a construct to detect the presence/absence of glucose
this construct can detect abnormal high/low glucose level by turning green/red colors.
Glucose detection using S14 gene [Sam Hawkins]
The rat S14 gene has been studied in Human hepatic (liver) cells for the detection of change in glucose concentration. The design is based on cloning this gene into E. coli based vector containing the GFP expression gene.
a biobrick construct to detect the absence of glucose
This device uses RFP as the reporter to detect the absence of glucose by using a cAMP responsive Promoter.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Double terminator consisting of BBa_B0010 and BBa_B0012
S14 promoter is a 21-bp segment (-1448/-1428) of the S14 gene of rats and is stimulated in response to increased concentration of glucose.
This promoter is segment copied from The Lac Operon which contains the catabolite activator protein (CAP) binding site and the Lac promoter. Low glucose concentration results in increased adenylate cyclase activity, which up-regulate the cAMP level. cAMP is able to bind to CAP and activate transcription of downstream gene.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1