggcgga agttggctgg gatgcgctga 3661 aactcaactg gacagcccct gaaggagcct acgaatattt ctttattcag gtccaggaag 3721 ctgatacagt tgaggcggca cagaacctta cggtccccgg gggtttgcgt tctaccgatc 3781 tcccgggact gaaggcagcg acgcactaca ccattacgat cagaggggtc acccaggatt 3841 ttagtactac tcctctgagc gtggaagtcc tgaccgaaga agttccggat atgggtaatc 3901 taacagtgac agaggtcagc tgggatgcac tccgactgaa ctggacgaca ccggacggta 3961 cttacgacca attcacaatc caagtgcaag aagcggacca agtcgaggag gcgcataatc 4021 ttacggtgcc gggcagtcta cgctccatgg aaatcccggg cctgagggcg gggacgccgt 4081 atactgttac gctacatggc gaagtacgcg gtcattcgac acgtccgttg gccgttgaag 4141 tcgttaccga agacctccct caactcggtg atctggcggt aagcgaagtg ggttgggacg 4201 gcctgcgcct gaattggacc gcagccgata acgcatacga gcatttcgta atacaggttc 4261 aggaggtaaa caaagtggaa gccgcgcaga atctgaccct cccgggctcg ctgcgtgcag 4321 tggatattcc tggcctggaa gcagccacac cgtatcgtgt cagcatatac ggcgtcatac 4381 gtggatatcg aacgccggta ctgtcggcgg aagcatccac ggccaaagag ccagaaattg 4441 gcaacttgaa cgtaagtgac attactcccg agtcgtttaa ccttagttgg atggccacgg 4501 atgggatctt cgaaaccttt acgattgaga ttatagatag caaccgcttg ctggagaccg 4561 tagaatataa catcagcggc gcggaacgta ctgcgcacat ttcgggcctg ccaccgtcta 4621 ccgacttcat agtgtacctg tcgggcctgg cgccgagtat ccgcacgaag acgatcagcg 4681 caaccgcaac cacagaggcg ttgcctttgc tcgaaaacct gaccatcagt gatatcaatc 4741 catacggttt cacggtctcg tggatggcga gtgagaatgc atttgactcc tttttagtga 4801 ctgtcgtgga ctcaggtaaa cttttagatc ctcaggagtt tactttgagc ggcacccagc 4861 gcaaactgga actgcgtgga ctgattaccg gcatcgggta tgaggtgatg gtatctgggt 4921 tcacgcaggg tcatcaaacc aaaccattac gggctgagat cgtcactgaa gcggaaccgg 4981 aggtcgataa cctactcgtc tctgatgcaa cgccggacgg cttccgctta tcgtggacag 5041 ccgatgaagg cgtattcgat aactttgtcc tgaaaattcg cgacactaaa aaacaatctg 5101 aacctcttga aattacactg cttgctccgg aacgtacacg tgatattact ggcctgcgtg 5161 aagcgacgga gtatgaaatc gaactgtatg ggatttcgaa gggtcggcgt agccagactg 5221 tgtcggcaat tgccacgacc gccatgggat ccccgaaaga agttatcttt tcggacatta 5281 ccgagaacag tgcaaccgtg tcatggcgcg ccccgacagc gcaggttgag tcatttcgta 5341 ttacatacgt cccaattacc ggcggcaccc ctagtatggt taccgttgat ggcacgaaaa 5401 cgcagacgcg tttagttaaa ctgatacccg gtgtggaata tttagtgagc atcatcgcta 5461 tgaagggttt tgaggaatcc gaacctgtgt ctggttcgtt cacgaccgct ctcgatggtc 5521 catccggtct ggtcacggcg aacatcacgg acagcgaagc tctcgcgaga tggcagccgg 5581 caattgcgac cgttgactct tatgtgatat cgtataccgg ggagaaagtg ccagaaatta 5641 cccgcaccgt gagcggaaac acagtggaat acgctctgac tgatctggaa ccggcaaccg 5701 aatacaccct gcgaattttc gccgagaaag gcccccaaaa gagctccaca ataactgcga 5761 agtttaccac ggatttagac tctcctcgcg atctcacagc gacagaagtc cagagcgaaa 5821 ccgcactctt gacctggcgg ccaccacgtg cgagtgtcac cggctatctg ttagtctacg 5881 agtctgtgga tggtaccgtc aaagaagtaa tagtcggacc ggataccacc tcctatagcc 5941 tcgctgacct ttccccgtcg acacattaca ccgctaaaat tcaggctttg aacggccccc 6001 tgcgctccaa tatgattcaa actatcttta ccactattgg tctgctgtat ccgtttccga 6061 aagattgctc ccaggcaatg ttaaatggcg acactacgtc gggcctgtac actatctatc 6121 taaatggtga taaggcagag gcattggaag tgttttgtga catgacgtcg gatggtggag 6181 ggtggattgt ctttttacgc cgcaaaaacg ggcgagaaaa cttctatcag aactggaagg 6241 cctatgcggc agggtttggg gatcggcgcg aggagttttg gctggggctg gacaatctga 6301 acaaaattac tgcacaaggc cagtacgagc tgcgtgttga tctgcgcgat catggtgaaa 6361 ccgcgtttgc ggtgtatgat aaattctctg taggcgatgc gaaaactcgt tataaactga 6421 aggtagaagg gtattcgggt acagccggcg attctatggc gtaccacaat ggtcgttcgt 6481 ttagcacgtt tgataaagat accgacagtg ccattaccaa ctgcgctctt tcatacaaag 6541 gagctttttg gtatcgcaac tgccaccgcg tgaatttaat gggccgctac ggagacaata 6601 atcattcgca gggagttaat tggttccatt ggaagggtca tgagcacagc attcagttcg 6661 cggagatgaa gttgcgtccg agtaactttc ggaatctgga aggacgccgt aaacgcgcgt 6721 agtactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 6781 gagaatataa aaagccagat tattaatccg gcttttttat tattttacta gagtaatacg 6841 actcactata gggaatacaa gctacttgtt ctttttgcat actagagaaa gattagacat 6901 actagagcat catcaccatc accaccatca tcaccattaa taatactaga tgaccactag 6961 catccgtcaa tttacctcga gcagcagtat aaaaggctcc agcggcttgg gaggcggatc 7021 aagccgcaca agctgtcgtc tgagcggtgg cctgggagct ggctcatgcc gtttaggttc 7081 tgccggtggt ttgggtagca cgctgggcgg ttcctcgtac agtagctgtt acagctttgg 7141 tagcggcggc ggctacggat caagcttcgg cggggtcgat ggcctgctcg ctggcggaga 7201 aaaagccacg atgcagaact taaacgaccg actcgccagc tatctggata aggttcgtgc 7261 actggaagaa gcaaatactg aactggaagt taaaatccgc gattggtatc agcgccaggc 7321 gccgggccca gcgcgggatt acagccagta ttatcgaacc attgaagagc tccaaaacaa 7381 aattctgact gccacagttg ataatgcgaa tattctttta cagattgata atgcacgtct 7441 ggcagctgac gactttcgca ccaaattcga gaccgaacag gcactccgtc tgagtgtgga 7501 ggctgatatc aacgggcttc gacgcgtgtt ggacgagtta accttagcgc gcgcagacct 7561 agaaatgcag attgaaaacc tgaaggagga gctcgcatat ctcaaaaaaa atcatgaaga 7621 agaaatgaat gcgttgcgcg gccaggtcgg cggggaaatc aatgttgaga tggatgctgc 7681 cccgggtgtg gatctcagtc gcattttaaa tgaaatgcgt gatcagtatg agaaaatggc 7741 agaaaaaaac cgtaaagacg cggaagattg gtttttctct aaaaccgaag aattgaaccg 7801 cgaagtcgca accaactctg aactggttca gtcggggaaa agcgaaattt ccgaattacg 7861 tcgtacgatg caggccctgg aaatcgaact acaatcgcag ctgtcaatga aggccagcct 7921 cgaaggcaat ttggccgaaa ccgagaaccg ctactgtgta cagctgtccc agatccaagg 7981 tctgattggt tcagtcgaag agcaactggc gcagctcaga tgcgaaatgg aacaacagaa 8041 ccaggaatat aaaatcttac ttgatgtgaa aacgcgcctg gagcaggaaa tcgcgacgta 8101 tcgtcgctta ctggaagggg aagacgccca tttgacccag tacaaaaagg aaccagtgac 8161 cactcgccaa gttcgcacca tcgtggaaga ggtccaggac gggaaagtta ttagtagtcg 8221 cgaacaagtg catcagacca cccgctagta ctagagtcac actggctcac cttcgggtgg 8281 gcctttctgc gtttata //