This is a composite part built from part M1704 (promoter+RBS+scrR+terminator) and part M1703 (promoter+RBS+inulosucrase+RBS+scrT+terminator). With this combination it is now a complete operon for the increased uptake of sucrose into the cell and conversion of sucrose to inulin.
Inulosucrase and scrT sucrose transport protein composite part
inulosucrase enzyme and sucrose transporter scrT downstream of sucrose regulated promoter
ScrR Promoter + RBS + ScrR +Transcription terminator
this is the composite part with promoter, RBS, ScrR and terminator for Inulosucrase to digest fructose into inulin, which will not be absorbed by human body.
BBa_M1695 is ScrR promoter BBa_M1694 RBS BBa_M1696 ScrR BBa_M1700 terminator.
scrR LacI family sucrose operon repressor
scrR sequence found in Lactobacillus sakei subsp. sakei as identified in NCBI gene database
ScrR is in the LacI family for operon repression. It negatively regulates by binding as a repressor to the promoter. In the presence of sucrose it is deactivated and transcription can occur.
Checked for RE sites. Also had to find how it is promoted because it appears to be immediatly downstream from the gene it regulates
Ribosome binding site for ScrR in Lactobacillus sakei subsp. sakei as identified in NCBI gene database.
This ribosome binding site initiates translation for the bacteria Lactobacillus.
It needed to initiate for Lactobacillus.
Promoter for ScrR in Lactobacillus agilis as identified in NCBI gene database.
This promoter is for the bacteria Lactobacillus agilis, it promotes for the protein ScrR at high efficiency.
This is a combination of part M1696 and M1694
This is the promoter to which binds ScrR to regulate the transcription of a gene. We also added onto the end a RBS from Lactobacillus Sakei as identified on the NCBI website.
We needed a promoter that ScrR regulates and a RBS that works in Lactobacillus, so we had to seek those out.
This enzyme can degrade the fructose into inulin, which can not be taken into human body.
Optimized this enzyme in DNA 2.0 for compatibility with E. coli.
This gene sequence codes for the ScrT protein which serves as a symporter for sucrose into the cell. The sequence is originally from [Bifidobacterium animalis subsp. lactis]. The sequence is normally a part of a gene cluster containing scrT, scrP, and scrR. Only scrT was used specifically from these gene cluster in our project to increase the uptake of sucrose by the cell. The sequence present in this part has been optimized for use in Lactobacillus johnsonii.
This sequence was put into DNA 2.0's Gene Designer software to optimize its G-C content for use in Lactobacillus Johnsonii. Also it was checked for comparability with standard 10 restriction enzymes. The sequence here does not contain any restriction site conflicts with standard 10.
From L. johnsonii derived by use of the Webgester DB. http://pallab.serc.iisc.ernet.in/gester/65481116/index.html
A transcription terminator native to L. johnsonii NCC 533. This terminator originally exists downstream of the rpsR coding sequence. It was used in our project downstream from scrT to terminate transcription. This terminator has a deltaG of -12.33.
This sequence was not altered in any way since it was to be used in L. johnsonii and that is the native organism from which it was derived.
contains operator site for sucrose mediated repressor
Conversion of the iGEM parts registry to SBOL2.1