LOCUS       BBa_M1690                904 bp    DNA     linear       22-Nov-2024
DEFINITION  Glucose detection using S14 gene [Sam Hawkins]
ACCESSION   BBa_M1690
VERSION     BBa_M1690.1  
FEATURES             Location/Qualifiers
     promoter        1..21
                     /label=BBa_M1673
     misc_feature    34..37
                     /label=conserved
     RBS             30..41
                     /label=BBa_B0034
     CDS             48..767
                     /label=GFP protein
     CDS             48..767
                     /label=BBa_E0040
     stem_loop       787..830
                     /label=stem_loop
     misc_feature    776..855
                     /label=BBa_B0010
     terminator      776..855
                     /label=BBa_B0010
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     terminator      776..904
                     /label=BBa_B0015
ORIGIN
        1 gccagttctc acgtggtggc ctactagaga aagaggagaa atactagatg cgtaaaggag
       61 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      121 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      181 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      241 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      301 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      361 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      421 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      481 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      541 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      601 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      661 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      721 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tata
//
LOCUS       BBa_M1678                904 bp    DNA     linear       22-Nov-2024
DEFINITION  a construct to detect the presence/absence of glucose
ACCESSION   BBa_M1678
VERSION     BBa_M1678.1  
FEATURES             Location/Qualifiers
     promoter        1..21
                     /label=BBa_M1673
     misc_feature    34..37
                     /label=conserved
     RBS             30..41
                     /label=BBa_B0034
     CDS             48..767
                     /label=GFP protein
     CDS             48..767
                     /label=BBa_E0040
     stem_loop       787..830
                     /label=stem_loop
     misc_feature    776..855
                     /label=BBa_B0010
     terminator      776..855
                     /label=BBa_B0010
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     terminator      776..904
                     /label=BBa_B0015
ORIGIN
        1 gccagttctc acgtggtggc ctactagaga aagaggagaa atactagatg cgtaaaggag
       61 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      121 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      181 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      241 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      301 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      361 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      421 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      481 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      541 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      601 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      661 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      721 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tata
//