BBa_M1660
type: ComponentDefinition
persistent identity: BBa M1660
display id: BBa_M1660
version: 1
was generated by: iGEM to SBOL conversion
description: Lung cancer biosensor - early detection
created: 2013-04-08T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Human
owned by: james
owned by: myers
mutable description: Biomakers for lung cancer detection in human epithelial-lining fluid
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16148
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part can be used to introduce a delay between induction and expression of the Human tenascin-C mRNA and Homo sapiens keratin 17 (KRT17) gene.
star: false
creator: Guevara Che Nyendu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J61101
component: BBa_I712074
component: BBa_B0011
component: BBa_B0012
component: BBa_M1653
component: BBa_M1655
component: BBa_K844000
component: BBa_J61103
sequence annotation: BBa_I712074
sequence annotation: BBa_J61101
sequence annotation: BBa_K844000
sequence annotation: BBa_M1653
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_J61103
sequence annotation: BBa_M1655
sequence: https://synbiohub.org/public/igem/BBa_M1660_sequence/1

BBa_J61101
type: Component
persistent identity: component2214817
display id: component2214817
version: 1
top level: BBa_M1660
definition: BBa_J61101
access: public

BBa_I712074
type: Component
persistent identity: component2214816
display id: component2214816
version: 1
top level: BBa_M1660
definition: BBa_I712074
access: public

BBa_I712074
type: Component
persistent identity: component2214832
display id: component2214832
version: 1
top level: BBa_M1660
definition: BBa_I712074
access: public

BBa_B0011
type: Component
persistent identity: component2214830
display id: component2214830
version: 1
top level: BBa_M1660
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component2214826
display id: component2214826
version: 1
top level: BBa_M1660
definition: BBa_B0012
access: public

BBa_M1653
type: Component
persistent identity: component2214825
display id: component2214825
version: 1
top level: BBa_M1660
definition: BBa_M1653
access: public

BBa_B0012
type: Component
persistent identity: component2214842
display id: component2214842
version: 1
top level: BBa_M1660
definition: BBa_B0012
access: public

BBa_M1655
type: Component
persistent identity: component2214841
display id: component2214841
version: 1
top level: BBa_M1660
definition: BBa_M1655
access: public

BBa_K844000
type: Component
persistent identity: component2214837
display id: component2214837
version: 1
top level: BBa_M1660
definition: BBa_K844000
access: public

BBa_J61103
type: Component
persistent identity: component2214833
display id: component2214833
version: 1
top level: BBa_M1660
definition: BBa_J61103
access: public

BBa_K844000
type: Component
persistent identity: component2214821
display id: component2214821
version: 1
top level: BBa_M1660
definition: BBa_K844000
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2214816
display id: annotation2214816
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214816/range2214816/1
component: BBa_I712074

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type: Range
persistent identity: range2214816
display id: range2214816
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2214817
display id: annotation2214817
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214817/range2214817/1
component: BBa_J61101

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type: Range
persistent identity: range2214817
display id: range2214817
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 55
end: 66
orientation: inline

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2214821
display id: annotation2214821
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214821/range2214821/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_M1660/annotation2214821/range2214821/1
type: Range
persistent identity: range2214821
display id: range2214821
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 75
end: 110
orientation: inline

BBa_M1653
type: SequenceAnnotation
persistent identity: annotation2214825
display id: annotation2214825
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214825/range2214825/1
component: BBa_M1653

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type: Range
persistent identity: range2214825
display id: range2214825
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 117
end: 6722
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2214826
display id: annotation2214826
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214826/range2214826/1
component: BBa_B0012

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type: Range
persistent identity: range2214826
display id: range2214826
version: 1
top level: BBa_M1660
direction: direction/forward
start: 6731
end: 6771
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2214830
display id: annotation2214830
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214830/range2214830/1
component: BBa_B0011

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type: Range
persistent identity: range2214830
display id: range2214830
version: 1
top level: BBa_M1660
direction: direction/forward
start: 6780
end: 6825
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2214832
display id: annotation2214832
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214832/range2214832/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_M1660/annotation2214832/range2214832/1
type: Range
persistent identity: range2214832
display id: range2214832
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 6834
end: 6879
orientation: inline

BBa_J61103
type: SequenceAnnotation
persistent identity: annotation2214833
display id: annotation2214833
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214833/range2214833/1
component: BBa_J61103

https://synbiohub.org/public/igem/BBa_M1660/annotation2214833/range2214833/1
type: Range
persistent identity: range2214833
display id: range2214833
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 6888
end: 6899
orientation: inline

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2214837
display id: annotation2214837
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214837/range2214837/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_M1660/annotation2214837/range2214837/1
type: Range
persistent identity: range2214837
display id: range2214837
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 6908
end: 6943
orientation: inline

BBa_M1655
type: SequenceAnnotation
persistent identity: annotation2214841
display id: annotation2214841
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214841/range2214841/1
component: BBa_M1655

https://synbiohub.org/public/igem/BBa_M1660/annotation2214841/range2214841/1
type: Range
persistent identity: range2214841
display id: range2214841
version: 1
top level: BBa_M1660
direction: direction/reverse
start: 6950
end: 8248
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2214842
display id: annotation2214842
version: 1
top level: BBa_M1660
location: https://synbiohub.org/public/igem/BBa_M1660/annotation2214842/range2214842/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1660/annotation2214842/range2214842/1
type: Range
persistent identity: range2214842
display id: range2214842
version: 1
top level: BBa_M1660
direction: direction/forward
start: 8257
end: 8297
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: fix
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: iGEM to SBOL conversion
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Kathleen Miller
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: 10x-Histidine Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
top level: BBa_K844000
direction: direction/forward
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
top level: BBa_K844000
direction: direction/forward
start: 31
end: 33
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
top level: BBa_K844000
direction: direction/forward
start: 34
end: 36
orientation: inline

BBa_M1653
type: ComponentDefinition
persistent identity: BBa M1653
display id: BBa_M1653
version: 1
was generated by: iGEM to SBOL conversion
description: Human tenascin-C mRNA
created: 2013-03-27T12:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Human source
owned by: james
owned by: myers
mutable description: An anti-marker that could be used to recognize the presence of lung cancer.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16132
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Potential affects of restriction enzymes.
star: false
creator: Howard Cordingley
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: Tenascin-C
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M1653_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2214560
display id: annotation2214560
version: 1
top level: BBa_M1653
location: https://synbiohub.org/public/igem/BBa_M1653/annotation2214560/range2214560/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1653/annotation2214560/range2214560/1
type: Range
persistent identity: range2214560
display id: range2214560
version: 1
top level: BBa_M1653
direction: direction/forward
start: 1
end: 3
orientation: inline

Tenascin-C
type: SequenceAnnotation
persistent identity: annotation2214562
display id: annotation2214562
version: 1
top level: BBa_M1653
location: https://synbiohub.org/public/igem/BBa_M1653/annotation2214562/range2214562/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1653/annotation2214562/range2214562/1
type: Range
persistent identity: range2214562
display id: range2214562
version: 1
top level: BBa_M1653
direction: direction/forward
start: 4
end: 6603
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2214561
display id: annotation2214561
version: 1
top level: BBa_M1653
location: https://synbiohub.org/public/igem/BBa_M1653/annotation2214561/range2214561/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1653/annotation2214561/range2214561/1
type: Range
persistent identity: range2214561
display id: range2214561
version: 1
top level: BBa_M1653
direction: direction/forward
start: 6604
end: 6606
orientation: inline

BBa_M1655
type: ComponentDefinition
persistent identity: BBa M1655
display id: BBa_M1655
version: 1
was generated by: iGEM to SBOL conversion
description: Homo sapiens keratin 17 (KRT17)
created: 2013-03-31T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: human
owned by: james
owned by: myers
mutable description: Homo sapiens keratin 17 (KRT17) biomarker
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16144
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: codon optimization and restriction enzyme sites were removed
star: false
creator: whitney morgan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: KRT17
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M1655_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2214571
display id: annotation2214571
version: 1
top level: BBa_M1655
location: https://synbiohub.org/public/igem/BBa_M1655/annotation2214571/range2214571/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1655/annotation2214571/range2214571/1
type: Range
persistent identity: range2214571
display id: range2214571
version: 1
top level: BBa_M1655
direction: direction/forward
start: 1
end: 3
orientation: inline

KRT17
type: SequenceAnnotation
persistent identity: annotation2214573
display id: annotation2214573
version: 1
top level: BBa_M1655
location: https://synbiohub.org/public/igem/BBa_M1655/annotation2214573/range2214573/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1655/annotation2214573/range2214573/1
type: Range
persistent identity: range2214573
display id: range2214573
version: 1
top level: BBa_M1655
direction: direction/forward
start: 4
end: 1296
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2214572
display id: annotation2214572
version: 1
top level: BBa_M1655
location: https://synbiohub.org/public/igem/BBa_M1655/annotation2214572/range2214572/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1655/annotation2214572/range2214572/1
type: Range
persistent identity: range2214572
display id: range2214572
version: 1
top level: BBa_M1655
direction: direction/forward
start: 1297
end: 1299
orientation: inline

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

https://synbiohub.org/public/igem/BBa_M1660_sequence/1
type: Sequence
persistent identity: BBa M1660 sequence
display id: BBa_M1660_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagacaggacctactagagcatcatcaccatcaccaccatcatcaccattaataatactagatgggggccatgacccaattactggccggtgttttcctggcgtttctggctctggcgacagaaggtggtgtactgaaaaaagtgattcgccacaagcggcaaagcggagtaaatgcgacgcttccggaagagaatcagccggttgtgttcaaccatgtttataacatcaaactgccggtgggttcccagtgttctgttgatttggagagtgcgagtggagaaaaggacctagcgccgccttcagagccgtcggagtctttccaggaacatacagttgatggtgaaaatcaaattgtgtttacccaccgtatcaatatcccgcgtcgcgcctgcggttgtgctgccgccccagacgttaaagagcttctgtcacgacttgaagaactggagaacttagtgagcagtctgcgtgaacagtgcacggcgggtgccggctgctgcctccagccggcaacaggtcgcctggacacccgccccttctgctcggggcgaggcaattttagcacggaaggttgcggctgcgtatgtgaacctgggtggaaaggtcctaattgttctgaaccagaatgccctgggaattgtcatctgcgcgggcgctgcattgacggccagtgcatttgtgacgatggttttactggcgaggactgttcacaattggcctgcccatcagactgcaatgatcaaggtaagtgcgtgaatggcgtatgcatttgcttcgaaggttatgcgggcgctgactgctctcgtgaaatctgtccagtaccctgctccgaagaacatggcacttgtgtggacgggttatgtgtatgtcacgacggatttgccggcgatgattgtaataaacctctttgtctgaacaactgttataatcgcggtcgctgtgtagaaaatgaatgcgtatgtgatgaaggatttaccggtgaggattgcagtgaactgatttgtcctaacgattgctttgaccggggacgatgcatcaacggcacctgctactgtgaggagggcttcaccggtgaggactgcggtaaaccgacctgtccgcacgcttgtcacacccaaggtcgctgcgaggagggccagtgtgtctgtgatgaaggcttcgccggcgttgattgctccgaaaagcgctgcccagcggattgccataaccgggggcgttgcgttgatggtcggtgcgagtgcgacgacggtttcaccggagcggattgtggcgaactgaaatgcccgaacggctgctctggccacggccgttgtgtgaatggccagtgtgtttgcgatgagggctataccggcgaagattgttctcaactgcgttgccccaatgactgccatagccgtgggcgctgtgtcgaaggaaagtgtgtgtgtgagcaaggctttaaaggttatgattgctcagatatgagctgtcctaatgactgtcatcagcacgggcgctgtgtaaatggtatgtgcgtttgtgatgatggctacaccggagaggattgccgcgatcgtcaatgtccgcgtgactgttctaatcgcggtctgtgtgtggatggacaatgtgtgtgcgaagacggcttcacgggaccggattgtgcggaactctcttgcccaaacgactgtcacggtcaaggtcgctgtgtgaacggtcaatgcgtgtgtcatgaaggttttatgggcaaggattgtaaggaacagcgatgcccttccgattgccacggtcaaggacgttgtgtagatggccagtgcatctgccatgaaggattcaccggtctcgattgcggtcagcactcctgcccgagcgactgtaacaacctcggccaatgtgtaagtggtcgctgcatttgcaatgaaggttactcaggggaagactgtagcgaagttagcccgccgaaggat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61101_sequence/1
type: Sequence
persistent identity: BBa J61101 sequence
display id: BBa_J61101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagacaggacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844000_sequence/1
type: Sequence
persistent identity: BBa K844000 sequence
display id: BBa_K844000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcaccatcaccaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1653_sequence/1
type: Sequence
persistent identity: BBa M1653 sequence
display id: BBa_M1653_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggggccatgacccaattactggccggtgttttcctggcgtttctggctctggcgacagaaggtggtgtactgaaaaaagtgattcgccacaagcggcaaagcggagtaaatgcgacgcttccggaagagaatcagccggttgtgttcaaccatgtttataacatcaaactgccggtgggttcccagtgttctgttgatttggagagtgcgagtggagaaaaggacctagcgccgccttcagagccgtcggagtctttccaggaacatacagttgatggtgaaaatcaaattgtgtttacccaccgtatcaatatcccgcgtcgcgcctgcggttgtgctgccgccccagacgttaaagagcttctgtcacgacttgaagaactggagaacttagtgagcagtctgcgtgaacagtgcacggcgggtgccggctgctgcctccagccggcaacaggtcgcctggacacccgccccttctgctcggggcgaggcaattttagcacggaaggttgcggctgcgtatgtgaacctgggtggaaaggtcctaattgttctgaaccagaatgccctgggaattgtcatctgcgcgggcgctgcattgacggccagtgcatttgtgacgatggttttactggcgaggactgttcacaattggcctgcccatcagactgcaatgatcaaggtaagtgcgtgaatggcgtatgcatttgcttcgaaggttatgcgggcgctgactgctctcgtgaaatctgtccagtaccctgctccgaagaacatggcacttgtgtggacgggttatgtgtatgtcacgacggatttgccggcgatgattgtaataaacctctttgtctgaacaactgttataatcgcggtcgctgtgtagaaaatgaatgcgtatgtgatgaaggatttaccggtgaggattgcagtgaactgatttgtcctaacgattgctttgaccggggacgatgcatcaacggcacctgctactgtgaggagggcttcaccggtgaggactgcggtaaaccgacctgtccgcacgcttgtcacacccaaggtcgctgcgaggagggccagtgtgtctgtgatgaaggcttcgccggcgttgattgctccgaaaagcgctgcccagcggattgccataaccgggggcgttgcgttgatggtcggtgcgagtgcgacgacggtttcaccggagcggattgtggcgaactgaaatgcccgaacggctgctctggccacggccgttgtgtgaatggccagtgtgtttgcgatgagggctataccggcgaagattgttctcaactgcgttgccccaatgactgccatagccgtgggcgctgtgtcgaaggaaagtgtgtgtgtgagcaaggctttaaaggttatgattgctcagatatgagctgtcctaatgactgtcatcagcacgggcgctgtgtaaatggtatgtgcgtttgtgatgatggctacaccggagaggattgccgcgatcgtcaatgtccgcgtgactgttctaatcgcggtctgtgtgtggatggacaatgtgtgtgcgaagacggcttcacgggaccggattgtgcggaactctcttgcccaaacgactgtcacggtcaaggtcgctgtgtgaacggtcaatgcgtgtgtcatgaaggttttatgggcaaggattgtaaggaacagcgatgcccttccgattgccacggtcaaggacgttgtgtagatggccagtgcatctgccatgaaggattcaccggtctcgattgcggtcagcactcctgcccgagcgactgtaacaacctcggccaatgtgtaagtggtcgctgcatttgcaatgaaggttactcaggggaagactgtagcgaagttagcccgccgaaggatttggtggttacagaagtcaccgaggaaacggttaatctggcctgggacaatgaaatgcgggtaaccgaatatctggtagtttatacacccacccatgagggcgggctggaaatgca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1655_sequence/1
type: Sequence
persistent identity: BBa M1655 sequence
display id: BBa_M1655_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccactagcatccgtcaatttacctcgagcagcagtataaaaggctccagcggcttgggaggcggatcaagccgcacaagctgtcgtctgagcggtggcctgggagctggctcatgccgtttaggttctgccggtggtttgggtagcacgctgggcggttcctcgtacagtagctgttacagctttggtagcggcggcggctacggatcaagcttcggcggggtcgatggcctgctcgctggcggagaaaaagccacgatgcagaacttaaacgaccgactcgccagctatctggataaggttcgtgcactggaagaagcaaatactgaactggaagttaaaatccgcgattggtatcagcgccaggcgccgggcccagcgcgggattacagccagtattatcgaaccattgaagagctccaaaacaaaattctgactgccacagttgataatgcgaatattcttttacagattgataatgcacgtctggcagctgacgactttcgcaccaaattcgagaccgaacaggcactccgtctgagtgtggaggctgatatcaacgggcttcgacgcgtgttggacgagttaaccttagcgcgcgcagacctagaaatgcagattgaaaacctgaaggaggagctcgcatatctcaaaaaaaatcatgaagaagaaatgaatgcgttgcgcggccaggtcggcggggaaatcaatgttgagatggatgctgccccgggtgtggatctcagtcgcattttaaatgaaatgcgtgatcagtatgagaaaatggcagaaaaaaaccgtaaagacgcggaagattggtttttctctaaaaccgaagaattgaaccgcgaagtcgcaaccaactctgaactggttcagtcggggaaaagcgaaatttccgaattacgtcgtacgatgcaggccctggaaatcgaactacaatcgcagctgtcaatgaaggccagcctcgaaggcaatttggccgaaaccgagaaccgctactgtgtacagctgtcccagatccaaggtctgattggttcagtcgaagagcaactggcgcagctcagatgcgaaatggaacaacagaaccaggaatataaaatcttacttgatgtgaaaacgcgcctggagcaggaaatcgcgacgtatcgtcgcttactggaaggggaagacgcccatttgacccagtacaaaaaggaaccagtgaccactcgccaagttcgcaccatcgtggaagaggtccaggacgggaaagttattagtagtcgcgaacaagtgcatcagaccacccgctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61103_sequence/1
type: Sequence
persistent identity: BBa J61103 sequence
display id: BBa_J61103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagattagaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z