LOCUS       BBa_M1677                941 bp    DNA     linear       22-Dec-2024
DEFINITION  a biobrick construct to detect the absence of glucose
ACCESSION   BBa_M1677
VERSION     BBa_M1677.1  
FEATURES             Location/Qualifiers
     misc_feature    10..24
                     /label= CAP binding site
     promoter        43..72
                     /label=lac promoter
     promoter        1..72
                     /label=BBa_M1674
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..773
                     /label=mrfp1
     misc_feature    780..804
                     /label=Help:Barcodes
     CDS             99..804
                     /label=BBa_E1010
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     terminator      813..941
                     /label=BBa_B0015
ORIGIN
        1 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
       61 ggctcgtatg tttactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
      121 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      181 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      241 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
      301 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
      361 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
      421 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
      481 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
      541 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
      601 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
      661 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
      721 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
      781 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//