BBa_M1699 1 BBa_M1699 scrT 2013-04-14T11:00:00Z 2015-05-08T01:13:58Z http://www.ncbi.nlm.nih.gov/protein/22550282 This gene sequence codes for the ScrT protein which serves as a symporter for sucrose into the cell. The sequence is originally from [Bifidobacterium animalis subsp. lactis]. The sequence is normally a part of a gene cluster containing scrT, scrP, and scrR. Only scrT was used specifically from these gene cluster in our project to increase the uptake of sucrose by the cell. The sequence present in this part has been optimized for use in Lactobacillus johnsonii. false false _768_ 0 16137 9 Not in stock false This sequence was put into DNA 2.0's Gene Designer software to optimize its G-C content for use in Lactobacillus Johnsonii. Also it was checked for comparability with standard 10 restriction enzymes. The sequence here does not contain any restriction site conflicts with standard 10. false S. Ivan Bartlett BBa_M1701 1 BBa_M1701 ScrR regulatory promoter and a RBS 2013-04-14T11:00:00Z 2015-05-08T01:13:58Z This is a combination of part M1696 and M1694 This is the promoter to which binds ScrR to regulate the transcription of a gene. We also added onto the end a RBS from Lactobacillus Sakei as identified on the NCBI website. false false _768_ 0 16135 9 Not in stock false We needed a promoter that ScrR regulates and a RBS that works in Lactobacillus, so we had to seek those out. false Benjamin L Hanks component2215033 1 BBa_M1694 component2215032 1 BBa_M1697 annotation2215033 1 BBa_M1694 range2215033 1 88 101 annotation2215032 1 BBa_M1697 range2215032 1 1 79 BBa_M1703 1 BBa_M1703 Inulosucrase and scrT sucrose transport protein composite part 2013-04-17T11:00:00Z 2015-05-08T01:13:58Z composite part inulosucrase enzyme and sucrose transporter scrT downstream of sucrose regulated promoter false false _768_ 0 16139 9 Not in stock false codon optimization false Jonathan L. Wood component2215282 1 BBa_M1698 component2215285 1 BBa_M1700 component2215281 1 BBa_M1701 component2215283 1 BBa_M1694 component2215284 1 BBa_M1699 annotation2215284 1 BBa_M1699 range2215284 1 2527 3828 annotation2215283 1 BBa_M1694 range2215283 1 2507 2520 annotation2215285 1 BBa_M1700 range2215285 1 3837 3866 annotation2215281 1 BBa_M1701 range2215281 1 1 101 annotation2215282 1 BBa_M1698 range2215282 1 108 2498 BBa_M1694 1 BBa_M1694 Ribosome binding site 2013-04-09T11:00:00Z 2015-05-08T01:13:57Z Ribosome binding site for ScrR in Lactobacillus sakei subsp. sakei as identified in NCBI gene database. This ribosome binding site initiates translation for the bacteria Lactobacillus. false false _768_ 0 16138 9 Not in stock false It needed to initiate for Lactobacillus. false Tiffany Hansen BBa_M1700 1 BBa_M1700 L. Johnsonii transcription terminator 2013-04-14T11:00:00Z 2015-05-08T01:13:58Z From L. johnsonii derived by use of the Webgester DB. http://pallab.serc.iisc.ernet.in/gester/65481116/index.html A transcription terminator native to L. johnsonii NCC 533. This terminator originally exists downstream of the rpsR coding sequence. It was used in our project downstream from scrT to terminate transcription. This terminator has a deltaG of -12.33. false false _768_ 0 16137 9 Not in stock false This sequence was not altered in any way since it was to be used in L. johnsonii and that is the native organism from which it was derived. false S. Ivan Bartlett BBa_M1698 1 BBa_M1698 Inulosucrase 2013-04-14T11:00:00Z 2015-05-08T01:13:58Z Lactobacillus johnsonil NCC 533 This enzyme can degrade the fructose into inulin, which can not be taken into human body. false false _768_ 0 16136 9 Not in stock false Optimized this enzyme in DNA 2.0 for compatibility with E. coli. false Qifei Li BBa_M1697 1 BBa_M1697 Sucrose Mediated Regulatory Promoter 2013-04-10T11:00:00Z 2015-05-08T01:13:58Z (Br??ckner, 1996) Sucrose mediated promoter false false _768_ 0 16139 9 Not in stock false contains operator site for sucrose mediated repressor false Jonathan L. Wood annotation2214848 1 operator range2214848 1 40 56 annotation2214847 1 promoter range2214847 1 1 79 BBa_M1700_sequence 1 tctcatcgaatacgatgagaaaaataaaac BBa_M1701_sequence 1 ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaattactagagttcagggggggaaa BBa_M1703_sequence 1 ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaattactagagttcagggggggaaatactagatgttggagaacaagaaccacaagaaaataagcctgagcgggaaatcactgctgatgggaacattatccacggcggcgattgtcctttccgcgagcaccgctaacgccgctaccataaacgcggacaatgtaaatgagaatcaaacggttgaagtaacagcatcttccgtaaataatgaaaacaacaagcaagtgaccgaaaaagatagcgctgacaagtctacctctgatgtagcggaggacgcgaataccaaaaaatccaatgagaacacggaaacgacagaaaagaatacccaaacagtcgttacaaacgcgccggtgtctgatgtgaagaatacgaatactgttaccgctgagactccggttgataaggttgtcaataattccgatcagaaaaccaccaacgctgcaaccacggatactaagaaagacgatgtaaaacaggtagaaaaaaaagattcggttgacaaaactaatgcagaagaaaataaagatagtagcgtaaaacccgctgaaaatgctacgaaagcagagttaaaaggtcaggtcaaagatatagttgaggaatcgggggtggatacgtctaaactaacaaacgatcagatcaacgaactgaacaaaatcaatttctctaaagaagccaaaagcggcacacagttaacatataatgattttaagaagatcgctaaaaccttgatcgagcaggatgcaaggtatgctattccattttttaatgccagcaagattaaaaacatgcccgctgccaaaaccctggatgcgcaatcaggtaaagttgaagacctggagatttgggacagttggccggtgcaagacgccaagacgggttacgtttctaattggaacggctatcaattggtaattggcatgatgggagttccgaatgttaacgataatcatatttatcttttatacaataaatatggcgataacgacttcaatcattggaaaaatgcagggccgatctttggtttaggcaccccagtaattcaacagtggtccggctcggcgactttgaataaagatggttcaattcaactgtactatactaaagtggatacatccgataacaacacgaatcatcagaaactggcgtccgcgaccgtgtatctgaatctggaaaaagatcaggataagatttcaatagcacatgtagacaatgaccacattgtttttgaaggtgatggctatcattaccagacctatgatcagtggaaagagactaacaaaggtgctgacaatattgcgatgagagatgcccacgtaattgacgatgacaatggcaataggtacctcgtattcgaggcctccacgggtacagagaattaccaaggtgacgatcagatctaccagtggttaaattacggtggtacgaataaagataacctgggggattttttccaaatcttaagcaacagcgatattaaagacagagctaaatggagtaatgcggcgatagggatcatcaaattaaatgatgatgtgaaaaacccgtccgttgcaaaggtatactcgccgttaatctcggctccgatggtgtctgacgagatagaacgtcccgatgtggttaaactgggcaataaatactatctctttgcggctactagactcaatcgagggtcgaatgacgacgcttggatggccacgaataaagccgtgggcgataatgtcgcaatgattggttacgtaagcgacaacctgacacatggatatgttcctctcaatgagtctggtgttgtgttgacggcttctgttccagctaactggcgtacggccacatattcgtactatgccgttcccgtggaagggcgtgacgatcaactgctgattacctcatatatcactaatcgcggcgaagttgcaggaaaggggatgcacgccacatgggctccctcttttctgctacaaatcaatccggataatacaacaacggtactggccaagatgacgaatcagggagattggatttgggacgattcatcggaaaatcctgacatgatgggcgtacttgagaaggatgctcctaattcggcggcgttacctggcgaatggggtaaaccggttgattgggatctgatcggtgggtacaatttaaagccccatcagccagtcacccccattcctaacgtaccaactacgccggagaccccgaccacgccagataaaccagaagtacccactacccccgaagttccgactacacccgagacccctactcctgaagcccctaaaaatccggtgaaaaaaacaagccagagcaaacttccgaaagcgggcgataaaaatagttttgccgctgttgtgctgggggccgtgtcatctattcttggcgctgttgggttaacaggggtgtcgaaacgcaaaagaaataattactagagttcagggggggaaatactagatggtctctgctgatggtcatcgtccgaccaaagcagaattagtccgcttaggaattggcttcactgtgtctgcggtggcctgtgctataccctgggtggccctttccacggttattctgccacgcgtttttgaacttatcgataattccagcaaagaagcgatgcttgggttggtcaactcgatcggtgctatcgttgccctgctcgcaaacatcatttttggtactctgagtgatatgactcgttcccgctttggaaaacgtaccccctggatcgtgggtggcggaattatcacgggtttagccattggcgctatagcgtttacccgtaacgaagccttgattatctttctctggtgcgtggcccagatgggatataacatgatgctggcaccgtatgttgcagtgatgtcggatcgtgtgcctgataaagttcgaggaactatatctggattctacggggccggtattgccgtcggacagactctgggatccgttgttggggccgctttcctgcgacaggggcaaagtggtctgttttctgcctggatgatgggtctgggcatattctctctaacaggtattttcgtggtggctatgtggccgcgtgagaagagttctcgcttcgaagaacagtcagaacatgtaaccgtaaagagtgtacttctgaactttcgtcctcctcgtcatgctccagatttttattacgcacttgttggtaggacactgatgatgggaggttattggatgatcaccacgtaccagctgtatatagcccaggactatatttatgccggagaccccaatgcaaaagacaaagcggcgactattattgctacgatgggtgttattaccttggtggtttcccttatcgcagcagtgactgccggacccataactgatagactgggccgcagaaagctgccagttgcgttagccagttgcctctttgcggtaggtgccgcaatgcccctgttttttcggagccctatgggtatgtatctctttgcagcagtcgccggtttaggctacggtgtctataatgcaatagatcaagcgttaaatgttgctgttctccctaatcctaaagaagcggggaaggatttaggcattctgaatctggcaaataccctttctacggtgattggcaccgcaatgacttctctagttgtttctattgtgaaatcaagcatgcacgtctctactacccctgcggtcgcgtacgcttgggtctttggcgtggccatcgtgattgtcctggccgctgctgcgctgattatgcgcattcgaaacgtgaaataatactagagtctcatcgaatacgatgagaaaaataaaac BBa_M1694_sequence 1 ttcagggggggaaa BBa_M1697_sequence 1 ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaat BBa_M1699_sequence 1 atggtctctgctgatggtcatcgtccgaccaaagcagaattagtccgcttaggaattggcttcactgtgtctgcggtggcctgtgctataccctgggtggccctttccacggttattctgccacgcgtttttgaacttatcgataattccagcaaagaagcgatgcttgggttggtcaactcgatcggtgctatcgttgccctgctcgcaaacatcatttttggtactctgagtgatatgactcgttcccgctttggaaaacgtaccccctggatcgtgggtggcggaattatcacgggtttagccattggcgctatagcgtttacccgtaacgaagccttgattatctttctctggtgcgtggcccagatgggatataacatgatgctggcaccgtatgttgcagtgatgtcggatcgtgtgcctgataaagttcgaggaactatatctggattctacggggccggtattgccgtcggacagactctgggatccgttgttggggccgctttcctgcgacaggggcaaagtggtctgttttctgcctggatgatgggtctgggcatattctctctaacaggtattttcgtggtggctatgtggccgcgtgagaagagttctcgcttcgaagaacagtcagaacatgtaaccgtaaagagtgtacttctgaactttcgtcctcctcgtcatgctccagatttttattacgcacttgttggtaggacactgatgatgggaggttattggatgatcaccacgtaccagctgtatatagcccaggactatatttatgccggagaccccaatgcaaaagacaaagcggcgactattattgctacgatgggtgttattaccttggtggtttcccttatcgcagcagtgactgccggacccataactgatagactgggccgcagaaagctgccagttgcgttagccagttgcctctttgcggtaggtgccgcaatgcccctgttttttcggagccctatgggtatgtatctctttgcagcagtcgccggtttaggctacggtgtctataatgcaatagatcaagcgttaaatgttgctgttctccctaatcctaaagaagcggggaaggatttaggcattctgaatctggcaaataccctttctacggtgattggcaccgcaatgacttctctagttgtttctattgtgaaatcaagcatgcacgtctctactacccctgcggtcgcgtacgcttgggtctttggcgtggccatcgtgattgtcctggccgctgctgcgctgattatgcgcattcgaaacgtgaaataa BBa_M1698_sequence 1 atgttggagaacaagaaccacaagaaaataagcctgagcgggaaatcactgctgatgggaacattatccacggcggcgattgtcctttccgcgagcaccgctaacgccgctaccataaacgcggacaatgtaaatgagaatcaaacggttgaagtaacagcatcttccgtaaataatgaaaacaacaagcaagtgaccgaaaaagatagcgctgacaagtctacctctgatgtagcggaggacgcgaataccaaaaaatccaatgagaacacggaaacgacagaaaagaatacccaaacagtcgttacaaacgcgccggtgtctgatgtgaagaatacgaatactgttaccgctgagactccggttgataaggttgtcaataattccgatcagaaaaccaccaacgctgcaaccacggatactaagaaagacgatgtaaaacaggtagaaaaaaaagattcggttgacaaaactaatgcagaagaaaataaagatagtagcgtaaaacccgctgaaaatgctacgaaagcagagttaaaaggtcaggtcaaagatatagttgaggaatcgggggtggatacgtctaaactaacaaacgatcagatcaacgaactgaacaaaatcaatttctctaaagaagccaaaagcggcacacagttaacatataatgattttaagaagatcgctaaaaccttgatcgagcaggatgcaaggtatgctattccattttttaatgccagcaagattaaaaacatgcccgctgccaaaaccctggatgcgcaatcaggtaaagttgaagacctggagatttgggacagttggccggtgcaagacgccaagacgggttacgtttctaattggaacggctatcaattggtaattggcatgatgggagttccgaatgttaacgataatcatatttatcttttatacaataaatatggcgataacgacttcaatcattggaaaaatgcagggccgatctttggtttaggcaccccagtaattcaacagtggtccggctcggcgactttgaataaagatggttcaattcaactgtactatactaaagtggatacatccgataacaacacgaatcatcagaaactggcgtccgcgaccgtgtatctgaatctggaaaaagatcaggataagatttcaatagcacatgtagacaatgaccacattgtttttgaaggtgatggctatcattaccagacctatgatcagtggaaagagactaacaaaggtgctgacaatattgcgatgagagatgcccacgtaattgacgatgacaatggcaataggtacctcgtattcgaggcctccacgggtacagagaattaccaaggtgacgatcagatctaccagtggttaaattacggtggtacgaataaagataacctgggggattttttccaaatcttaagcaacagcgatattaaagacagagctaaatggagtaatgcggcgatagggatcatcaaattaaatgatgatgtgaaaaacccgtccgttgcaaaggtatactcgccgttaatctcggctccgatggtgtctgacgagatagaacgtcccgatgtggttaaactgggcaataaatactatctctttgcggctactagactcaatcgagggtcgaatgacgacgcttggatggccacgaataaagccgtgggcgataatgtcgcaatgattggttacgtaagcgacaacctgacacatggatatgttcctctcaatgagtctggtgttgtgttgacggcttctgttccagctaactggcgtacggccacatattcgtactatgccgttcccgtggaagggcgtgacgatcaactgctgattacctcatatatcactaatcgcggcgaagttgcaggaaaggggatgcacgccacatgggctccctcttttctgctacaaatcaatccggataatacaacaacggtactggccaagatgacgaatcagggagattggatttgggacgattcatcggaaaatcctgacatgatgggcgtacttgagaaggatgctcctaattcggcggcgttacctggcgaatggggtaaaccggttgattgggatctgatcggtgggtacaatttaaagccccatcagccagtcacccccattcctaacgtaccaactacgccggagaccccgaccacgccagataaaccagaagtacccactacccccgaagttccgactacacccgagacccctactcctgaagcccctaaaaatccggtgaaaaaaacaagccagagcaaacttccgaaagcgggcgataaaaatagttttgccgctgttgtgctgggggccgtgtcatctattcttggcgctgttgggttaacaggggtgtcgaaacgcaaaagaaataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z