BBa_M1705
type: ComponentDefinition
persistent identity: BBa M1705
display id: BBa_M1705
version: 1
was generated by: iGEM to SBOL conversion
description: Inulosucrase/scrR/scrT complete operon
created: 2013-04-18T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: This is a composite part built from part M1704 (promoter+RBS+scrR+terminator) and part M1703 (promoter+RBS+inulosucrase+RBS+scrT+terminator). With this combination it is now a complete operon for the increased uptake of sucrose into the cell and conversion of sucrose to inulin.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: S. Ivan Bartlett
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1703
component: BBa_M1704
sequence annotation: BBa_M1704
sequence annotation: BBa_M1703
sequence: https://synbiohub.org/public/igem/BBa_M1705_sequence/1

BBa_M1703
type: Component
persistent identity: component2215308
display id: component2215308
version: 1
top level: BBa_M1705
definition: BBa_M1703
access: public

BBa_M1704
type: Component
persistent identity: component2215298
display id: component2215298
version: 1
top level: BBa_M1705
definition: BBa_M1704
access: public

BBa_M1704
type: SequenceAnnotation
persistent identity: annotation2215298
display id: annotation2215298
version: 1
top level: BBa_M1705
location: https://synbiohub.org/public/igem/BBa_M1705/annotation2215298/range2215298/1
component: BBa_M1704

https://synbiohub.org/public/igem/BBa_M1705/annotation2215298/range2215298/1
type: Range
persistent identity: range2215298
display id: range2215298
version: 1
top level: BBa_M1705
direction: direction/reverse
start: 1
end: 1084
orientation: inline

BBa_M1703
type: SequenceAnnotation
persistent identity: annotation2215308
display id: annotation2215308
version: 1
top level: BBa_M1705
location: https://synbiohub.org/public/igem/BBa_M1705/annotation2215308/range2215308/1
component: BBa_M1703

https://synbiohub.org/public/igem/BBa_M1705/annotation2215308/range2215308/1
type: Range
persistent identity: range2215308
display id: range2215308
version: 1
top level: BBa_M1705
direction: direction/reverse
start: 1093
end: 4958
orientation: inline

BBa_M1703
type: ComponentDefinition
persistent identity: BBa M1703
display id: BBa_M1703
version: 1
was generated by: iGEM to SBOL conversion
description: Inulosucrase and scrT sucrose transport protein composite part
created: 2013-04-17T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: composite part
owned by: james
owned by: myers
mutable description: inulosucrase enzyme and sucrose transporter scrT downstream of sucrose regulated promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16139
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: codon optimization
star: false
creator: Jonathan L. Wood
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1701
component: BBa_M1700
component: BBa_M1699
component: BBa_M1694
component: BBa_M1698
sequence annotation: BBa_M1701
sequence annotation: BBa_M1698
sequence annotation: BBa_M1694
sequence annotation: BBa_M1699
sequence annotation: BBa_M1700
sequence: https://synbiohub.org/public/igem/BBa_M1703_sequence/1

BBa_M1701
type: Component
persistent identity: component2215281
display id: component2215281
version: 1
top level: BBa_M1703
definition: BBa_M1701
access: public

BBa_M1700
type: Component
persistent identity: component2215285
display id: component2215285
version: 1
top level: BBa_M1703
definition: BBa_M1700
access: public

BBa_M1699
type: Component
persistent identity: component2215284
display id: component2215284
version: 1
top level: BBa_M1703
definition: BBa_M1699
access: public

BBa_M1694
type: Component
persistent identity: component2215283
display id: component2215283
version: 1
top level: BBa_M1703
definition: BBa_M1694
access: public

BBa_M1698
type: Component
persistent identity: component2215282
display id: component2215282
version: 1
top level: BBa_M1703
definition: BBa_M1698
access: public

BBa_M1701
type: SequenceAnnotation
persistent identity: annotation2215281
display id: annotation2215281
version: 1
top level: BBa_M1703
location: https://synbiohub.org/public/igem/BBa_M1703/annotation2215281/range2215281/1
component: BBa_M1701

https://synbiohub.org/public/igem/BBa_M1703/annotation2215281/range2215281/1
type: Range
persistent identity: range2215281
display id: range2215281
version: 1
top level: BBa_M1703
direction: direction/reverse
start: 1
end: 101
orientation: inline

BBa_M1698
type: SequenceAnnotation
persistent identity: annotation2215282
display id: annotation2215282
version: 1
top level: BBa_M1703
location: https://synbiohub.org/public/igem/BBa_M1703/annotation2215282/range2215282/1
component: BBa_M1698

https://synbiohub.org/public/igem/BBa_M1703/annotation2215282/range2215282/1
type: Range
persistent identity: range2215282
display id: range2215282
version: 1
top level: BBa_M1703
direction: direction/reverse
start: 108
end: 2498
orientation: inline

BBa_M1694
type: SequenceAnnotation
persistent identity: annotation2215283
display id: annotation2215283
version: 1
top level: BBa_M1703
location: https://synbiohub.org/public/igem/BBa_M1703/annotation2215283/range2215283/1
component: BBa_M1694

https://synbiohub.org/public/igem/BBa_M1703/annotation2215283/range2215283/1
type: Range
persistent identity: range2215283
display id: range2215283
version: 1
top level: BBa_M1703
direction: direction/reverse
start: 2507
end: 2520
orientation: inline

BBa_M1699
type: SequenceAnnotation
persistent identity: annotation2215284
display id: annotation2215284
version: 1
top level: BBa_M1703
location: https://synbiohub.org/public/igem/BBa_M1703/annotation2215284/range2215284/1
component: BBa_M1699

https://synbiohub.org/public/igem/BBa_M1703/annotation2215284/range2215284/1
type: Range
persistent identity: range2215284
display id: range2215284
version: 1
top level: BBa_M1703
direction: direction/reverse
start: 2527
end: 3828
orientation: inline

BBa_M1700
type: SequenceAnnotation
persistent identity: annotation2215285
display id: annotation2215285
version: 1
top level: BBa_M1703
location: https://synbiohub.org/public/igem/BBa_M1703/annotation2215285/range2215285/1
component: BBa_M1700

https://synbiohub.org/public/igem/BBa_M1703/annotation2215285/range2215285/1
type: Range
persistent identity: range2215285
display id: range2215285
version: 1
top level: BBa_M1703
direction: direction/reverse
start: 3837
end: 3866
orientation: inline

BBa_M1704
type: ComponentDefinition
persistent identity: BBa M1704
display id: BBa_M1704
version: 1
was generated by: iGEM to SBOL conversion
description: ScrR Promoter + RBS + ScrR +Transcription terminator
created: 2013-04-18T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Composite part
owned by: james
owned by: myers
mutable description: this is the composite part with promoter, RBS, ScrR and terminator for Inulosucrase to digest fructose into inulin, which will not be absorbed by human body.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16136
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BBa_M1695 is ScrR promoter BBa_M1694 RBS BBa_M1696 ScrR BBa_M1700 terminator.
star: false
creator: Qifei Li
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1700
component: BBa_M1696
component: BBa_M1694
component: BBa_M1695
sequence annotation: BBa_M1695
sequence annotation: BBa_M1694
sequence annotation: BBa_M1696
sequence annotation: BBa_M1700
sequence: https://synbiohub.org/public/igem/BBa_M1704_sequence/1

BBa_M1700
type: Component
persistent identity: component2215291
display id: component2215291
version: 1
top level: BBa_M1704
definition: BBa_M1700
access: public

BBa_M1696
type: Component
persistent identity: component2215290
display id: component2215290
version: 1
top level: BBa_M1704
definition: BBa_M1696
access: public

BBa_M1694
type: Component
persistent identity: component2215289
display id: component2215289
version: 1
top level: BBa_M1704
definition: BBa_M1694
access: public

BBa_M1695
type: Component
persistent identity: component2215288
display id: component2215288
version: 1
top level: BBa_M1704
definition: BBa_M1695
access: public

BBa_M1695
type: SequenceAnnotation
persistent identity: annotation2215288
display id: annotation2215288
version: 1
top level: BBa_M1704
location: https://synbiohub.org/public/igem/BBa_M1704/annotation2215288/range2215288/1
component: BBa_M1695

https://synbiohub.org/public/igem/BBa_M1704/annotation2215288/range2215288/1
type: Range
persistent identity: range2215288
display id: range2215288
version: 1
top level: BBa_M1704
direction: direction/reverse
start: 1
end: 31
orientation: inline

BBa_M1694
type: SequenceAnnotation
persistent identity: annotation2215289
display id: annotation2215289
version: 1
top level: BBa_M1704
location: https://synbiohub.org/public/igem/BBa_M1704/annotation2215289/range2215289/1
component: BBa_M1694

https://synbiohub.org/public/igem/BBa_M1704/annotation2215289/range2215289/1
type: Range
persistent identity: range2215289
display id: range2215289
version: 1
top level: BBa_M1704
direction: direction/reverse
start: 40
end: 53
orientation: inline

BBa_M1696
type: SequenceAnnotation
persistent identity: annotation2215290
display id: annotation2215290
version: 1
top level: BBa_M1704
location: https://synbiohub.org/public/igem/BBa_M1704/annotation2215290/range2215290/1
component: BBa_M1696

https://synbiohub.org/public/igem/BBa_M1704/annotation2215290/range2215290/1
type: Range
persistent identity: range2215290
display id: range2215290
version: 1
top level: BBa_M1704
direction: direction/reverse
start: 60
end: 1046
orientation: inline

BBa_M1700
type: SequenceAnnotation
persistent identity: annotation2215291
display id: annotation2215291
version: 1
top level: BBa_M1704
location: https://synbiohub.org/public/igem/BBa_M1704/annotation2215291/range2215291/1
component: BBa_M1700

https://synbiohub.org/public/igem/BBa_M1704/annotation2215291/range2215291/1
type: Range
persistent identity: range2215291
display id: range2215291
version: 1
top level: BBa_M1704
direction: direction/reverse
start: 1055
end: 1084
orientation: inline

BBa_M1696
type: ComponentDefinition
persistent identity: BBa M1696
display id: BBa_M1696
version: 1
was generated by: iGEM to SBOL conversion
description: scrR LacI family sucrose operon repressor
created: 2013-04-09T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: scrR sequence found in Lactobacillus sakei subsp. sakei as identified in NCBI gene database
owned by: james
owned by: myers
mutable description: ScrR is in the LacI family for operon repression. It negatively regulates by binding as a repressor to the promoter. In the presence of sucrose it is deactivated and transcription can occur.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16135
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Checked for RE sites. Also had to find how it is promoted because it appears to be immediatly downstream from the gene it regulates
star: false
creator: Benjamin L Hanks
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M1696_sequence/1

BBa_M1694
type: ComponentDefinition
persistent identity: BBa M1694
display id: BBa_M1694
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome binding site
created: 2013-04-09T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Ribosome binding site for ScrR in Lactobacillus sakei subsp. sakei as identified in NCBI gene database.
owned by: james
owned by: myers
mutable description: This ribosome binding site initiates translation for the bacteria Lactobacillus.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It needed to initiate for Lactobacillus.
star: false
creator: Tiffany Hansen
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M1694_sequence/1

BBa_M1695
type: ComponentDefinition
persistent identity: BBa M1695
display id: BBa_M1695
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter for ScrR
created: 2013-04-09T11:00:00Z
modified: 2015-05-08T01:13:57Z
mutable provenance: Promoter for ScrR in Lactobacillus agilis as identified in NCBI gene database.
owned by: james
owned by: myers
mutable description: This promoter is for the bacteria Lactobacillus agilis, it promotes for the protein ScrR at high efficiency.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Needed to work in Lactobacillus.
star: false
creator: Tiffany Hansen
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_M1695_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2215025
display id: annotation2215025
version: 1
top level: BBa_M1695
location: https://synbiohub.org/public/igem/BBa_M1695/annotation2215025/range2215025/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M1695/annotation2215025/range2215025/1
type: Range
persistent identity: range2215025
display id: range2215025
version: 1
top level: BBa_M1695
direction: direction/forward
start: 1
end: 31
orientation: inline

BBa_M1701
type: ComponentDefinition
persistent identity: BBa M1701
display id: BBa_M1701
version: 1
was generated by: iGEM to SBOL conversion
description: ScrR regulatory promoter and a RBS
created: 2013-04-14T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: This is a combination of part M1696 and M1694
owned by: james
owned by: myers
mutable description: This is the promoter to which binds ScrR to regulate the transcription of a gene. We also added onto the end a RBS from Lactobacillus Sakei as identified on the NCBI website.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16135
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We needed a promoter that ScrR regulates and a RBS that works in Lactobacillus, so we had to seek those out.
star: false
creator: Benjamin L Hanks
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1694
component: BBa_M1697
sequence annotation: BBa_M1697
sequence annotation: BBa_M1694
sequence: https://synbiohub.org/public/igem/BBa_M1701_sequence/1

BBa_M1694
type: Component
persistent identity: component2215033
display id: component2215033
version: 1
top level: BBa_M1701
definition: BBa_M1694
access: public

BBa_M1697
type: Component
persistent identity: component2215032
display id: component2215032
version: 1
top level: BBa_M1701
definition: BBa_M1697
access: public

BBa_M1697
type: SequenceAnnotation
persistent identity: annotation2215032
display id: annotation2215032
version: 1
top level: BBa_M1701
location: https://synbiohub.org/public/igem/BBa_M1701/annotation2215032/range2215032/1
component: BBa_M1697

https://synbiohub.org/public/igem/BBa_M1701/annotation2215032/range2215032/1
type: Range
persistent identity: range2215032
display id: range2215032
version: 1
top level: BBa_M1701
direction: direction/reverse
start: 1
end: 79
orientation: inline

BBa_M1694
type: SequenceAnnotation
persistent identity: annotation2215033
display id: annotation2215033
version: 1
top level: BBa_M1701
location: https://synbiohub.org/public/igem/BBa_M1701/annotation2215033/range2215033/1
component: BBa_M1694

https://synbiohub.org/public/igem/BBa_M1701/annotation2215033/range2215033/1
type: Range
persistent identity: range2215033
display id: range2215033
version: 1
top level: BBa_M1701
direction: direction/reverse
start: 88
end: 101
orientation: inline

BBa_M1698
type: ComponentDefinition
persistent identity: BBa M1698
display id: BBa_M1698
version: 1
was generated by: iGEM to SBOL conversion
description: Inulosucrase
created: 2013-04-14T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Lactobacillus johnsonil NCC 533
owned by: james
owned by: myers
mutable description: This enzyme can degrade the fructose into inulin, which can not be taken into human body.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16136
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Optimized this enzyme in DNA 2.0 for compatibility with E. coli.
star: false
creator: Qifei Li
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M1698_sequence/1

BBa_M1699
type: ComponentDefinition
persistent identity: BBa M1699
display id: BBa_M1699
version: 1
was generated by: iGEM to SBOL conversion
description: scrT
created: 2013-04-14T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/22550282
owned by: james
owned by: myers
mutable description: This gene sequence codes for the ScrT protein which serves as a symporter for sucrose into the cell. The sequence is originally from [Bifidobacterium animalis subsp. lactis]. The sequence is normally a part of a gene cluster containing scrT, scrP, and scrR. Only scrT was used specifically from these gene cluster in our project to increase the uptake of sucrose by the cell. The sequence present in this part has been optimized for use in Lactobacillus johnsonii.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was put into DNA 2.0's Gene Designer software to optimize its G-C content for use in Lactobacillus Johnsonii. Also it was checked for comparability with standard 10 restriction enzymes. The sequence here does not contain any restriction site conflicts with standard 10.
star: false
creator: S. Ivan Bartlett
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M1699_sequence/1

BBa_M1700
type: ComponentDefinition
persistent identity: BBa M1700
display id: BBa_M1700
version: 1
was generated by: iGEM to SBOL conversion
description: L. Johnsonii transcription terminator
created: 2013-04-14T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: From L. johnsonii derived by use of the Webgester DB. http://pallab.serc.iisc.ernet.in/gester/65481116/index.html
owned by: james
owned by: myers
mutable description: A transcription terminator native to L. johnsonii NCC 533. This terminator originally exists downstream of the rpsR coding sequence. It was used in our project downstream from scrT to terminate transcription. This terminator has a deltaG of -12.33.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was not altered in any way since it was to be used in L. johnsonii and that is the native organism from which it was derived.
star: false
creator: S. Ivan Bartlett
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_M1700_sequence/1

BBa_M1697
type: ComponentDefinition
persistent identity: BBa M1697
display id: BBa_M1697
version: 1
was generated by: iGEM to SBOL conversion
description: Sucrose Mediated Regulatory Promoter
created: 2013-04-10T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: (Br??ckner, 1996)
owned by: james
owned by: myers
mutable description: Sucrose mediated promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16139
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: contains operator site for sucrose mediated repressor
star: false
creator: Jonathan L. Wood
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: promoter
sequence annotation: operator
sequence: https://synbiohub.org/public/igem/BBa_M1697_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2214847
display id: annotation2214847
version: 1
top level: BBa_M1697
location: https://synbiohub.org/public/igem/BBa_M1697/annotation2214847/range2214847/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1697/annotation2214847/range2214847/1
type: Range
persistent identity: range2214847
display id: range2214847
version: 1
top level: BBa_M1697
direction: direction/forward
start: 1
end: 79
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation2214848
display id: annotation2214848
version: 1
top level: BBa_M1697
location: https://synbiohub.org/public/igem/BBa_M1697/annotation2214848/range2214848/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_M1697/annotation2214848/range2214848/1
type: Range
persistent identity: range2214848
display id: range2214848
version: 1
top level: BBa_M1697
direction: direction/forward
start: 40
end: 56
orientation: inline

https://synbiohub.org/public/igem/BBa_M1705_sequence/1
type: Sequence
persistent identity: BBa M1705 sequence
display id: BBa_M1705_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catacaacatttttaaaaagcatggtataattactagagttcagggggggaaatactagatggtcaaattaaccgatgtagctgcaaaagcaggctgctctgttactacagtttcacgcgtaattaataattacggctatattagtcaaaaaacacgtaaaaaagttcatgaagcaatgaaagagttaaattatcaacctaattcagtcgctcgatctctacaaggcaagaaaaccaaattaattggtttaatttttcctgatgtttctaatcctttttttggagaattagtttctaaaatagaagatcaactttttaaacacggctataaaacaattctttgtaacgcgggcaatgacaaagaaaaagagcgtacttacctgcaaatgttaatggctaaccaagtcgatggcattattgccggtgcacataacttaggaattgaagagtatcaaagaactggcttaccaattgtatcttttgatcgaaaactatcggacaatattccaatcgttagtgccgataactttaaaggcggtagtttagctacgcaagagctttataatcatggtgcacgtcacatttactttgtaggaaatcccactttagatggtcatcctactcaaaagcggctcctaggctataaagaaacaattaaggaactcaagttaacagaacatattcatccagtgatctttaatgaaaccctcgccttaaaagaaatggcaattagagacttactagaaaatcataaagcagatggaatagtcgcttctgatgacctcactgcccttcttattttaaatatcagtcatgatttaggtattcatattcctaaagatttaaaaattattggctttgatggtagtaaagttgtccgtgattttgaaccacgtttatctacaattgttcaacctattccctcaattgcacaattacttgttaaacttttagaaaaaagaatcgataatccaactgaagaactggataactatacctactatttaccggtagaactacttaaacgtaaatctagtggaaactaatactagagtctcatcgaatacgatgagaaaaataaaactactagagttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaattactagagttcagggggggaaatactagatgttggagaacaagaaccacaagaaaataagcctgagcgggaaatcactgctgatgggaacattatccacggcggcgattgtcctttccgcgagcaccgctaacgccgctaccataaacgcggacaatgtaaatgagaatcaaacggttgaagtaacagcatcttccgtaaataatgaaaacaacaagcaagtgaccgaaaaagatagcgctgacaagtctacctctgatgtagcggaggacgcgaataccaaaaaatccaatgagaacacggaaacgacagaaaagaatacccaaacagtcgttacaaacgcgccggtgtctgatgtgaagaatacgaatactgttaccgctgagactccggttgataaggttgtcaataattccgatcagaaaaccaccaacgctgcaaccacggatactaagaaagacgatgtaaaacaggtagaaaaaaaagattcggttgacaaaactaatgcagaagaaaataaagatagtagcgtaaaacccgctgaaaatgctacgaaagcagagttaaaaggtcaggtcaaagatatagttgaggaatcgggggtggatacgtctaaactaacaaacgatcagatcaacgaactgaacaaaatcaatttctctaaagaagccaaaagcggcacacagttaacatataatgattttaagaagatcgctaaaaccttgatcgagcaggatgcaaggtatgctattccattttttaatgccagcaagattaaaaacatgcccgctgccaaaaccctggatgcgcaatcaggtaaagttgaagac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1703_sequence/1
type: Sequence
persistent identity: BBa M1703 sequence
display id: BBa_M1703_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaattactagagttcagggggggaaatactagatgttggagaacaagaaccacaagaaaataagcctgagcgggaaatcactgctgatgggaacattatccacggcggcgattgtcctttccgcgagcaccgctaacgccgctaccataaacgcggacaatgtaaatgagaatcaaacggttgaagtaacagcatcttccgtaaataatgaaaacaacaagcaagtgaccgaaaaagatagcgctgacaagtctacctctgatgtagcggaggacgcgaataccaaaaaatccaatgagaacacggaaacgacagaaaagaatacccaaacagtcgttacaaacgcgccggtgtctgatgtgaagaatacgaatactgttaccgctgagactccggttgataaggttgtcaataattccgatcagaaaaccaccaacgctgcaaccacggatactaagaaagacgatgtaaaacaggtagaaaaaaaagattcggttgacaaaactaatgcagaagaaaataaagatagtagcgtaaaacccgctgaaaatgctacgaaagcagagttaaaaggtcaggtcaaagatatagttgaggaatcgggggtggatacgtctaaactaacaaacgatcagatcaacgaactgaacaaaatcaatttctctaaagaagccaaaagcggcacacagttaacatataatgattttaagaagatcgctaaaaccttgatcgagcaggatgcaaggtatgctattccattttttaatgccagcaagattaaaaacatgcccgctgccaaaaccctggatgcgcaatcaggtaaagttgaagacctggagatttgggacagttggccggtgcaagacgccaagacgggttacgtttctaattggaacggctatcaattggtaattggcatgatgggagttccgaatgttaacgataatcatatttatcttttatacaataaatatggcgataacgacttcaatcattggaaaaatgcagggccgatctttggtttaggcaccccagtaattcaacagtggtccggctcggcgactttgaataaagatggttcaattcaactgtactatactaaagtggatacatccgataacaacacgaatcatcagaaactggcgtccgcgaccgtgtatctgaatctggaaaaagatcaggataagatttcaatagcacatgtagacaatgaccacattgtttttgaaggtgatggctatcattaccagacctatgatcagtggaaagagactaacaaaggtgctgacaatattgcgatgagagatgcccacgtaattgacgatgacaatggcaataggtacctcgtattcgaggcctccacgggtacagagaattaccaaggtgacgatcagatctaccagtggttaaattacggtggtacgaataaagataacctgggggattttttccaaatcttaagcaacagcgatattaaagacagagctaaatggagtaatgcggcgatagggatcatcaaattaaatgatgatgtgaaaaacccgtccgttgcaaaggtatactcgccgttaatctcggctccgatggtgtctgacgagatagaacgtcccgatgtggttaaactgggcaataaatactatctctttgcggctactagactcaatcgagggtcgaatgacgacgcttggatggccacgaataaagccgtgggcgataatgtcgcaatgattggttacgtaagcgacaacctgacacatggatatgttcctctcaatgagtctggtgttgtgttgacggcttctgttccagctaactggcgtacggccacatattcgtactatgccgttcccgtggaagggcgtgacgatcaactgctgattacctcatatatcactaatcgcggcgaagttgcaggaaaggggatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1704_sequence/1
type: Sequence
persistent identity: BBa M1704 sequence
display id: BBa_M1704_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catacaacatttttaaaaagcatggtataattactagagttcagggggggaaatactagatggtcaaattaaccgatgtagctgcaaaagcaggctgctctgttactacagtttcacgcgtaattaataattacggctatattagtcaaaaaacacgtaaaaaagttcatgaagcaatgaaagagttaaattatcaacctaattcagtcgctcgatctctacaaggcaagaaaaccaaattaattggtttaatttttcctgatgtttctaatcctttttttggagaattagtttctaaaatagaagatcaactttttaaacacggctataaaacaattctttgtaacgcgggcaatgacaaagaaaaagagcgtacttacctgcaaatgttaatggctaaccaagtcgatggcattattgccggtgcacataacttaggaattgaagagtatcaaagaactggcttaccaattgtatcttttgatcgaaaactatcggacaatattccaatcgttagtgccgataactttaaaggcggtagtttagctacgcaagagctttataatcatggtgcacgtcacatttactttgtaggaaatcccactttagatggtcatcctactcaaaagcggctcctaggctataaagaaacaattaaggaactcaagttaacagaacatattcatccagtgatctttaatgaaaccctcgccttaaaagaaatggcaattagagacttactagaaaatcataaagcagatggaatagtcgcttctgatgacctcactgcccttcttattttaaatatcagtcatgatttaggtattcatattcctaaagatttaaaaattattggctttgatggtagtaaagttgtccgtgattttgaaccacgtttatctacaattgttcaacctattccctcaattgcacaattacttgttaaacttttagaaaaaagaatcgataatccaactgaagaactggataactatacctactatttaccggtagaactacttaaacgtaaatctagtggaaactaatactagagtctcatcgaatacgatgagaaaaataaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1696_sequence/1
type: Sequence
persistent identity: BBa M1696 sequence
display id: BBa_M1696_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcaaattaaccgatgtagctgcaaaagcaggctgctctgttactacagtttcacgcgtaattaataattacggctatattagtcaaaaaacacgtaaaaaagttcatgaagcaatgaaagagttaaattatcaacctaattcagtcgctcgatctctacaaggcaagaaaaccaaattaattggtttaatttttcctgatgtttctaatcctttttttggagaattagtttctaaaatagaagatcaactttttaaacacggctataaaacaattctttgtaacgcgggcaatgacaaagaaaaagagcgtacttacctgcaaatgttaatggctaaccaagtcgatggcattattgccggtgcacataacttaggaattgaagagtatcaaagaactggcttaccaattgtatcttttgatcgaaaactatcggacaatattccaatcgttagtgccgataactttaaaggcggtagtttagctacgcaagagctttataatcatggtgcacgtcacatttactttgtaggaaatcccactttagatggtcatcctactcaaaagcggctcctaggctataaagaaacaattaaggaactcaagttaacagaacatattcatccagtgatctttaatgaaaccctcgccttaaaagaaatggcaattagagacttactagaaaatcataaagcagatggaatagtcgcttctgatgacctcactgcccttcttattttaaatatcagtcatgatttaggtattcatattcctaaagatttaaaaattattggctttgatggtagtaaagttgtccgtgattttgaaccacgtttatctacaattgttcaacctattccctcaattgcacaattacttgttaaacttttagaaaaaagaatcgataatccaactgaagaactggataactatacctactatttaccggtagaactacttaaacgtaaatctagtggaaactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1694_sequence/1
type: Sequence
persistent identity: BBa M1694 sequence
display id: BBa_M1694_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcagggggggaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1695_sequence/1
type: Sequence
persistent identity: BBa M1695 sequence
display id: BBa_M1695_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catacaacatttttaaaaagcatggtataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1701_sequence/1
type: Sequence
persistent identity: BBa M1701 sequence
display id: BBa_M1701_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaattactagagttcagggggggaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1698_sequence/1
type: Sequence
persistent identity: BBa M1698 sequence
display id: BBa_M1698_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttggagaacaagaaccacaagaaaataagcctgagcgggaaatcactgctgatgggaacattatccacggcggcgattgtcctttccgcgagcaccgctaacgccgctaccataaacgcggacaatgtaaatgagaatcaaacggttgaagtaacagcatcttccgtaaataatgaaaacaacaagcaagtgaccgaaaaagatagcgctgacaagtctacctctgatgtagcggaggacgcgaataccaaaaaatccaatgagaacacggaaacgacagaaaagaatacccaaacagtcgttacaaacgcgccggtgtctgatgtgaagaatacgaatactgttaccgctgagactccggttgataaggttgtcaataattccgatcagaaaaccaccaacgctgcaaccacggatactaagaaagacgatgtaaaacaggtagaaaaaaaagattcggttgacaaaactaatgcagaagaaaataaagatagtagcgtaaaacccgctgaaaatgctacgaaagcagagttaaaaggtcaggtcaaagatatagttgaggaatcgggggtggatacgtctaaactaacaaacgatcagatcaacgaactgaacaaaatcaatttctctaaagaagccaaaagcggcacacagttaacatataatgattttaagaagatcgctaaaaccttgatcgagcaggatgcaaggtatgctattccattttttaatgccagcaagattaaaaacatgcccgctgccaaaaccctggatgcgcaatcaggtaaagttgaagacctggagatttgggacagttggccggtgcaagacgccaagacgggttacgtttctaattggaacggctatcaattggtaattggcatgatgggagttccgaatgttaacgataatcatatttatcttttatacaataaatatggcgataacgacttcaatcattggaaaaatgcagggccgatctttggtttaggcaccccagtaattcaacagtggtccggctcggcgactttgaataaagatggttcaattcaactgtactatactaaagtggatacatccgataacaacacgaatcatcagaaactggcgtccgcgaccgtgtatctgaatctggaaaaagatcaggataagatttcaatagcacatgtagacaatgaccacattgtttttgaaggtgatggctatcattaccagacctatgatcagtggaaagagactaacaaaggtgctgacaatattgcgatgagagatgcccacgtaattgacgatgacaatggcaataggtacctcgtattcgaggcctccacgggtacagagaattaccaaggtgacgatcagatctaccagtggttaaattacggtggtacgaataaagataacctgggggattttttccaaatcttaagcaacagcgatattaaagacagagctaaatggagtaatgcggcgatagggatcatcaaattaaatgatgatgtgaaaaacccgtccgttgcaaaggtatactcgccgttaatctcggctccgatggtgtctgacgagatagaacgtcccgatgtggttaaactgggcaataaatactatctctttgcggctactagactcaatcgagggtcgaatgacgacgcttggatggccacgaataaagccgtgggcgataatgtcgcaatgattggttacgtaagcgacaacctgacacatggatatgttcctctcaatgagtctggtgttgtgttgacggcttctgttccagctaactggcgtacggccacatattcgtactatgccgttcccgtggaagggcgtgacgatcaactgctgattacctcatatatcactaatcgcggcgaagttgcaggaaaggggatgcacgccacatgggctccctcttttctgctacaaatcaatccggataatacaacaacggtactggccaagatgacgaatcagggagattggatttgggacgattcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1699_sequence/1
type: Sequence
persistent identity: BBa M1699 sequence
display id: BBa_M1699_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtctctgctgatggtcatcgtccgaccaaagcagaattagtccgcttaggaattggcttcactgtgtctgcggtggcctgtgctataccctgggtggccctttccacggttattctgccacgcgtttttgaacttatcgataattccagcaaagaagcgatgcttgggttggtcaactcgatcggtgctatcgttgccctgctcgcaaacatcatttttggtactctgagtgatatgactcgttcccgctttggaaaacgtaccccctggatcgtgggtggcggaattatcacgggtttagccattggcgctatagcgtttacccgtaacgaagccttgattatctttctctggtgcgtggcccagatgggatataacatgatgctggcaccgtatgttgcagtgatgtcggatcgtgtgcctgataaagttcgaggaactatatctggattctacggggccggtattgccgtcggacagactctgggatccgttgttggggccgctttcctgcgacaggggcaaagtggtctgttttctgcctggatgatgggtctgggcatattctctctaacaggtattttcgtggtggctatgtggccgcgtgagaagagttctcgcttcgaagaacagtcagaacatgtaaccgtaaagagtgtacttctgaactttcgtcctcctcgtcatgctccagatttttattacgcacttgttggtaggacactgatgatgggaggttattggatgatcaccacgtaccagctgtatatagcccaggactatatttatgccggagaccccaatgcaaaagacaaagcggcgactattattgctacgatgggtgttattaccttggtggtttcccttatcgcagcagtgactgccggacccataactgatagactgggccgcagaaagctgccagttgcgttagccagttgcctctttgcggtaggtgccgcaatgcccctgttttttcggagccctatgggtatgtatctctttgcagcagtcgccggtttaggctacggtgtctataatgcaatagatcaagcgttaaatgttgctgttctccctaatcctaaagaagcggggaaggatttaggcattctgaatctggcaaataccctttctacggtgattggcaccgcaatgacttctctagttgtttctattgtgaaatcaagcatgcacgtctctactacccctgcggtcgcgtacgcttgggtctttggcgtggccatcgtgattgtcctggccgctgctgcgctgattatgcgcattcgaaacgtgaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1700_sequence/1
type: Sequence
persistent identity: BBa M1700 sequence
display id: BBa_M1700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctcatcgaatacgatgagaaaaataaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1697_sequence/1
type: Sequence
persistent identity: BBa M1697 sequence
display id: BBa_M1697_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaaaattaaaagaaaaatggtatgataaaaagtatattatggaaccggtaccatttaaatgagcttattttataaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z