Optimized Hepcidin + 6x spider silk + 10x His Tag
Optimized sequence based on AA sequence available on NCBI for hepcidin from crocodile blood.
This part was designed for a class and does not really exist. It is comprised of the part BBa_M1716, which is optimized hepcidin, 6 repeats of the part BBa_K844004, and part BBa_K844000, which is a 10x His tag.
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
Constructed through oligonucleotide annealing
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
The part comes from the AA sequence for hepcidin, but has been optimized for E. coli.
This part was designed for a class and does not really exist. It is comprised of the part BBa_M1718, which is a combination of Optimized hepcidin and spider silk, and 5 repeats of the part BBa_K844004.
Optimized for E. coli using NCBI OPTIMIZER.
Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
Artificially designed based on BBa_K844002 and DNA was synthesized
Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
Both protein coding regions are based on original AA sequences, but have been optimized for expression in E. coli.
This part is for a class project and does not really exist. The part combines the part BBa_M1716 that codes for the E. coli optimized hepcidin protein with part BBa_K844004, which codes for a single coding region of spider silk protein.
NCBI OPTIMIZER using AA sequence from GenBank number ADA68357.
ATGAAAGTTCTGGCGGCGTGCGTTTTCCTGCTGCTGCTGGTTCTGCTGCACGGTTCTCCG GCGGCGTGCTCTGCGCTGCGTGCGCAGGCGAACATCGGTCTGATGCCGCGTCCGGAAACC GGTGCGCAGTCTCACGGTCTGGAAGCGGCGGCGGGTCTGATGCCGCACCCGGAAATCGGT GCGCAGTCTCTGGAAGTTCCGCTGCGTCGTTCTAAACGTTTCAACTCTCACTTCCCGATC TGCTCTTACTGCTGCAACTGCTGCCGTAACAAAGGTTGCGGTCTGTGCTGCCGTACCTGA
Conversion of the iGEM parts registry to SBOL2.1