BBa_K844004
1
BBa_K844004
Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Artificially designed based on BBa_K844002 and DNA was synthesized
Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
false
false
_1104_
0
13876
9
In stock
false
none
false
Andrea Halling
annotation2212323
1
Beta-spiral
range2212323
1
46
60
annotation2212332
1
Strength Domain
range2212332
1
186
204
annotation2212327
1
Beta-spiral
range2212327
1
95
110
annotation2212328
1
Beta-spiral
range2212328
1
114
129
annotation2212325
1
Elasticity Domain
range2212325
1
85
168
annotation2212322
1
Beta-spiral
range2212322
1
31
45
annotation2212329
1
Beta-spiral
range2212329
1
130
144
annotation2212326
1
Beta-helix
range2212326
1
85
94
annotation2212331
1
Linker Domain
range2212331
1
169
185
annotation2212320
1
Beta-helix
range2212320
1
1
9
annotation2212319
1
Elasticity Domain
range2212319
1
1
84
annotation2212321
1
Beta-spiral
range2212321
1
10
24
annotation2212324
1
Beta-spiral
range2212324
1
69
84
annotation2212330
1
Beta-spiral
range2212330
1
153
168
BBa_M1718
1
BBa_M1718
Optimized Hepcidin + 1x spider silk
2013-03-31T11:00:00Z
2015-05-08T01:13:58Z
Both protein coding regions are based on original AA sequences, but have been optimized for expression in E. coli.
This part is for a class project and does not really exist. The part combines the part BBa_M1716 that codes for the E. coli optimized hepcidin protein with part BBa_K844004, which codes for a single coding region of spider silk protein.
false
false
_768_
0
16127
9
Not in stock
false
No considerations of note.
false
Richard Decker
component2219119
1
BBa_M1716
component2219134
1
BBa_K844004
annotation2219119
1
BBa_M1716
range2219119
1
1
297
annotation2219134
1
BBa_K844004
range2219134
1
306
509
BBa_M1716
1
BBa_M1716
Hepcidin Optimized for E. coli
2013-03-31T11:00:00Z
2015-05-08T01:13:58Z
NCBI OPTIMIZER using AA sequence from GenBank number ADA68357.
ATGAAAGTTCTGGCGGCGTGCGTTTTCCTGCTGCTGCTGGTTCTGCTGCACGGTTCTCCG
GCGGCGTGCTCTGCGCTGCGTGCGCAGGCGAACATCGGTCTGATGCCGCGTCCGGAAACC
GGTGCGCAGTCTCACGGTCTGGAAGCGGCGGCGGGTCTGATGCCGCACCCGGAAATCGGT
GCGCAGTCTCTGGAAGTTCCGCTGCGTCGTTCTAAACGTTTCAACTCTCACTTCCCGATC
TGCTCTTACTGCTGCAACTGCTGCCGTAACAAAGGTTGCGGTCTGTGCTGCCGTACCTGA
false
false
_768_
0
16127
9
Not in stock
false
Optimized for E. coli K12
false
Richard Decker
BBa_K844004_sequence
1
ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
BBa_M1716_sequence
1
atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacc
BBa_M1718_sequence
1
atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z