LOCUS       BBa_M1676               1853 bp    DNA     linear       22-Dec-2024
DEFINITION  a construct to detect the presence/absence of glucose
ACCESSION   BBa_M1676
VERSION     BBa_M1676.1  
FEATURES             Location/Qualifiers
     misc_feature    10..24
                     /label= CAP binding site
     promoter        43..72
                     /label=lac promoter
     promoter        1..72
                     /label=BBa_M1674
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..773
                     /label=mrfp1
     misc_feature    780..804
                     /label=Help:Barcodes
     CDS             99..804
                     /label=BBa_E1010
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     terminator      813..941
                     /label=BBa_B0015
     misc_feature    1..941
                     /label=BBa_M1677
     promoter        950..970
                     /label=BBa_M1673
     misc_feature    983..986
                     /label=conserved
     RBS             979..990
                     /label=BBa_B0034
     CDS             997..1716
                     /label=GFP protein
     CDS             997..1716
                     /label=BBa_E0040
     stem_loop       1736..1779
                     /label=stem_loop
     misc_feature    1725..1804
                     /label=BBa_B0010
     terminator      1725..1804
                     /label=BBa_B0010
     stem_loop       1820..1839
                     /label=T7 TE
     misc_feature    1846..1846
                     /label=stop
     polyA_site      1840..1853
                     /label=polya
     misc_feature    1813..1853
                     /label=BBa_B0012
     terminator      1813..1853
                     /label=BBa_B0012
     terminator      1725..1853
                     /label=BBa_B0015
     misc_feature    950..1853
                     /label=BBa_M1690
ORIGIN
        1 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
       61 ggctcgtatg tttactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
      121 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      181 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      241 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
      301 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
      361 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
      421 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
      481 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
      541 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
      601 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
      661 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
      721 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
      781 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg ccagttctca
      961 cgtggtggcc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1021 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1081 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1141 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1201 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1261 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1321 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1381 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1441 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1501 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1561 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1621 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1681 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     1741 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1801 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//